Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MKS87_RS13975 Genome accession   NZ_CP092631
Coordinates   2673092..2673502 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain YB-15     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2668092..2678502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKS87_RS13945 (MKS87_13945) - 2668179..2668319 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  MKS87_RS13950 (MKS87_13950) yqeF 2668625..2669356 (-) 732 WP_029317882.1 SGNH/GDSL hydrolase family protein -
  MKS87_RS13955 (MKS87_13955) cwlH 2669608..2670360 (-) 753 WP_160221685.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  MKS87_RS13960 (MKS87_13960) yqeD 2670547..2671173 (+) 627 WP_173614194.1 TVP38/TMEM64 family protein -
  MKS87_RS13965 (MKS87_13965) gnd 2671192..2672085 (-) 894 WP_043857850.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  MKS87_RS13970 (MKS87_13970) yqeB 2672337..2673059 (+) 723 WP_010886572.1 hypothetical protein -
  MKS87_RS13975 (MKS87_13975) nucA/comI 2673092..2673502 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  MKS87_RS13980 (MKS87_13980) - 2673698..2674045 (+) 348 Protein_2713 sigma-70 family RNA polymerase sigma factor -
  MKS87_RS13985 (MKS87_13985) spoIVCA 2674076..2675536 (-) 1461 WP_240304280.1 site-specific DNA recombinase SpoIVCA -
  MKS87_RS13990 (MKS87_13990) - 2675494..2675672 (-) 179 Protein_2715 hypothetical protein -
  MKS87_RS13995 (MKS87_13995) - 2676125..2677138 (-) 1014 WP_173614196.1 NADP-dependent oxidoreductase -
  MKS87_RS14000 (MKS87_14000) - 2677568..2677720 (-) 153 WP_260438897.1 hypothetical protein -
  MKS87_RS14005 (MKS87_14005) - 2677736..2678485 (-) 750 WP_173614197.1 SDR family NAD(P)-dependent oxidoreductase -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=659370 MKS87_RS13975 WP_009967785.1 2673092..2673502(-) (nucA/comI) [Bacillus subtilis strain YB-15]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=659370 MKS87_RS13975 WP_009967785.1 2673092..2673502(-) (nucA/comI) [Bacillus subtilis strain YB-15]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCAGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529