Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   H7R08_RS02015 Genome accession   NZ_AP022095
Coordinates   452081..452758 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain WP4-S18-ESBL-08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 447081..457758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R08_RS02000 (WP4S18E08_03950) chiA 448448..451141 (+) 2694 WP_000773143.1 bifunctional chitinase/lysozyme -
  H7R08_RS02005 (WP4S18E08_03960) bfd 451310..451504 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  H7R08_RS02010 (WP4S18E08_03970) bfr 451576..452052 (+) 477 WP_000675504.1 bacterioferritin -
  H7R08_RS02015 (WP4S18E08_03980) pilD 452081..452758 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  H7R08_RS02020 (WP4S18E08_03990) gspM 452758..453219 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  H7R08_RS02025 (WP4S18E08_04000) gspL 453216..454379 (-) 1164 WP_001115228.1 type II secretion system protein GspL -
  H7R08_RS02030 (WP4S18E08_04010) gspK 454394..455377 (-) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  H7R08_RS02035 (WP4S18E08_04020) gspJ 455370..455957 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  H7R08_RS02040 (WP4S18E08_04030) gspI 455950..456327 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  H7R08_RS02045 (WP4S18E08_04040) gspH 456324..456833 (-) 510 WP_001076029.1 type II secretion system minor pseudopilin GspH -
  H7R08_RS02050 (WP4S18E08_04050) gspG 456841..457278 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=65854 H7R08_RS02015 WP_000178161.1 452081..452758(-) (pilD) [Escherichia coli strain WP4-S18-ESBL-08]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=65854 H7R08_RS02015 WP_000178161.1 452081..452758(-) (pilD) [Escherichia coli strain WP4-S18-ESBL-08]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment