Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MF133_RS10590 Genome accession   NZ_CP092181
Coordinates   2317343..2318782 (-) Length   479 a.a.
NCBI ID   WP_041211850.1    Uniprot ID   A0A2L0TRY9
Organism   Aeromonas caviae strain 211703     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2312343..2323782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MF133_RS10560 (MF133_10560) - 2312622..2313362 (+) 741 WP_113978331.1 KDGP aldolase family protein -
  MF133_RS10565 (MF133_10565) - 2313466..2315370 (+) 1905 WP_239054154.1 HTH domain-containing protein -
  MF133_RS10570 (MF133_10570) npr 2315386..2315658 (-) 273 WP_201888080.1 PTS phosphocarrier protein NPr -
  MF133_RS10575 (MF133_10575) rapZ 2315679..2316545 (-) 867 WP_010675869.1 RNase adapter RapZ -
  MF133_RS10580 (MF133_10580) ptsN 2316586..2317032 (-) 447 WP_010675870.1 PTS IIA-like nitrogen regulatory protein PtsN -
  MF133_RS10585 (MF133_10585) hpf 2317035..2317322 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  MF133_RS10590 (MF133_10590) rpoN/rpoN1 2317343..2318782 (-) 1440 WP_041211850.1 RNA polymerase factor sigma-54 Machinery gene
  MF133_RS10595 (MF133_10595) lptB 2318848..2319573 (-) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  MF133_RS10600 (MF133_10600) lptA 2319586..2320104 (-) 519 WP_010675873.1 lipopolysaccharide transport periplasmic protein LptA -
  MF133_RS10605 (MF133_10605) lptC 2320091..2320651 (-) 561 WP_239054155.1 LPS export ABC transporter periplasmic protein LptC -
  MF133_RS10610 (MF133_10610) kdsC 2320648..2321202 (-) 555 WP_239054156.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  MF133_RS10615 (MF133_10615) - 2321202..2322197 (-) 996 WP_239054157.1 KpsF/GutQ family sugar-phosphate isomerase -
  MF133_RS10620 (MF133_10620) mlaF 2322404..2323204 (+) 801 WP_029314846.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53731.54 Da        Isoelectric Point: 4.3728

>NTDB_id=656163 MF133_RS10590 WP_041211850.1 2317343..2318782(-) (rpoN/rpoN1) [Aeromonas caviae strain 211703]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAQETSQAETQDASQLDSSDAMAQESMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTATLED
IQAAVSSEEVEVELDEIEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLDEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=656163 MF133_RS10590 WP_041211850.1 2317343..2318782(-) (rpoN/rpoN1) [Aeromonas caviae strain 211703]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAGAACGAGG
CGCAGGAAACCAGTCAGGCAGAGACACAGGACGCCAGTCAACTCGACTCCAGCGATGCCATGGCCCAGGAGAGCATGCCG
GATGAACTGCCGGTGGACACCACCTGGGACGAGGTCTACTCGGCCGGTACGGCCCAGAGTGCAGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAGACCACCGAGAACCTGCAGGACTACCTGCTGTGGCAGATGCGGCTCACCCCGTTCA
GTGATGTGGATGCTGCCATCGCCCTTGCCATCATCGATGCCATCGACGAATCCGGGTATCTGACTGCCACCCTCGAGGAT
ATTCAGGCTGCAGTCAGCAGCGAGGAGGTAGAAGTCGAACTGGATGAGATCGAAGCCGTGCTCAAACGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCCCGTTCGGTGCAAGAGTGTCTGCTGATCCAGCTCGGCCAGTATTCGCCGGACCTGCCCT
GGCTCGACGAAGCCAGAGAGGTGATCCGCGAACACATGGACCTGCTCGGCAATCGCGACTACCGCACCCTGGCTCGCAAG
ACCCGCCTCAAGGAGAGCGATCTCAAGGAGGTGCTGGATCTCATCCAGCAACTGGAGCCCAGACCCGGCAACGGCATCAT
CCAGAGTGAATCCCAGTACGTGATCCCGGATGTGTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTTGAACTCAATCCGG
ATTCTGCTCCGCGCATCCGTGTCAACGAGGCCTATGCGGCCATGGCGCGCAATGCCCGCAGCAGCACGGATACCCAGTTC
ATCCGTTCTCATTTGCAGGAGGCCAAGTGGTTCATCAAGAGCCTGGAGAGTCGTAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTTTTCGAGTACGGCGAAGAGGCCATGAAACCCATGGTACTCAACGACATTGCGG
AAGCGGTGGAGATGCATGAGTCGACCATTTCACGGGTCACGACCCAGAAGTACATGCATACGCCCCGCGGCATTTTTGAA
CTGAAGTTTTTTTTCTCCAGTCACGTCAATACGGAGGGAGGAGGAGAGTGCTCATCGACCGCTATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCGGAAAACCCCGCCAAGCCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0TRY9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.472

100

0.482