Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   MJE83_RS13600 Genome accession   NZ_CP092446
Coordinates   2713790..2714827 (-) Length   345 a.a.
NCBI ID   WP_218791295.1    Uniprot ID   -
Organism   Bacillus velezensis strain R-71003     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2708790..2719827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJE83_RS13550 (MJE83_13550) - 2709024..2709809 (-) 786 WP_007408329.1 TasA family protein -
  MJE83_RS13555 (MJE83_13555) - 2709874..2710458 (-) 585 WP_015240205.1 signal peptidase I -
  MJE83_RS13560 (MJE83_13560) tapA 2710430..2711101 (-) 672 WP_124692843.1 amyloid fiber anchoring/assembly protein TapA -
  MJE83_RS13565 (MJE83_13565) - 2711360..2711689 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  MJE83_RS13570 (MJE83_13570) - 2711729..2711908 (-) 180 WP_003153093.1 YqzE family protein -
  MJE83_RS13575 (MJE83_13575) comGG 2711965..2712342 (-) 378 WP_015240208.1 competence type IV pilus minor pilin ComGG Machinery gene
  MJE83_RS13580 (MJE83_13580) comGF 2712343..2712843 (-) 501 WP_256994853.1 competence type IV pilus minor pilin ComGF -
  MJE83_RS13585 (MJE83_13585) comGE 2712752..2713066 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  MJE83_RS13590 (MJE83_13590) comGD 2713050..2713487 (-) 438 WP_015240210.1 competence type IV pilus minor pilin ComGD Machinery gene
  MJE83_RS13595 (MJE83_13595) comGC 2713477..2713785 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  MJE83_RS13600 (MJE83_13600) comGB 2713790..2714827 (-) 1038 WP_218791295.1 competence type IV pilus assembly protein ComGB Machinery gene
  MJE83_RS13605 (MJE83_13605) comGA 2714814..2715884 (-) 1071 WP_124692840.1 competence type IV pilus ATPase ComGA Machinery gene
  MJE83_RS13610 (MJE83_13610) - 2716077..2717027 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  MJE83_RS13615 (MJE83_13615) - 2717173..2718474 (+) 1302 WP_012117986.1 hemolysin family protein -
  MJE83_RS13620 (MJE83_13620) - 2718512..2719342 (-) 831 WP_007408317.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39501.78 Da        Isoelectric Point: 9.5935

>NTDB_id=657584 MJE83_RS13600 WP_218791295.1 2713790..2714827(-) (comGB) [Bacillus velezensis strain R-71003]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLKLQLHKRQLAELTDGIRRLREGDAFYQVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGDLLQRKLMQTNQIKKMLRYPMFLISSVCVMFYILQTLIIPQFSGIYQSMNMNTSGATAFIFAFF
GHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPCYEKDLAAAVSHGQANGRLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=657584 MJE83_RS13600 WP_218791295.1 2713790..2714827(-) (comGB) [Bacillus velezensis strain R-71003]
ATGAAGCGGATTAAACGAACCTGGCCCTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACAGAAAAAGG
ATACAGTCTGATTGAAGGGCTCAGGCTGCTGAAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGACGGACGGAATCC
GCCGGCTGAGGGAAGGTGATGCGTTTTACCAAGTGCTTGAGGCACTGTCTTTTCACAAAGAAGCCGTCTCTATCTGCTAT
TTCGCGGAGAAACACGGGGAATTGCCGGGGGCGATGAAACAAAGCGGCGACTTGTTACAGAGAAAGCTTATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATATCTTCCGTCTGCGTCATGTTTTATATCCTGCAAACTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACATCCGGTGCGACTGCTTTCATCTTTGCATTTTTT
GGGCATTTTCATGAAGCCTGCGCACTGGCGCTGTCCGCCGCTTTCTGCTTGTTTTTGTATGTTTGGTTCTTATGTAAGAA
AAAATCCCCGCAAGATAAAATGCTGATTGTTGTGAAAATACCCCTCTTGGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTATCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTGTTATGAAAAAGACCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCGGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

65.635

93.623

0.614