Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   MG974_RS11735 Genome accession   NZ_CP092439
Coordinates   2432871..2433908 (-) Length   345 a.a.
NCBI ID   WP_024085602.1    Uniprot ID   -
Organism   Bacillus velezensis strain 504     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2427871..2438908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MG974_RS11685 (MG974_11685) tasA 2428105..2428890 (-) 786 WP_003153102.1 biofilm matrix protein TasA -
  MG974_RS11690 (MG974_11690) sipW 2428954..2429538 (-) 585 WP_012117977.1 signal peptidase I SipW -
  MG974_RS11695 (MG974_11695) tapA 2429510..2430181 (-) 672 WP_024085598.1 amyloid fiber anchoring/assembly protein TapA -
  MG974_RS11700 (MG974_11700) - 2430441..2430770 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  MG974_RS11705 (MG974_11705) - 2430810..2430989 (-) 180 WP_003153093.1 YqzE family protein -
  MG974_RS11710 (MG974_11710) comGG 2431046..2431423 (-) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  MG974_RS11715 (MG974_11715) comGF 2431424..2431924 (-) 501 WP_224979425.1 competence type IV pilus minor pilin ComGF -
  MG974_RS11720 (MG974_11720) comGE 2431833..2432147 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  MG974_RS11725 (MG974_11725) comGD 2432131..2432568 (-) 438 WP_044053464.1 competence type IV pilus minor pilin ComGD Machinery gene
  MG974_RS11730 (MG974_11730) comGC 2432558..2432866 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  MG974_RS11735 (MG974_11735) comGB 2432871..2433908 (-) 1038 WP_024085602.1 competence type IV pilus assembly protein ComGB Machinery gene
  MG974_RS11740 (MG974_11740) comGA 2433895..2434965 (-) 1071 WP_044053465.1 competence type IV pilus ATPase ComGA Machinery gene
  MG974_RS11745 (MG974_11745) - 2435157..2436107 (-) 951 WP_014305415.1 magnesium transporter CorA family protein -
  MG974_RS11750 (MG974_11750) - 2436253..2437554 (+) 1302 WP_060657458.1 hemolysin family protein -
  MG974_RS11755 (MG974_11755) - 2437592..2438422 (-) 831 WP_003153080.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39618.91 Da        Isoelectric Point: 9.5658

>NTDB_id=657445 MG974_RS11735 WP_024085602.1 2432871..2433908(-) (comGB) [Bacillus velezensis strain 504]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLKLQLHKRQLAELTDGIRRLREGDAFYEVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGELLQRKLMQTNQIKKMLRYPMFLISSVCVMFYILQSLIIPQFSGIYQSMNMNTSGATAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPYYEKDLAAAVSHGQANGLLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=657445 MG974_RS11735 WP_024085602.1 2432871..2433908(-) (comGB) [Bacillus velezensis strain 504]
ATGAAGCGGATTAAACGAACCTGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGTCTGATTGAAGGGCTCAGACTGCTGAAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGACGGACGGAATCC
GCCGGCTGAGGGAAGGTGATGCGTTTTACGAAGTGCTTGAGGCACTGTCTTTTCATAAAGAAGCCGTCTCTATCTGCTAT
TTCGCAGAGAAACACGGAGAATTGCCGGGGGCGATGAAACAAAGCGGCGAATTATTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTCTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACATCCGGTGCGACTGCTTTCATCTTTGCATTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTGTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAATCCCCGCAAGATAAAATGCTCATTGTTGTGAAAATACCCCTCTTGGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTATTATGAAAAAGATCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCTGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCTAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

65.635

93.623

0.614