Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MJE83_RS09120 Genome accession   NZ_CP092446
Coordinates   1840057..1840491 (+) Length   144 a.a.
NCBI ID   WP_015417516.1    Uniprot ID   -
Organism   Bacillus velezensis strain R-71003     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1835057..1845491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJE83_RS09115 (MJE83_09115) - 1838557..1839768 (-) 1212 WP_124693028.1 cytochrome P450 -
  MJE83_RS09120 (MJE83_09120) nucA/comI 1840057..1840491 (+) 435 WP_015417516.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MJE83_RS09125 (MJE83_09125) - 1840551..1841306 (+) 756 WP_003154084.1 YoaK family protein -
  MJE83_RS09130 (MJE83_09130) - 1841340..1841702 (-) 363 WP_007410383.1 hypothetical protein -
  MJE83_RS09135 (MJE83_09135) - 1841896..1843224 (-) 1329 WP_124693027.1 S8 family peptidase -
  MJE83_RS09140 (MJE83_09140) - 1843403..1843636 (+) 234 WP_015239897.1 hypothetical protein -
  MJE83_RS09145 (MJE83_09145) - 1843892..1844599 (+) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  MJE83_RS09150 (MJE83_09150) - 1844659..1845111 (+) 453 WP_012117605.1 OsmC family protein -
  MJE83_RS09155 (MJE83_09155) - 1845125..1845478 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15514.44 Da        Isoelectric Point: 7.2418

>NTDB_id=657575 MJE83_RS09120 WP_015417516.1 1840057..1840491(+) (nucA/comI) [Bacillus velezensis strain R-71003]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=657575 MJE83_RS09120 WP_015417516.1 1840057..1840491(+) (nucA/comI) [Bacillus velezensis strain R-71003]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGTATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.258

86.111

0.493