Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AMJAP_RS04805 Genome accession   NZ_AP014545
Coordinates   1047284..1048510 (-) Length   408 a.a.
NCBI ID   WP_019621937.1    Uniprot ID   A0A7R6P8S7
Organism   Amphritea japonica ATCC BAA-1530     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1042284..1053510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMJAP_RS04780 (AMJAP_0920) argJ 1043218..1044435 (+) 1218 WP_019621932.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  AMJAP_RS04785 (AMJAP_0921) - 1044451..1045398 (+) 948 WP_019621933.1 Nudix family hydrolase -
  AMJAP_RS04790 (AMJAP_0922) yacG 1045430..1045669 (-) 240 WP_019621934.1 DNA gyrase inhibitor YacG -
  AMJAP_RS04795 (AMJAP_0923) coaE 1045671..1046279 (-) 609 WP_019621935.1 dephospho-CoA kinase -
  AMJAP_RS04800 (AMJAP_0924) pilD 1046316..1047212 (-) 897 WP_019621936.1 prepilin peptidase Machinery gene
  AMJAP_RS04805 (AMJAP_0925) pilC 1047284..1048510 (-) 1227 WP_019621937.1 type II secretion system F family protein Machinery gene
  AMJAP_RS04810 (AMJAP_0926) pilB 1048588..1050306 (-) 1719 WP_019621938.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AMJAP_RS17820 (AMJAP_0927) - 1050559..1051059 (+) 501 WP_019621939.1 pilin -
  AMJAP_RS04820 (AMJAP_0928) - 1051149..1051508 (+) 360 WP_019621940.1 hypothetical protein -
  AMJAP_RS04825 (AMJAP_0929) nadC 1051699..1052544 (-) 846 WP_019621941.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AMJAP_RS04830 (AMJAP_0930) ampD 1052710..1053288 (+) 579 WP_019621942.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44140.57 Da        Isoelectric Point: 9.4594

>NTDB_id=65857 AMJAP_RS04805 WP_019621937.1 1047284..1048510(-) (pilC) [Amphritea japonica ATCC BAA-1530]
MAKKVTDKAPITFNWEGKDKSGNPSKGSINADNLGEAKALLRKQGVMPKKVRKQTYSMFSNHNKPIKPLDVALFTRQTAT
MLKSGVPLLQGFDIVANGIEKAKMKKVIYDLKSDVSGGTSFAKALERHPKCFDDLYCNLVDAGESSGSLETMLDRIASYK
EKMEKLKKKIKKALTYPIAVIVVGLVVSAILLIKVVPQFESVFSSFGADLPAFTLFVVSLSNIAQDWWFIAIIAIAVSGF
TISKAHEKSRGFREWVDRSVLKLPIIGQILHNSAIARFARTLATTFAAGVPLVDALDSAAGASGNIVYETAIQQIKNGVS
TGQSLQNAVTMTGLFPPMATQMIAIGEEAGSLETMLDKVAGYYEDEVDDAVDNLSTLIEPLIMSILGILVGGLVIAMYLP
IFQLGNVV

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=65857 AMJAP_RS04805 WP_019621937.1 1047284..1048510(-) (pilC) [Amphritea japonica ATCC BAA-1530]
ATGGCTAAGAAAGTAACAGATAAGGCACCTATCACCTTTAACTGGGAAGGCAAGGATAAAAGTGGTAATCCTAGTAAAGG
CTCTATCAATGCCGATAATCTTGGCGAAGCTAAAGCCCTGTTGCGCAAACAGGGAGTTATGCCGAAAAAAGTCCGCAAAC
AGACTTATAGCATGTTCAGCAATCACAACAAACCGATAAAACCACTGGACGTTGCGCTGTTCACACGACAAACCGCCACC
ATGCTGAAATCTGGCGTCCCCCTATTACAAGGCTTCGATATTGTTGCTAACGGCATAGAAAAAGCCAAAATGAAAAAAGT
TATTTATGACCTAAAATCCGACGTCAGCGGCGGTACCAGTTTTGCCAAAGCACTAGAACGGCACCCAAAGTGCTTTGATG
ATCTCTATTGTAATCTGGTAGATGCTGGTGAAAGCTCCGGTTCGCTTGAAACCATGTTAGACCGTATTGCATCTTACAAA
GAGAAGATGGAAAAACTTAAGAAAAAAATAAAGAAAGCACTTACCTATCCAATTGCCGTTATCGTAGTCGGTTTAGTTGT
TTCAGCAATACTGCTAATTAAAGTAGTACCACAATTCGAAAGTGTATTTTCCAGCTTCGGCGCAGACCTGCCAGCATTCA
CTTTATTTGTAGTCAGCCTGTCTAATATAGCCCAGGACTGGTGGTTTATCGCAATTATCGCAATCGCAGTATCTGGTTTT
ACAATATCTAAAGCGCATGAGAAATCACGAGGCTTCAGAGAGTGGGTAGATCGAAGCGTTCTCAAACTGCCCATTATTGG
CCAGATACTTCACAACTCCGCCATTGCCCGTTTCGCCAGAACTCTGGCAACTACTTTTGCAGCTGGCGTTCCTTTAGTTG
ATGCTCTGGACTCTGCTGCAGGCGCTTCAGGAAATATTGTCTATGAAACAGCCATTCAGCAGATTAAGAATGGCGTATCG
ACTGGTCAGTCACTTCAAAACGCAGTTACTATGACCGGCCTGTTCCCACCGATGGCAACACAAATGATCGCTATCGGCGA
AGAAGCGGGCTCGCTTGAGACCATGCTAGATAAAGTTGCTGGTTATTATGAAGATGAGGTTGATGATGCCGTCGACAACC
TATCAACGTTAATCGAACCGTTAATCATGTCAATCCTGGGCATTTTGGTAGGCGGCCTGGTTATCGCGATGTACTTACCA
ATCTTCCAACTAGGTAATGTAGTTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(278-400)

Type II secretion system (T2SS), protein F (T2SSF)

(74-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7R6P8S7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.598

100

0.576
  pilC Acinetobacter baylyi ADP1

56.691

100

0.571
  pilC Acinetobacter baumannii D1279779

55.012

100

0.551
  pilC Legionella pneumophila strain ERS1305867

55.276

97.549

0.539
  pilC Vibrio cholerae strain A1552

41.667

100

0.417
  pilG Neisseria gonorrhoeae MS11

42.569

97.304

0.414
  pilG Neisseria meningitidis 44/76-A

42.569

97.304

0.414
  pilC Vibrio campbellii strain DS40M4

41.855

97.794

0.409
  pilC Thermus thermophilus HB27

36.935

97.549

0.36

Multiple sequence alignment