Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MKS87_RS00580 Genome accession   NZ_CP092631
Coordinates   105688..107064 (+) Length   458 a.a.
NCBI ID   WP_003242044.1    Uniprot ID   A0A9W5LEJ7
Organism   Bacillus subtilis strain YB-15     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 100688..112064
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKS87_RS00560 (MKS87_00560) ctsR 101041..101505 (+) 465 WP_003225724.1 transcriptional regulator CtsR -
  MKS87_RS00565 (MKS87_00565) mcsA 101519..102076 (+) 558 WP_015252993.1 protein-arginine kinase activator protein McsA -
  MKS87_RS00570 (MKS87_00570) mcsB 102076..103167 (+) 1092 WP_003235007.1 protein arginine kinase -
  MKS87_RS00575 (MKS87_00575) clpC 103164..105596 (+) 2433 WP_014475592.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  MKS87_RS00580 (MKS87_00580) radA 105688..107064 (+) 1377 WP_003242044.1 DNA repair protein RadA Machinery gene
  MKS87_RS00585 (MKS87_00585) disA 107068..108150 (+) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  MKS87_RS00590 (MKS87_00590) yacL 108266..109366 (+) 1101 WP_003235014.1 PIN/TRAM domain-containing protein -
  MKS87_RS00595 (MKS87_00595) ispD 109381..110079 (+) 699 WP_003235019.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MKS87_RS00600 (MKS87_00600) ispF 110072..110548 (+) 477 WP_003225745.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49496.97 Da        Isoelectric Point: 7.9805

>NTDB_id=659320 MKS87_RS00580 WP_003242044.1 105688..107064(+) (radA) [Bacillus subtilis strain YB-15]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSDSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=659320 MKS87_RS00580 WP_003242044.1 105688..107064(+) (radA) [Bacillus subtilis strain YB-15]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
TGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTCAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATTGGCGGTGATCCTGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCAGACTCATCAAACAGTGTCCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTGCGAGCAGACCGTCTCGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATTACATCGGC
TCCAGGCAGTGTGTCACAGGTCAGAGAATGTACCGCTGAGCTGATGAAAATTGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTGACGAAAGAGGGGTCTATTGCAGGTCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGAGAACGCCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGATCCACAAACGAAATGGGCATTTT
CGAAATGCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCGGAAATTTTCTTAGAAGAACGCTCTGCTGGTTCCGCAG
GCTCGAGTATCACTGCCTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCGCTCATCTCGCCAACAAGCTTT
GGCAACCCAAGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCTTTGTTAATGGCTGTGTTAGAAAAAAGAGTTGG
ACTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCCA
TTGCGATTAGTATCGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGTTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGTTTTAAACGCATGATCATACC
CGCGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.345

100

0.993
  radA Streptococcus pneumoniae Rx1

63.355

98.908

0.627
  radA Streptococcus pneumoniae D39

63.355

98.908

0.627
  radA Streptococcus pneumoniae R6

63.355

98.908

0.627
  radA Streptococcus pneumoniae TIGR4

63.355

98.908

0.627
  radA Streptococcus mitis NCTC 12261

63.355

98.908

0.627
  radA Streptococcus mitis SK321

63.135

98.908

0.624