Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MD222_RS02965 Genome accession   NZ_CP092264
Coordinates   638479..639855 (+) Length   458 a.a.
NCBI ID   WP_024855144.1    Uniprot ID   -
Organism   Levilactobacillus brevis strain YSJ3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 633479..644855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MD222_RS02935 (MD222_02935) - 633511..634743 (+) 1233 WP_011667342.1 serine hydrolase domain-containing protein -
  MD222_RS02940 (MD222_02940) - 634877..636199 (-) 1323 WP_369707378.1 C1 family peptidase -
  MD222_RS02945 (MD222_02945) - 636357..636527 (+) 171 WP_011667344.1 hypothetical protein -
  MD222_RS02950 (MD222_02950) rpiA 636631..637317 (-) 687 WP_011667345.1 ribose-5-phosphate isomerase RpiA -
  MD222_RS02955 (MD222_02955) - 637325..637666 (-) 342 WP_011667346.1 hypothetical protein -
  MD222_RS02960 (MD222_02960) - 637845..638384 (+) 540 WP_011667347.1 dUTP diphosphatase -
  MD222_RS02965 (MD222_02965) radA 638479..639855 (+) 1377 WP_024855144.1 DNA repair protein RadA Machinery gene
  MD222_RS02970 (MD222_02970) - 639891..641075 (+) 1185 WP_011667349.1 PIN/TRAM domain-containing protein -
  MD222_RS02975 (MD222_02975) gltX 641332..642822 (+) 1491 WP_011667350.1 glutamate--tRNA ligase -
  MD222_RS02980 (MD222_02980) cysS 642977..644389 (+) 1413 WP_011667351.1 cysteine--tRNA ligase -
  MD222_RS02985 (MD222_02985) - 644389..644796 (+) 408 WP_011667352.1 Mini-ribonuclease 3 -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49816.15 Da        Isoelectric Point: 7.0881

>NTDB_id=656384 MD222_RS02965 WP_024855144.1 638479..639855(+) (radA) [Levilactobacillus brevis strain YSJ3]
MAKPRTTYVCQNCDYSSPRYLGRCPNCGEWNTMVEEVVTPAAAKPQSTRTTASGERSHPQLMSEIKHSTESRTKTQMEEL
NRVLGGGIVPGSLILIGGDPGIGKSTLLLQVSGQLSQTGGKVLYVSGEESASQIKMRADRLVVNSDHLYLYPETDMASIR
ANIEQMKPDYVVIDSVQTMQAPGIESAIGSVSQIRAVTGELMQIAKTNGITIFVVGHVTKGGAIAGPKILEHMVDTVLYF
EGDLHHTYRILRAVKNRFGSTNELGIFEMREGGLYEVANPSEIFLEERLKDATGSAIVVSMEGTRPILVEVQALITPSVF
GNAQRTSSGLDRNRVSLLMAVLEKRANLMLQNQDAFLKAAGGVKLDEPAIDLAIALSIVSSYRDTATQPTDCFVGEVGLT
GEIRRVNRIEQRVAEAKKLGFKRIFIPKNNLQGWTPDPGIDVVGVSTLRQTLKLALDV

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=656384 MD222_RS02965 WP_024855144.1 638479..639855(+) (radA) [Levilactobacillus brevis strain YSJ3]
GTGGCGAAACCAAGAACAACGTATGTGTGTCAAAATTGTGATTATAGCTCACCCCGTTATTTGGGGCGTTGTCCCAATTG
TGGTGAGTGGAACACGATGGTGGAAGAAGTCGTCACACCGGCTGCCGCCAAGCCACAATCAACCCGGACCACTGCTTCTG
GAGAACGGTCACACCCCCAACTCATGAGTGAGATTAAACACTCAACGGAAAGCCGAACGAAGACGCAAATGGAAGAACTG
AACCGAGTGCTGGGTGGGGGGATTGTGCCGGGATCTTTGATCCTAATCGGTGGTGATCCTGGAATTGGAAAATCAACCCT
CTTGCTTCAAGTATCGGGGCAACTCAGCCAAACAGGCGGTAAAGTGTTGTATGTTTCTGGAGAAGAAAGTGCGTCACAGA
TTAAAATGCGGGCGGATCGGTTAGTCGTCAATAGCGACCACTTGTATCTTTATCCAGAAACTGATATGGCCAGTATTCGC
GCGAACATTGAACAGATGAAACCTGATTACGTGGTCATTGATTCCGTTCAGACCATGCAGGCGCCGGGAATCGAGTCGGC
GATTGGCTCGGTCTCACAAATTCGTGCGGTCACGGGTGAGCTGATGCAAATTGCCAAAACCAATGGCATCACTATTTTTG
TGGTGGGGCATGTGACGAAGGGCGGCGCAATTGCTGGTCCTAAGATTCTGGAACACATGGTGGATACGGTTCTTTACTTT
GAAGGTGACCTCCATCATACGTACCGAATTCTCCGAGCGGTCAAGAACCGATTTGGGTCAACGAATGAATTGGGTATCTT
TGAAATGCGTGAAGGTGGCTTGTATGAAGTTGCTAATCCGTCAGAAATTTTCTTGGAAGAACGATTGAAGGACGCAACCG
GCTCGGCCATTGTGGTATCAATGGAAGGTACGCGGCCAATTTTGGTAGAAGTTCAGGCGTTAATTACACCATCGGTCTTT
GGTAATGCGCAACGCACATCCAGCGGCTTGGATCGTAATCGGGTGTCGTTGTTGATGGCTGTGCTAGAAAAACGGGCCAA
TCTAATGCTCCAAAATCAAGATGCATTCTTAAAAGCCGCGGGAGGCGTTAAGTTAGATGAACCGGCGATTGACTTGGCGA
TCGCCCTGTCAATTGTCTCAAGTTATCGTGATACCGCTACGCAACCCACGGATTGTTTTGTCGGTGAAGTTGGCCTGACT
GGTGAAATCCGTCGGGTTAATCGAATTGAACAACGGGTTGCGGAAGCGAAAAAGCTGGGCTTTAAGCGGATCTTCATTCC
CAAGAATAACCTTCAAGGGTGGACGCCCGATCCCGGAATTGATGTGGTCGGCGTGTCAACGTTACGACAAACGTTAAAAC
TGGCTTTGGACGTGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

AAA domain (AAA_25)

(64-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

66.446

98.908

0.657
  radA Streptococcus mitis NCTC 12261

66.225

98.908

0.655
  radA Streptococcus pneumoniae Rx1

66.225

98.908

0.655
  radA Streptococcus pneumoniae D39

66.225

98.908

0.655
  radA Streptococcus pneumoniae R6

66.225

98.908

0.655
  radA Streptococcus pneumoniae TIGR4

66.225

98.908

0.655
  radA Bacillus subtilis subsp. subtilis str. 168

63.816

99.563

0.635