Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MKF35_RS11970 Genome accession   NZ_CP092629
Coordinates   2587040..2588401 (+) Length   453 a.a.
NCBI ID   WP_017002524.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain HU20     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2582040..2593401
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKF35_RS11935 - 2582137..2582913 (+) 777 WP_003456796.1 ferredoxin--NADP reductase -
  MKF35_RS11940 mscL 2583018..2583431 (-) 414 WP_003104883.1 large-conductance mechanosensitive channel protein MscL -
  MKF35_RS11945 - 2583474..2583962 (+) 489 WP_169433242.1 hypothetical protein -
  MKF35_RS11950 katB 2583959..2585500 (+) 1542 WP_003094881.1 catalase KatB -
  MKF35_RS11955 - 2585554..2586105 (+) 552 WP_031772008.1 ankyrin repeat domain-containing protein -
  MKF35_RS11960 - 2586180..2586434 (+) 255 WP_003104887.1 YdcH family protein -
  MKF35_RS11965 - 2586496..2586930 (-) 435 WP_003112796.1 CopD family protein -
  MKF35_RS11970 radA 2587040..2588401 (+) 1362 WP_017002524.1 DNA repair protein RadA Machinery gene
  MKF35_RS11975 mapZ 2588408..2588785 (-) 378 WP_003094864.1 cyclic di-GMP-binding protein MapZ -
  MKF35_RS11980 - 2588988..2589494 (+) 507 WP_003094862.1 DUF3015 domain-containing protein -
  MKF35_RS11985 - 2589832..2591898 (+) 2067 WP_003094859.1 carbon starvation CstA family protein -
  MKF35_RS11990 - 2591947..2592150 (+) 204 WP_003094856.1 YbdD/YjiX family protein -
  MKF35_RS11995 yjiA 2592210..2593214 (+) 1005 WP_003094852.1 GTPase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48380.78 Da        Isoelectric Point: 6.8357

>NTDB_id=659278 MKF35_RS11970 WP_017002524.1 2587040..2588401(+) (radA) [Pseudomonas aeruginosa strain HU20]
MAKAKRMYGCTECGATFPKWAGQCADCGAWNTLVETVVEAAPSGSGRGGWAGQQANLKTLAEVSVEEMPRFTTGSAELDR
VLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNLASRVPALYVTGEESQQQVAMRARRLSLPEDKLKVMTETSIETIIATA
RQEQPRVMVIDSIQTIFTEQLQSAPGGVAQVRESAAMLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFEGE
SDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEAVPGSVVMATWEGSRPMLVEVQALVDTSHLANP
RRVTLGLDQNRLAMLLAVLHRHGGIPTYDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSGEV
RPVPSGQERLKEAGKHGFKRAIVPLGNAPKEAPAGLQVIAVTRLEQALDALFE

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=659278 MKF35_RS11970 WP_017002524.1 2587040..2588401(+) (radA) [Pseudomonas aeruginosa strain HU20]
ATGGCCAAGGCCAAGCGCATGTACGGCTGCACCGAGTGTGGCGCGACCTTCCCGAAATGGGCCGGCCAGTGCGCGGACTG
CGGGGCCTGGAACACCCTGGTGGAGACCGTCGTCGAGGCCGCGCCGAGCGGCTCCGGGCGCGGCGGCTGGGCCGGCCAGC
AGGCCAACCTGAAGACCCTGGCGGAGGTCAGCGTCGAGGAAATGCCGCGCTTCACGACCGGCTCCGCCGAACTGGACCGG
GTTCTCGGCGGCGGCCTGGTGGACGGTTCGGTGGTGCTGATCGGCGGCGATCCCGGCATCGGCAAGTCGACCATTCTCCT
GCAGACCCTTTGCAACCTGGCCAGCCGCGTCCCGGCGCTGTACGTCACCGGAGAGGAATCCCAGCAGCAGGTGGCGATGC
GCGCGCGGCGCCTGTCGTTGCCGGAAGACAAGCTCAAGGTGATGACCGAGACCAGCATCGAGACCATCATCGCCACCGCG
CGCCAGGAGCAGCCGCGGGTGATGGTGATCGACTCGATCCAGACCATCTTCACCGAGCAGTTGCAATCGGCGCCGGGCGG
CGTCGCCCAGGTCCGCGAGAGCGCCGCGATGCTGGTGCGCTATGCCAAGCAGAGCGGCACGGCGATCTTTCTGGTCGGCC
ACGTGACCAAGGAAGGCGCCCTGGCCGGCCCGCGGGTGCTCGAACACATGGTCGACACCGTACTGTATTTCGAGGGCGAA
TCAGACGGCCGCCTGCGCCTGCTGCGGGCGGTGAAGAACCGCTTCGGCGCGGTCAACGAACTGGGAGTGTTCGGCATGAC
CGACAAAGGCCTGAAGGAAGTGAGCAACCCTTCGGCGATCTTCCTCACGCGGGCCCAGGAAGCGGTGCCCGGCAGCGTGG
TGATGGCCACCTGGGAAGGCTCGCGACCGATGCTGGTGGAGGTCCAGGCGCTGGTCGACACCAGTCACCTGGCGAATCCG
CGGCGGGTGACCCTGGGCCTCGACCAGAACCGCCTGGCGATGCTGCTGGCGGTGCTGCACCGGCACGGTGGTATCCCGAC
CTACGACCAGGACGTGTTCCTCAACGTGGTGGGTGGGGTCAAGGTGCTGGAAACCGCCTCCGACCTGGCGTTGATGGCGG
CGGTGATGTCCAGCCTGCGCAACCGCCCGCTGCCGCACGACCTGCTGGTGTTCGGCGAGGTCGGGCTGTCCGGCGAGGTG
CGTCCGGTGCCGAGCGGCCAGGAGCGCCTGAAGGAAGCCGGCAAGCATGGTTTCAAGCGTGCCATCGTACCCCTCGGCAA
CGCGCCGAAGGAGGCGCCGGCGGGATTGCAGGTGATCGCCGTGACGCGCCTCGAGCAGGCCCTGGACGCCCTCTTCGAGT
GA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(75-219)

Subunit ChlI of Mg-chelatase (ChlI)

(361-427)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.458

100

0.486
  radA Streptococcus pneumoniae Rx1

46.288

100

0.468
  radA Streptococcus pneumoniae D39

46.288

100

0.468
  radA Streptococcus pneumoniae R6

46.288

100

0.468
  radA Streptococcus pneumoniae TIGR4

46.288

100

0.468
  radA Streptococcus mitis SK321

46.389

100

0.468
  radA Streptococcus mitis NCTC 12261

46.171

100

0.466