Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   MJE83_RS13590 Genome accession   NZ_CP092446
Coordinates   2713050..2713487 (-) Length   145 a.a.
NCBI ID   WP_015240210.1    Uniprot ID   -
Organism   Bacillus velezensis strain R-71003     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2708050..2718487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJE83_RS13540 (MJE83_13540) sinI 2708434..2708607 (+) 174 WP_003153105.1 anti-repressor SinI family protein Regulator
  MJE83_RS13545 (MJE83_13545) sinR 2708641..2708976 (+) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  MJE83_RS13550 (MJE83_13550) - 2709024..2709809 (-) 786 WP_007408329.1 TasA family protein -
  MJE83_RS13555 (MJE83_13555) - 2709874..2710458 (-) 585 WP_015240205.1 signal peptidase I -
  MJE83_RS13560 (MJE83_13560) tapA 2710430..2711101 (-) 672 WP_124692843.1 amyloid fiber anchoring/assembly protein TapA -
  MJE83_RS13565 (MJE83_13565) - 2711360..2711689 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  MJE83_RS13570 (MJE83_13570) - 2711729..2711908 (-) 180 WP_003153093.1 YqzE family protein -
  MJE83_RS13575 (MJE83_13575) comGG 2711965..2712342 (-) 378 WP_015240208.1 competence type IV pilus minor pilin ComGG Machinery gene
  MJE83_RS13580 (MJE83_13580) comGF 2712343..2712843 (-) 501 WP_256994853.1 competence type IV pilus minor pilin ComGF -
  MJE83_RS13585 (MJE83_13585) comGE 2712752..2713066 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  MJE83_RS13590 (MJE83_13590) comGD 2713050..2713487 (-) 438 WP_015240210.1 competence type IV pilus minor pilin ComGD Machinery gene
  MJE83_RS13595 (MJE83_13595) comGC 2713477..2713785 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  MJE83_RS13600 (MJE83_13600) comGB 2713790..2714827 (-) 1038 WP_218791295.1 competence type IV pilus assembly protein ComGB Machinery gene
  MJE83_RS13605 (MJE83_13605) comGA 2714814..2715884 (-) 1071 WP_124692840.1 competence type IV pilus ATPase ComGA Machinery gene
  MJE83_RS13610 (MJE83_13610) - 2716077..2717027 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  MJE83_RS13615 (MJE83_13615) - 2717173..2718474 (+) 1302 WP_012117986.1 hemolysin family protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16272.75 Da        Isoelectric Point: 10.2186

>NTDB_id=657582 MJE83_RS13590 WP_015240210.1 2713050..2713487(-) (comGD) [Bacillus velezensis strain R-71003]
MNNNRRTENGFTLLESLIVLSLASVLLTVLFTTVPPAYTHLAVRQKTEQLQKDIQLAQETAIAEHKRTKITFLPKEHKYK
LQSGGKILERSFDSLHITLVTLPESLEFNEKGHPNSGGKIQLKSAGFTYEITVYLGSGNVHAKRK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=657582 MJE83_RS13590 WP_015240210.1 2713050..2713487(-) (comGD) [Bacillus velezensis strain R-71003]
TTGAACAATAACAGGCGGACAGAAAATGGGTTTACCCTTCTTGAAAGCCTGATTGTGTTAAGCCTGGCGTCCGTCCTGCT
GACGGTTTTGTTCACGACGGTTCCGCCGGCCTACACCCACCTTGCCGTGCGGCAAAAAACAGAGCAGCTCCAAAAAGATA
TTCAGCTTGCTCAGGAAACGGCTATCGCAGAACATAAAAGAACAAAAATCACTTTTCTTCCAAAAGAGCATAAATACAAA
CTGCAGTCAGGCGGAAAGATTCTCGAGCGTTCCTTTGATTCGCTTCACATTACACTTGTGACTTTACCGGAGAGCCTTGA
ATTTAACGAAAAAGGCCATCCGAATTCGGGAGGAAAGATTCAATTGAAGAGCGCCGGGTTCACCTATGAGATCACAGTTT
ACTTAGGGAGCGGAAATGTTCATGCTAAACGGAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Bacillus subtilis subsp. subtilis str. 168

56.849

100

0.572