Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MGJ28_RS20295 Genome accession   NZ_CP092387
Coordinates   3844505..3844879 (+) Length   124 a.a.
NCBI ID   WP_223269005.1    Uniprot ID   -
Organism   Priestia megaterium strain BP01R2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3839505..3849879
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MGJ28_RS20275 (MGJ28_20280) - 3839998..3840669 (-) 672 WP_016765173.1 response regulator transcription factor -
  MGJ28_RS20280 (MGJ28_20285) - 3840995..3841813 (+) 819 WP_013058559.1 squalene/phytoene synthase family protein -
  MGJ28_RS20285 (MGJ28_20290) - 3841849..3843798 (-) 1950 WP_016765174.1 FUSC family protein -
  MGJ28_RS20290 (MGJ28_20295) - 3843955..3844215 (-) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  MGJ28_RS20295 (MGJ28_20300) nucA/comI 3844505..3844879 (+) 375 WP_223269005.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MGJ28_RS20300 (MGJ28_20305) comJ 3844915..3845322 (+) 408 WP_016765176.1 competence protein ComJ -
  MGJ28_RS20305 (MGJ28_20310) helD 3845351..3847672 (-) 2322 WP_016765177.1 RNA polymerase recycling motor HelD -
  MGJ28_RS29430 - 3847917..3848042 (-) 126 WP_016765178.1 hypothetical protein -
  MGJ28_RS20310 (MGJ28_20315) - 3848182..3848697 (-) 516 WP_016765179.1 enoyl-CoA hydratase/isomerase family protein -
  MGJ28_RS20315 (MGJ28_20320) - 3848940..3849308 (+) 369 WP_016765180.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 124 a.a.        Molecular weight: 13661.90 Da        Isoelectric Point: 4.2601

>NTDB_id=657150 MGJ28_RS20295 WP_223269005.1 3844505..3844879(+) (nucA/comI) [Priestia megaterium strain BP01R2]
MGHAAFLAPEKTEAEVNYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEANREESLKGYPTKTGYDRDEWPMA
MCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 375 bp        

>NTDB_id=657150 MGJ28_RS20295 WP_223269005.1 3844505..3844879(+) (nucA/comI) [Priestia megaterium strain BP01R2]
ATGGGACATGCAGCTTTTTTAGCTCCTGAAAAAACGGAAGCTGAAGTAAATTATGATGCGACACTTTATTTTCCGTTAGA
CCGATATCCTGAAACGGGTGATCATATTCGTGACGCGATTGCCGCTGGGCACTCTTCCGTTTGCACAATTGATCGCGACG
GTGCTGAAGCGAACCGAGAAGAATCCCTGAAAGGATATCCGACAAAAACAGGTTATGACCGTGATGAATGGCCAATGGCT
ATGTGCGAAGAAGGCGGAGCAGGAGCTGACATTCGCTATATTTCACCTTCTGATAACCGTGGAGCCGGCTCATGGGTTGG
AAATCAGTTAGAACAGTATCCAGATGGTACACGCGTACAATTTATCGTTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(30-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

67.227

95.968

0.645