Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MGJ28_RS17480 Genome accession   NZ_CP092387
Coordinates   3364888..3365310 (+) Length   140 a.a.
NCBI ID   WP_013083984.1    Uniprot ID   A0A1Q8UP88
Organism   Priestia megaterium strain BP01R2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3359888..3370310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MGJ28_RS17455 (MGJ28_17460) - 3360219..3361052 (-) 834 WP_016764866.1 AraC family ligand binding domain-containing protein -
  MGJ28_RS17460 (MGJ28_17465) - 3361276..3362097 (+) 822 WP_016764867.1 sensor histidine kinase -
  MGJ28_RS17465 (MGJ28_17470) - 3362098..3362739 (+) 642 WP_241096353.1 response regulator -
  MGJ28_RS17470 (MGJ28_17475) - 3362871..3364253 (+) 1383 WP_016764869.1 PepSY-associated TM helix domain-containing protein -
  MGJ28_RS17475 (MGJ28_17480) - 3364283..3364747 (+) 465 WP_016764870.1 FixH family protein -
  MGJ28_RS17480 (MGJ28_17485) nucA/comI 3364888..3365310 (+) 423 WP_013083984.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MGJ28_RS17485 (MGJ28_17490) - 3365465..3365818 (+) 354 WP_241096354.1 DUF1516 family protein -
  MGJ28_RS17490 (MGJ28_17495) - 3365859..3366452 (-) 594 WP_016764872.1 hypothetical protein -
  MGJ28_RS17495 (MGJ28_17500) - 3366607..3367314 (-) 708 WP_016764873.1 TetR/AcrR family transcriptional regulator -
  MGJ28_RS17500 (MGJ28_17505) - 3367286..3368566 (-) 1281 WP_241096355.1 glycosyltransferase -
  MGJ28_RS17505 (MGJ28_17510) - 3368861..3369406 (+) 546 WP_034650492.1 GNAT family N-acetyltransferase -
  MGJ28_RS17510 (MGJ28_17515) - 3369705..3370190 (-) 486 WP_241096356.1 hypothetical protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15356.20 Da        Isoelectric Point: 4.4990

>NTDB_id=657147 MGJ28_RS17480 WP_013083984.1 3364888..3365310(+) (nucA/comI) [Priestia megaterium strain BP01R2]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGNGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=657147 MGJ28_RS17480 WP_013083984.1 3364888..3365310(+) (nucA/comI) [Priestia megaterium strain BP01R2]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATCGACCGAGACGGTGCTGAAGAAAACCGCCGTGAGTCTTTAAAG
GGCATTCCGACTAAAAGCGGCTACGATCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAACGGTGCAGACAT
TGAATATGTAAAGCCTTCAGACAACCGAGGCGCCGGATCTTGGATCTCACATCAAGTAGACGAGTTTCCAGACGGCACGA
AGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q8UP88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507