Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MID02_RS01765 Genome accession   NZ_CP092383
Coordinates   323994..324431 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain SF327     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 318994..329431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MID02_RS01725 (MID02_01725) - 319574..319735 (-) 162 Protein_299 hypothetical protein -
  MID02_RS01730 (MID02_01730) - 319852..321054 (+) 1203 WP_069473143.1 GTP-binding protein -
  MID02_RS01735 (MID02_01735) - 321119..322255 (+) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  MID02_RS01740 (MID02_01740) - 322270..322704 (+) 435 WP_003156592.1 RDD family protein -
  MID02_RS01745 (MID02_01745) - 322776..323096 (+) 321 WP_015388719.1 YckD family protein -
  MID02_RS01755 (MID02_01755) - 323315..323533 (+) 219 Protein_304 family 1 glycosylhydrolase -
  MID02_RS01760 (MID02_01760) nin/comJ 323574..323972 (-) 399 WP_003156589.1 competence protein ComJ Regulator
  MID02_RS01765 (MID02_01765) nucA/comI 323994..324431 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MID02_RS01770 (MID02_01770) hxlB 324790..325347 (-) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  MID02_RS01775 (MID02_01775) hxlA 325344..325979 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  MID02_RS01780 (MID02_01780) - 326211..326573 (+) 363 WP_014304320.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=657003 MID02_RS01765 WP_003156588.1 323994..324431(-) (nucA/comI) [Bacillus velezensis strain SF327]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=657003 MID02_RS01765 WP_003156588.1 323994..324431(-) (nucA/comI) [Bacillus velezensis strain SF327]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641