Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MJE83_RS18550 Genome accession   NZ_CP092446
Coordinates   3660145..3661530 (-) Length   461 a.a.
NCBI ID   WP_124692700.1    Uniprot ID   -
Organism   Bacillus velezensis strain R-71003     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3655145..3666530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJE83_RS18515 (MJE83_18515) flgL 3655320..3656237 (-) 918 WP_007407477.1 flagellar hook-associated protein FlgL -
  MJE83_RS18520 (MJE83_18520) flgK 3656249..3657766 (-) 1518 WP_025285351.1 flagellar hook-associated protein FlgK -
  MJE83_RS18525 (MJE83_18525) - 3657782..3658264 (-) 483 WP_003151394.1 flagellar protein FlgN -
  MJE83_RS18530 (MJE83_18530) flgM 3658279..3658545 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  MJE83_RS18535 (MJE83_18535) - 3658615..3659034 (-) 420 WP_012118571.1 TIGR03826 family flagellar region protein -
  MJE83_RS18540 (MJE83_18540) comFC 3659108..3659797 (-) 690 WP_199022660.1 phosphoribosyltransferase family protein Machinery gene
  MJE83_RS18545 (MJE83_18545) - 3659803..3660087 (-) 285 WP_007407481.1 competence protein ComFB -
  MJE83_RS18550 (MJE83_18550) comFA 3660145..3661530 (-) 1386 WP_124692700.1 DEAD/DEAH box helicase Machinery gene
  MJE83_RS18555 (MJE83_18555) - 3661639..3662487 (-) 849 WP_003151387.1 DegV family protein -
  MJE83_RS18560 (MJE83_18560) degU 3662585..3663274 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  MJE83_RS18565 (MJE83_18565) degS 3663351..3664514 (-) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  MJE83_RS18570 (MJE83_18570) - 3664737..3665384 (+) 648 WP_007614107.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52722.21 Da        Isoelectric Point: 10.1794

>NTDB_id=657604 MJE83_RS18550 WP_124692700.1 3660145..3661530(-) (comFA) [Bacillus velezensis strain R-71003]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRIKRFIEKEYITAEPSIIRRKNRYVCQRCGQSDQACFAAFWAPSAKRQIT
YCRACVMMGRADELTSLYYWNQAYENSWEPVKLSWEGTLTDGQKRAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQSQLPVFLFVPSVHVLKKTTDYFQKLNVRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGITSGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=657604 MJE83_RS18550 WP_124692700.1 3660145..3661530(-) (comFA) [Bacillus velezensis strain R-71003]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
TTCAGACCAGCGCATCAAGCGTTTTATAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCTGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTATTGGAATCAGGCCTATGAAAACAG
CTGGGAACCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCGGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGTCTGACTCCGTTAATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACCGATTGGGTC
CGCCAAAAACTTCAATCACAGCTGCCGGTTTTCTTATTCGTTCCGTCTGTACATGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAATGTAAGGGCAGAAGGCGTTCATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAGACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACAGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATCACAAGCGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(328-421)

Type III restriction enzyme, res subunit (ResIII)

(118-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.609

99.783

0.625
  comFA Latilactobacillus sakei subsp. sakei 23K

39.952

90.672

0.362