Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MF598_RS19570 Genome accession   NZ_CP092185
Coordinates   3926913..3928292 (-) Length   459 a.a.
NCBI ID   WP_014416742.1    Uniprot ID   I2C0L1
Organism   Bacillus velezensis strain K203     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3921913..3933292
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MF598_RS19550 (MF598_19550) ispF 3923433..3923909 (-) 477 WP_003156407.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  MF598_RS19555 (MF598_19555) ispD 3923902..3924600 (-) 699 WP_015239038.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MF598_RS19560 (MF598_19560) - 3924613..3925713 (-) 1101 WP_003156403.1 PIN/TRAM domain-containing protein -
  MF598_RS19565 (MF598_19565) disA 3925827..3926909 (-) 1083 WP_007410390.1 DNA integrity scanning diadenylate cyclase DisA -
  MF598_RS19570 (MF598_19570) radA 3926913..3928292 (-) 1380 WP_014416742.1 DNA repair protein RadA Machinery gene
  MF598_RS19575 (MF598_19575) clpC 3928386..3930818 (-) 2433 WP_015239036.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  MF598_RS19580 (MF598_19580) - 3930815..3931906 (-) 1092 WP_003156398.1 protein arginine kinase -
  MF598_RS19585 (MF598_19585) - 3931906..3932463 (-) 558 WP_007410387.1 UvrB/UvrC motif-containing protein -
  MF598_RS19590 (MF598_19590) ctsR 3932477..3932941 (-) 465 WP_003156396.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49547.98 Da        Isoelectric Point: 7.1760

>NTDB_id=656288 MF598_RS19570 WP_014416742.1 3926913..3928292(-) (radA) [Bacillus velezensis strain K203]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVHTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQMLHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPEANADGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=656288 MF598_RS19570 WP_014416742.1 3926913..3928292(-) (radA) [Bacillus velezensis strain K203]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAGTGGATGGGAAAGTGCCCGGGTTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATAAAAAAAGCGCCGGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACATCGGAAGAACCCCGCGTTCACACAAAGCTTGAAGAATTC
AACAGAGTGCTTGGCGGCGGCGTTGTGAAAGGCTCGTTAGTATTAATCGGCGGCGATCCCGGCATCGGAAAATCAACGCT
CCTTCTCCAAGTATCCGCACAATTAGCAGATACGGCCGGCAGCGTGCTTTACATTTCAGGGGAAGAATCCGTGAAGCAAA
CGAAGCTGCGCGCCGACAGACTTGGAATCAACAGCCAAATGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTCGTAGACTCCATTCAGACCGTTTACCAAAGTGATATCACCTCAGC
TCCCGGCAGTGTATCACAAGTAAGAGAGTGTACGGCCGAATTGATGAAAATCGCGAAAACAAACGGCATACCTATTTTTA
TCGTCGGGCACGTCACAAAAGAAGGATCGATCGCAGGGCCGAGATTGCTTGAGCACATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCACCATACATTCCGTATTTTGCGGGCCGTAAAAAACCGTTTCGGCTCCACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAGGGGCTTACGGAAGTGCTGAACCCTTCTGAAATTTTCTTGGAAGAGCGTTCTGCCGGGGCATCGG
GTTCGAGTATCGTTGCGTCAATGGAAGGAACGAGACCGATCCTGGTTGAAATCCAGGCGCTCATATCACCGACCAGTTTT
GGAAACCCGCGCCGCATGGCTACAGGCATTGACCACAACAGGGTATCGTTAATTATGGCTGTGCTTGAGAAGCGGGTGGG
TCTTTTGCTTCAAAATCAAGATGCGTATTTAAAGGTCGCCGGCGGGGTCAAACTGGATGAACCGGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCCTCAAGCTTCAGAGATACACCGCCGAATCCCGCAGATTGCTTTATCGGTGAAGTGGGTCTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAAGAAGCGGCAAAACTCGGCTTCAAGCGCATGATTATACC
TGAGGCGAATGCAGACGGGTGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

AAA domain (AAA_25)

(73-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C0L1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

93.45

99.782

0.932
  radA Streptococcus pneumoniae Rx1

63.797

98.693

0.63
  radA Streptococcus pneumoniae D39

63.797

98.693

0.63
  radA Streptococcus pneumoniae R6

63.797

98.693

0.63
  radA Streptococcus pneumoniae TIGR4

63.797

98.693

0.63
  radA Streptococcus mitis NCTC 12261

63.797

98.693

0.63
  radA Streptococcus mitis SK321

63.576

98.693

0.627