Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   MID02_RS18365 Genome accession   NZ_CP092383
Coordinates   3731837..3732982 (+) Length   381 a.a.
NCBI ID   WP_239819300.1    Uniprot ID   -
Organism   Bacillus velezensis strain SF327     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3726837..3737982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MID02_RS18345 (MID02_18345) - 3727311..3728027 (-) 717 WP_014306017.1 Crp/Fnr family transcriptional regulator -
  MID02_RS18350 (MID02_18350) narK 3728125..3729315 (-) 1191 WP_044052814.1 nitrate transporter NarK -
  MID02_RS18355 (MID02_18355) argS 3729450..3731120 (-) 1671 WP_025853601.1 arginine--tRNA ligase -
  MID02_RS18360 (MID02_18360) - 3731117..3731545 (-) 429 WP_003151069.1 DUF1934 family protein -
  MID02_RS18365 (MID02_18365) rapF 3731837..3732982 (+) 1146 WP_239819300.1 tetratricopeptide repeat protein Regulator
  MID02_RS18370 (MID02_18370) - 3732966..3733085 (+) 120 WP_003151066.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  MID02_RS18375 (MID02_18375) - 3733284..3733487 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  MID02_RS18380 (MID02_18380) - 3733501..3733704 (+) 204 WP_003151062.1 hypothetical protein -
  MID02_RS20235 - 3733739..3733925 (+) 187 Protein_3577 DUF4177 domain-containing protein -
  MID02_RS18385 (MID02_18385) - 3734113..3734394 (+) 282 WP_014306021.1 hypothetical protein -
  MID02_RS18390 (MID02_18390) - 3734438..3734950 (+) 513 WP_230639670.1 stage II sporulation protein M -
  MID02_RS18395 (MID02_18395) - 3735541..3735759 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  MID02_RS18400 (MID02_18400) speB 3735929..3736801 (-) 873 WP_058906421.1 agmatinase -
  MID02_RS18405 (MID02_18405) speE 3736861..3737691 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45367.54 Da        Isoelectric Point: 4.9264

>NTDB_id=657068 MID02_RS18365 WP_239819300.1 3731837..3732982(+) (rapF) [Bacillus velezensis strain SF327]
MASVLSSSAIGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLVDIDKKQARLTGRLDYYFNFFRGMYELDQREYLSAIKFFKKAETKLSFIKDTIDKAEYFFKMSEVYYYLKQTYVS
TDYARQAYEIYKAQSSYDIRLIQCQSLFGANFLDLKLYDRAITHFQKAFSLAETEKQPQLMGRTLYNIALCKQSLNDYEA
AITYVEKAIDVFESAKMTPSLPQAYFLMTQIHYKLERYDKALEYHKKGVIYSEESADSVYLHEFEFLKSLYVNGPDEDAM
KRSFDFLESKMMYADIEDFALDAAKYYHKLEDYKKASTYFLKVEEARQHIQGGVTFYEAEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=657068 MID02_RS18365 WP_239819300.1 3731837..3732982(+) (rapF) [Bacillus velezensis strain SF327]
GTGGCGAGTGTTTTATCGTCTTCAGCGATTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTATTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGCCTGGAAGATGACCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGTGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGATCAGCGGGAATATTTGTCGGCTATAAAATTTTTTAAAAAGGCTGAGACGAAACTCTCATTTA
TTAAGGATACTATTGATAAAGCCGAGTACTTTTTCAAGATGTCTGAAGTCTACTACTATTTGAAACAAACGTATGTATCT
ACCGATTACGCAAGGCAGGCATATGAAATATATAAAGCTCAGTCGTCATACGATATCAGGCTCATACAATGCCAATCCTT
ATTCGGAGCAAATTTCCTGGATTTAAAACTATATGATCGGGCAATTACTCACTTTCAAAAGGCTTTTTCTTTGGCAGAAA
CAGAAAAACAGCCTCAATTAATGGGCAGGACGCTGTATAACATCGCACTTTGCAAACAAAGCCTGAACGACTATGAAGCA
GCTATAACTTATGTTGAAAAAGCCATTGATGTTTTTGAAAGTGCTAAAATGACCCCTTCTTTGCCCCAAGCATATTTTTT
AATGACACAGATTCATTATAAATTAGAAAGATACGACAAAGCGCTCGAGTATCATAAAAAAGGAGTTATCTATTCCGAAG
AGTCAGCAGATTCAGTTTATTTACACGAATTCGAATTTTTAAAATCTTTGTATGTGAACGGACCTGATGAGGATGCAATG
AAACGGAGTTTTGACTTTTTGGAAAGTAAGATGATGTATGCAGATATAGAAGATTTTGCTCTTGATGCAGCTAAATATTA
TCACAAACTTGAAGATTATAAAAAAGCTTCAACATACTTTTTAAAAGTTGAAGAAGCAAGACAGCATATTCAAGGGGGGG
TAACTTTTTATGAAGCTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(223-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

74.803

100

0.748
  rapC Bacillus subtilis subsp. subtilis str. 168

52.895

99.738

0.528