Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   MG974_RS17470 Genome accession   NZ_CP092439
Coordinates   3559523..3560668 (+) Length   381 a.a.
NCBI ID   WP_171463628.1    Uniprot ID   -
Organism   Bacillus velezensis strain 504     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3554523..3565668
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MG974_RS17450 (MG974_17450) - 3554997..3555713 (-) 717 WP_014306017.1 Crp/Fnr family transcriptional regulator -
  MG974_RS17455 (MG974_17455) narK 3555811..3557001 (-) 1191 WP_003151073.1 nitrate transporter NarK -
  MG974_RS17460 (MG974_17460) argS 3557136..3558806 (-) 1671 WP_061890879.1 arginine--tRNA ligase -
  MG974_RS17465 (MG974_17465) - 3558803..3559231 (-) 429 WP_003151069.1 DUF1934 domain-containing protein -
  MG974_RS17470 (MG974_17470) rapF 3559523..3560668 (+) 1146 WP_171463628.1 tetratricopeptide repeat protein Regulator
  MG974_RS17475 (MG974_17475) - 3560652..3560771 (+) 120 WP_015387559.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  MG974_RS17480 (MG974_17480) - 3560970..3561173 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  MG974_RS17485 (MG974_17485) - 3561187..3561390 (+) 204 WP_003151062.1 hypothetical protein -
  MG974_RS17495 (MG974_17495) - 3561797..3562078 (+) 282 WP_014306021.1 hypothetical protein -
  MG974_RS17500 (MG974_17500) - 3562122..3562634 (+) 513 WP_230639670.1 stage II sporulation protein M -
  MG974_RS17505 (MG974_17505) - 3563225..3563443 (-) 219 WP_003151056.1 helix-turn-helix transcriptional regulator -
  MG974_RS17510 (MG974_17510) speB 3563613..3564485 (-) 873 WP_003151054.1 agmatinase -
  MG974_RS17515 (MG974_17515) speE 3564545..3565375 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45569.76 Da        Isoelectric Point: 4.6057

>NTDB_id=657472 MG974_RS17470 WP_171463628.1 3559523..3560668(+) (rapF) [Bacillus velezensis strain 504]
MASVLSSSAIGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLVDIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQGNYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIVEYEFLESLYVGEPDEEAI
MECFDFLRDKMMYADLEDFALDVAKYYHERENFEKAAAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=657472 MG974_RS17470 WP_171463628.1 3559523..3560668(+) (rapF) [Bacillus velezensis strain 504]
GTGGCGAGTGTTTTATCGTCTTCAGCGATTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTATTTGCGAAGAGAAATCAAAGAAGAACTTGCAGGCCTGGAAGATGACCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGTGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTGGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAGCAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCTTT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGGAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATTATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAATATGAATTTTTAGAATCTTTGTATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAAATGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAGAATTTCGAGAAGGCTGCTGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(189-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.74

100

0.787
  rapC Bacillus subtilis subsp. subtilis str. 168

56.842

99.738

0.567