Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   MJ920_RS05565 Genome accession   NZ_CP092499
Coordinates   1069268..1072972 (+) Length   1234 a.a.
NCBI ID   WP_032857796.1    Uniprot ID   -
Organism   Bacillus velezensis strain YC_89     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1064268..1077972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ920_RS05550 (MJ920_05560) - 1064348..1064857 (-) 510 WP_003155151.1 ferritin-like domain-containing protein -
  MJ920_RS05555 (MJ920_05565) - 1064926..1065639 (-) 714 WP_021734194.1 DUF421 domain-containing protein -
  MJ920_RS05560 (MJ920_05570) addB 1065781..1069281 (+) 3501 WP_003155148.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MJ920_RS05565 (MJ920_05575) addA 1069268..1072972 (+) 3705 WP_032857796.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MJ920_RS05570 (MJ920_05580) sbcD 1073037..1074209 (+) 1173 WP_003155146.1 exonuclease subunit SbcD -
  MJ920_RS05575 (MJ920_05585) sbcC 1074206..1077598 (+) 3393 WP_143255783.1 exonuclease subunit SbcC -
  MJ920_RS05580 (MJ920_05590) - 1077614..1077907 (+) 294 WP_003155144.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141217.61 Da        Isoelectric Point: 5.1579

>NTDB_id=658164 MJ920_RS05565 WP_032857796.1 1069268..1072972(+) (addA) [Bacillus velezensis strain YC_89]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITAEEDPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELAKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FFELADRYTTDRHDLDLQDLVKRVYEYSRSHPDPEAWLQSFVRLYDVTEESKMEELPFYQYVREDAEMALFGAKQKLEKA
LELTKAPGGPAPRAGNFLDDLQQIEELISCRHDFDALYERVPAVSFKRAKPVKGDEFDKALLDEATDLRNGAKKLIEKLK
TDYFTRSPQDHLKSLTDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAVDEEGRRVPSEAAVYYQDQ
FHEVLVDEYQDTNLVQESILQLVKSGDEEAGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTESGEGAGQKIDLNQNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNDAAKTELLLIDSADDADSSEDAEDFETVHWEAKAIAGEIR
KLVSSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDI
PLASVLRSPIVGCDENELALIRLEKKKAPFYEALKAYIANADRHDELYQKLRTFYDSLQKWRSFSTNHSVSELIWEVYRD
TGYFDYAGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKNREKQLAKWQAQADRADWLLSEFDRYQASSYLDFIGPALIRHRDMEAHRTPGLSSTEDIARDPSRFHIRMLQQSE
LLEENPKERAEEKSKRLKAIQQGEPIPGSFSFDDQARRLLEWEYPYRELTAIRTKQSVSELKRKQEYEDEYSGRSLIKPS
GDTLLYRRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHIPTAEEAERTVRMLYEKELLTEEQQEAIDIEEIVRFFGTEIGK
DLLGALRIDREVPFSMALPAGEVYKDAETAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYE
TQIELYTKAVEQITKTKVKGRALYFFDGGHVLTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=658164 MJ920_RS05565 WP_032857796.1 1069268..1072972(+) (addA) [Bacillus velezensis strain YC_89]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
TGTCGCTGCCGCAGCCGGATCAGGTAAAACGGCCGTGCTCGTGGAACGGATGATCAGAAAGATAACCGCGGAGGAAGATC
CTGTTGATGTTGACAGGCTCCTTGTGGTGACCTTTACAAACGCCTCAGCAGCGGAGATGAAGCATCGGATCGCTGAAGCA
TTAGAAAAAGAGCTCGCCAAAAATCCGGGATCTCTGCATATCAGACGGCAGCTCTCTCTATTAAATCGGGCGAGCATTTC
GACGCTTCACTCTTTCTGTCTGCAAGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCCGGTTTTCGGATGGCTG
ACCAGACGGAAGGCGAATTGCTCGGAGACGAAGTGCTTGATGAGCTGTTTGAAGATGAATACGCAAAAGGGAATCAGGCG
TTTTTTGAGCTTGCGGACAGATATACGACAGACAGGCATGATCTGGATCTTCAAGACCTTGTCAAACGGGTGTACGAGTA
TTCCCGGTCGCATCCTGACCCGGAAGCGTGGCTGCAGAGCTTTGTCCGCTTGTATGACGTGACGGAAGAAAGCAAAATGG
