Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MIS45_RS07550 Genome accession   NZ_CP092361
Coordinates   1531972..1533354 (-) Length   460 a.a.
NCBI ID   WP_249450081.1    Uniprot ID   -
Organism   Wielerella bovis strain CCUG 44465     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1526972..1538354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS45_RS07505 (MIS45_07505) - 1527390..1527839 (+) 450 WP_249450075.1 DMP19 family protein -
  MIS45_RS07510 (MIS45_07510) truA 1527849..1528634 (+) 786 WP_249449616.1 tRNA pseudouridine(38-40) synthase TruA -
  MIS45_RS07515 (MIS45_07515) - 1529120..1529389 (-) 270 WP_249450076.1 hypothetical protein -
  MIS45_RS07520 (MIS45_07520) - 1529591..1529971 (-) 381 WP_249450077.1 hypothetical protein -
  MIS45_RS07525 (MIS45_07525) - 1530079..1530288 (-) 210 WP_249450078.1 hypothetical protein -
  MIS45_RS07530 (MIS45_07530) - 1530352..1530654 (+) 303 WP_249441922.1 IS630 transposase-related protein -
  MIS45_RS07535 (MIS45_07535) - 1530680..1531189 (+) 510 WP_249447679.1 IS630 family transposase -
  MIS45_RS07540 (MIS45_07540) - 1531164..1531694 (-) 531 WP_249450079.1 P-loop NTPase fold protein -
  MIS45_RS07545 (MIS45_07545) - 1531737..1531979 (-) 243 WP_249450080.1 hypothetical protein -
  MIS45_RS07550 (MIS45_07550) radA 1531972..1533354 (-) 1383 WP_249450081.1 DNA repair protein RadA Machinery gene
  MIS45_RS07555 (MIS45_07555) - 1533528..1534142 (-) 615 WP_249450082.1 hypothetical protein -
  MIS45_RS07560 (MIS45_07560) - 1534617..1535834 (+) 1218 WP_249448179.1 sugar MFS transporter -
  MIS45_RS07565 (MIS45_07565) - 1535970..1536245 (-) 276 WP_249450083.1 hypothetical protein -
  MIS45_RS07570 (MIS45_07570) hflX 1536363..1537499 (+) 1137 WP_249450084.1 GTPase HflX -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49559.27 Da        Isoelectric Point: 7.1114

>NTDB_id=656777 MIS45_RS07550 WP_249450081.1 1531972..1533354(-) (radA) [Wielerella bovis strain CCUG 44465]
MAKAPKTVYQCTECGGTSPKWVGKCPHCGEWNTLQENIAASEPKNARFQSWAAETTQVQALSQVTAMEVPREATGMGELD
RVLGGGLVDGAVILLGGDPGIGKSTLLLQTIALMAKKRKVLYISGEESAQQVALRAQRLGLNAEGVNLLAEIRLEAIQAA
LKQHAPSVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRIAKQMGIAMIFVGHVTKDGAIAGPRVLEHMVDTVLYFEG
DQHSNHRMIRAIKNRFGAANELGVFAMTETGLKGVSNPSAIFLASYRDDVAGSCVLVTQEGTRPLLVEIQSLVDDAHGFT
PKRLTVGLEQNRLAMLLAVLNRHAGIACFDQDVFLNAVGGVKIGEPATDLAAVLAMVSSFRNKPLPEKMVAFGEIGLSGE
IRPVPRGQERLKEAEKLGFKRAIVPKANLPRNENEFPKLKIYGVESLQEAVMLCRDLVDE

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=656777 MIS45_RS07550 WP_249450081.1 1531972..1533354(-) (radA) [Wielerella bovis strain CCUG 44465]
ATGGCAAAAGCCCCCAAAACCGTCTATCAATGCACCGAATGCGGCGGCACATCGCCCAAATGGGTTGGCAAATGCCCCCA
TTGTGGCGAATGGAACACGCTACAAGAAAACATCGCCGCCTCCGAACCGAAAAATGCCCGTTTCCAATCATGGGCAGCCG
AAACGACACAAGTCCAAGCCCTATCCCAAGTAACCGCCATGGAAGTACCACGCGAAGCCACAGGCATGGGCGAATTAGAC
CGCGTTTTGGGCGGCGGTTTGGTGGACGGTGCCGTGATTTTATTGGGTGGCGACCCAGGGATTGGCAAATCCACATTACT
CTTACAAACCATTGCTTTAATGGCGAAAAAACGCAAGGTCTTGTACATTTCAGGCGAAGAATCCGCGCAACAAGTTGCCC
TACGCGCCCAGCGTTTAGGTTTGAACGCAGAAGGCGTGAATTTGCTTGCCGAAATCCGTTTGGAAGCCATTCAGGCTGCC
TTAAAACAACACGCGCCCAGCGTGGTTGTGATTGACTCCATTCAAACCATGTATTCTGACCAAATCACGTCCGCCCCAGG
CTCCGTGTCGCAAGTGCGCGAATGTGCCGCCCAACTCACGCGCATCGCCAAACAAATGGGCATCGCCATGATTTTTGTCG
GACACGTTACCAAAGACGGCGCCATCGCAGGCCCGCGCGTGTTGGAACACATGGTGGATACCGTGTTGTATTTTGAAGGC
GACCAGCATTCCAATCATCGCATGATACGCGCGATTAAAAACCGCTTTGGCGCGGCAAACGAATTGGGCGTGTTTGCCAT
GACCGAAACGGGTTTGAAAGGCGTATCCAATCCGTCCGCGATTTTTTTGGCGAGCTATCGGGACGATGTGGCGGGTTCGT
GCGTCTTGGTAACGCAGGAAGGAACGCGCCCATTATTGGTGGAAATTCAATCACTTGTAGATGATGCACACGGTTTCACG
CCCAAACGCTTAACGGTCGGTTTGGAACAAAACCGTTTGGCGATGTTGCTGGCGGTGTTGAACCGTCATGCGGGCATTGC
CTGTTTTGACCAAGATGTGTTTTTGAACGCGGTGGGTGGCGTGAAAATTGGCGAACCTGCGACGGATTTGGCGGCGGTAT
TGGCGATGGTTTCCAGTTTCAGAAACAAGCCTTTGCCTGAAAAAATGGTGGCGTTTGGCGAAATCGGTTTGAGTGGCGAA
ATCCGCCCCGTGCCGCGCGGTCAGGAGCGTTTGAAAGAAGCGGAAAAATTGGGTTTCAAACGCGCCATTGTGCCGAAAGC
GAATTTGCCGCGCAATGAAAACGAGTTCCCCAAATTAAAAATTTATGGCGTGGAAAGTTTGCAAGAAGCGGTTATGTTGT
GTCGGGATTTGGTTGATGAATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(366-428)

AAA domain (AAA_25)

(80-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

51.316

99.13

0.509
  radA Streptococcus pneumoniae Rx1

48.23

98.261

0.474
  radA Streptococcus pneumoniae D39

48.23

98.261

0.474
  radA Streptococcus pneumoniae R6

48.23

98.261

0.474
  radA Streptococcus pneumoniae TIGR4

48.23

98.261

0.474
  radA Streptococcus mitis SK321

47.912

98.913

0.474
  radA Streptococcus mitis NCTC 12261

47.692

98.913

0.472