Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   MJE83_RS19475 Genome accession   NZ_CP092446
Coordinates   3847521..3848666 (+) Length   381 a.a.
NCBI ID   WP_015240777.1    Uniprot ID   -
Organism   Bacillus velezensis strain R-71003     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3842521..3853666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJE83_RS19455 (MJE83_19455) - 3843418..3843846 (-) 429 WP_007407634.1 DUF1934 domain-containing protein -
  MJE83_RS19460 (MJE83_19460) - 3844000..3844512 (+) 513 Protein_3796 bacteriocin biosynthesis protein AlbG -
  MJE83_RS19465 (MJE83_19465) - 3844519..3845895 (-) 1377 WP_046702210.1 YncE family protein -
  MJE83_RS19470 (MJE83_19470) - 3845935..3847290 (-) 1356 WP_015240776.1 YncE family protein -
  MJE83_RS19475 (MJE83_19475) rapF 3847521..3848666 (+) 1146 WP_015240777.1 tetratricopeptide repeat protein Regulator
  MJE83_RS19480 (MJE83_19480) phrF 3848650..3848769 (+) 120 WP_015240778.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  MJE83_RS19485 (MJE83_19485) - 3848967..3849170 (+) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  MJE83_RS19490 (MJE83_19490) - 3849184..3849387 (+) 204 WP_007407641.1 hypothetical protein -
  MJE83_RS19495 (MJE83_19495) - 3849422..3849607 (+) 186 WP_007407642.1 DUF4177 domain-containing protein -
  MJE83_RS19500 (MJE83_19500) - 3849793..3850074 (+) 282 WP_007407643.1 hypothetical protein -
  MJE83_RS19505 (MJE83_19505) - 3850118..3850630 (+) 513 WP_031304384.1 stage II sporulation protein M -
  MJE83_RS19510 (MJE83_19510) - 3851221..3851439 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  MJE83_RS19515 (MJE83_19515) speB 3851607..3852479 (-) 873 WP_095352572.1 agmatinase -
  MJE83_RS19520 (MJE83_19520) speE 3852538..3853368 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45572.57 Da        Isoelectric Point: 4.5155

>NTDB_id=657610 MJE83_RS19475 WP_015240777.1 3847521..3848666(+) (rapF) [Bacillus velezensis strain R-71003]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYEIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIVEYEFLESLYVGEPDEEAI
MECFDFLRDKSMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=657610 MJE83_RS19475 WP_015240777.1 3847521..3848666(+) (rapF) [Bacillus velezensis strain R-71003]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTTGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAACAGACATATTTTTCT
ATGGATTATGCCCGGCAGGCTTATGAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCACAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAGTATGAATTTTTGGAATCTTTGTATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATCGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(190-253)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.74

100

0.787
  rapC Bacillus subtilis subsp. subtilis str. 168

57.105

99.738

0.57