AAGAGCTTCCGTTTTACCAGTATGTGAGAGAAGATGCGGAAATGGCGCTCTTCGGGGCAAAACAAAAGCTTGAGAAGGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCCCCGCGCGCTGGTAATTTTCTGGATGACCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTCGATGCGCTGTATGAACGGGTTCCCGCAGTCTCATTTAAGCGGGCGAAGCCGGTAAAGG
GTGATGAATTTGACAAAGCGCTTTTGGACGAAGCGACCGATTTGCGAAACGGCGCGAAGAAACTGATCGAAAAGTTAAAG
ACCGATTACTTTACAAGAAGTCCGCAAGATCACTTGAAGAGTTTAACAGATATGAAGCCTGTCATCGAAACGCTCGTTCA
GCTCGTGATATCGTACGGAAAACGATTTGAAGCGGCGAAACAAGAAAAATCAATTATTGATTTCTCAGATTTGGAACATT
ACTGCCTGGCGATTCTAACTGCGGTTGATGAAGAAGGCCGGCGGGTGCCGAGCGAAGCGGCCGTTTATTATCAAGACCAG
TTTCATGAAGTTCTGGTTGATGAATATCAAGATACCAATCTTGTGCAGGAGTCTATCCTGCAGCTTGTGAAAAGCGGGGA
TGAAGAGGCGGGAAATCTGTTTATGGTAGGCGACGTCAAACAATCGATCTACCGTTTCAGACTTGCTGAGCCGCTGCTGT
TTCTCGGGAAATATAAAAGGTTCACAGAAAGCGGGGAAGGCGCGGGGCAGAAAATTGACCTGAACCAAAATTTCCGCAGC
CGTTCCGATATTTTGGACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGAAAAATCGGCGAAGTGGACTACGATGA
ACAGGCGGCATTGAAGCTCGGCGCATCGTATCCGCCGAATGACGCGGCAAAAACGGAACTGCTGCTGATTGACAGCGCTG
ATGACGCAGACAGCTCCGAGGATGCCGAAGACTTTGAAACCGTGCATTGGGAAGCGAAAGCGATAGCCGGGGAGATCCGA
AAGCTTGTCTCATCTCCGTTTAAGGTCTATGACGGGAAAACAAAAACACACCGGAATATCCAGTACCGGGATATCGTGAT
TTTGCTGCGCTCCATGCCGTGGGCGCCGCAATTAATGGAAGAGCTGAAAAACCAGGGCATTCCGGTGTACGCCAATTTGA
CGTCGGGTTACTTTGAAGCCGTGGAGGTGGCTGCGGCGCTTTCAGTCTTGAAGGTGATTGATAATCCGTATCAGGATATT
CCGCTTGCTTCCGTGCTGCGCTCGCCCATCGTCGGCTGTGACGAAAATGAACTTGCGCTGATACGCCTTGAAAAGAAAAA
AGCGCCGTTTTATGAAGCGCTGAAAGCATATATAGCGAACGCTGACAGACATGATGAACTGTATCAAAAACTGAGAACCT
TTTATGACAGTCTGCAAAAGTGGCGGTCATTTTCGACGAATCATTCGGTGTCTGAGCTGATTTGGGAAGTATACCGCGAT
ACCGGATATTTTGACTATGCAGGCGGCATGCCCGGCGGAAAGCAGCGCCAGGCCAATCTGCGCGTCTTGTATGACCGGGC
GCGCTCGTACGAAGCGACGGCGTTCCGCGGGTTATTCCGCTTTTTGCGGTTTATTGAACGGATGCAGGAGCGCGGCGATG
ATCTGGGGACCGCCCGTGCGCTCAGCGAGCAGGAGGATGTCGTTCGGCTGATGACGATCCACAGCAGCAAAGGACTTGAA
TTTCCCGTCGTTTTCACCGCCGGACTCGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATACCTGCTTGATAAAGA
GCTCGGTTTCGGCACGAAGTATATTCATCCGGAACTCAGAATCAGCTATCCGACTCTTCCGCTTGTGGCGATGAAGAAAA
AGATGCGCAGAGAGCTCCTATCCGAAGAATTGCGCGTATTGTATGTGGCGCTGACCAGAGCGAAGGAAAAGCTGTTTCTC
GTCGGTTCCTGCAAAAACCGGGAAAAACAGCTGGCCAAATGGCAGGCGCAAGCAGACCGGGCCGATTGGCTTCTTTCTGA
ATTCGACCGCTATCAGGCGTCATCTTATTTGGACTTTATCGGGCCGGCGCTCATCCGCCACCGTGACATGGAGGCGCACA
GGACGCCGGGACTTTCTTCGACTGAGGATATCGCGCGCGACCCGTCGCGTTTTCACATCCGGATGCTGCAGCAAAGTGAA
TTGCTGGAAGAGAATCCAAAAGAACGTGCTGAAGAAAAGAGCAAACGTCTCAAGGCGATACAGCAGGGCGAGCCGATTCC
TGGTTCGTTTTCATTTGATGATCAGGCGCGCCGTCTGCTTGAATGGGAATATCCGTACCGAGAGCTGACGGCTATCAGAA
CGAAGCAGTCAGTTTCTGAGCTGAAGAGAAAGCAAGAGTACGAGGATGAATACAGCGGCCGCTCTCTCATCAAACCGTCA
GGAGACACGCTTTTATACAGGCGTCCCGGTTTTATGATGAAAAAAGGGCTGACAGCCGCCGAAAAAGGGACGGCGATGCA
TACGGTGATGCAGCACATACCGCTGACGCACATTCCGACTGCCGAAGAAGCAGAGCGGACGGTCCGGATGCTTTACGAAA
AAGAGCTGCTGACAGAAGAGCAGCAGGAAGCCATTGATATAGAAGAAATTGTCCGGTTTTTCGGAACTGAAATCGGTAAA
GACCTTCTCGGGGCGCTGCGGATTGACCGGGAAGTGCCGTTCAGTATGGCGCTTCCGGCCGGCGAGGTGTACAAGGATGC
CGAAACGGCCGGTGAACCGCTGCTCGTGCAAGGGATTATTGACTGCCTCTACGAAACTGCGGACGGTCTCTATTTGCTGG
ACTATAAAACAGATCGGATAGAGGGGAAATTCCGGAATGGATTTGAAGGCGCGGCTCCGATTCTGCAAAAAAGATATGAA
ACACAAATCGAATTGTATACAAAAGCGGTTGAACAAATTACAAAAACGAAAGTGAAAGGGCGTGCGCTCTATTTCTTCGA
TGGAGGTCACGTTCTTACACTGTAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

82.415

100

0.824