Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   TSP01S_RS01510 Genome accession   NZ_AP014509
Coordinates   308627..309508 (+) Length   293 a.a.
NCBI ID   WP_041075866.1    Uniprot ID   -
Organism   Thermotoga caldifontis AZM44c09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 303627..314508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TSP01S_RS01495 - 304382..304579 (+) 198 WP_041075860.1 hypothetical protein -
  TSP01S_RS01500 - 304566..306725 (+) 2160 WP_041075862.1 glycoside hydrolase family 3 protein -
  TSP01S_RS01505 - 306900..308624 (+) 1725 WP_041075864.1 proline--tRNA ligase -
  TSP01S_RS01510 pilA 308627..309508 (+) 882 WP_041075866.1 signal recognition particle-docking protein FtsY Machinery gene
  TSP01S_RS01515 - 309552..310208 (+) 657 WP_041075868.1 DUF2225 domain-containing protein -
  TSP01S_RS01520 - 310205..311920 (+) 1716 WP_041075870.1 peptidoglycan DD-metalloendopeptidase family protein -
  TSP01S_RS01525 - 311917..312342 (+) 426 WP_052463446.1 Rrf2 family transcriptional regulator -
  TSP01S_RS01530 - 312339..313295 (+) 957 WP_041075872.1 GAF domain-containing protein -
  TSP01S_RS01535 - 313292..314431 (+) 1140 WP_041075874.1 type III PLP-dependent enzyme -

Sequence

Protein


Download         Length: 293 a.a.        Molecular weight: 31788.76 Da        Isoelectric Point: 6.3990

>NTDB_id=65724 TSP01S_RS01510 WP_041075866.1 308627..309508(+) (pilA) [Thermotoga caldifontis AZM44c09]
MGLFERLKQGLEKTKKAFFEGIKQLLRSGRIDEETLEELEEILIAADVGHETTSWIIEKLKEQRVEDPVSALKEILIELL
EGDTSLNLTGSPSVISVVGVNGSGKTTTVAKLAAQFQSMGKSVVMAAADTFRAAAIEQLRVWGERINCTVIAHTEGSDPA
AVAYDAVNHAKSKGKDIVIIDTAGRLHTKKNLMEELRKIHRVVGKLVEGAPHEVLLVIDATTGQNGLMQARVFKEMVNVT
GLVITKLDGTAKGGIALAIKHQLSLPIKFIGVGESAEDLRPFDARQFVEALFS

Nucleotide


Download         Length: 882 bp        

>NTDB_id=65724 TSP01S_RS01510 WP_041075866.1 308627..309508(+) (pilA) [Thermotoga caldifontis AZM44c09]
GTGGGTCTGTTCGAGAGGTTGAAACAGGGGCTCGAAAAAACGAAGAAGGCTTTCTTCGAGGGAATAAAACAGCTTCTCAG
GAGCGGAAGAATAGACGAAGAAACACTGGAAGAACTCGAAGAGATACTCATCGCTGCCGACGTTGGCCACGAAACGACGA
GCTGGATCATCGAAAAACTCAAAGAACAGAGAGTGGAAGATCCTGTAAGCGCTTTGAAGGAGATACTGATCGAACTGCTC
GAAGGCGACACATCGCTGAACCTGACAGGTTCACCGAGCGTGATAAGCGTCGTGGGTGTGAACGGTTCTGGAAAGACCAC
GACGGTTGCGAAACTCGCCGCACAGTTTCAGTCGATGGGCAAGAGCGTGGTGATGGCCGCTGCAGACACGTTCAGGGCCG
CCGCGATCGAGCAACTCAGAGTGTGGGGAGAAAGGATCAACTGCACGGTGATCGCGCACACAGAAGGTTCAGATCCCGCG
GCTGTGGCGTACGATGCGGTGAACCACGCGAAGTCGAAGGGCAAAGACATCGTGATCATCGACACGGCAGGAAGGCTCCA
CACAAAGAAGAACCTCATGGAAGAGCTCAGAAAGATCCACAGGGTCGTTGGAAAGCTCGTAGAAGGTGCACCACACGAAG
TTCTACTCGTGATAGACGCGACGACGGGACAGAACGGCCTGATGCAGGCGCGCGTCTTCAAAGAAATGGTGAACGTGACC
GGTCTTGTCATCACCAAGCTCGACGGAACCGCCAAGGGTGGTATCGCCCTGGCGATAAAACACCAGCTTTCTTTACCGAT
CAAATTCATCGGTGTTGGAGAAAGCGCTGAAGATCTGAGACCTTTCGATGCGCGACAGTTCGTCGAGGCGTTGTTCTCTT
GA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(20-71)

SRP54-type protein, GTPase domain (SRP54)

(92-292)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.515

100

0.502

Multiple sequence alignment