Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   LGL65_RS18795 Genome accession   NZ_CP092110
Coordinates   3640680..3641117 (+) Length   145 a.a.
NCBI ID   WP_007612572.1    Uniprot ID   -
Organism   Bacillus velezensis strain LOH112     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3635680..3646117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LGL65_RS18770 - 3635693..3636994 (-) 1302 WP_025649848.1 hemolysin family protein -
  LGL65_RS18775 - 3637140..3638090 (+) 951 WP_014418379.1 magnesium transporter CorA family protein -
  LGL65_RS18780 comGA 3638283..3639353 (+) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  LGL65_RS18785 comGB 3639340..3640377 (+) 1038 WP_014418377.1 competence type IV pilus assembly protein ComGB Machinery gene
  LGL65_RS18790 comGC 3640382..3640690 (+) 309 WP_014418376.1 competence type IV pilus major pilin ComGC Machinery gene
  LGL65_RS18795 comGD 3640680..3641117 (+) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  LGL65_RS18800 comGE 3641101..3641415 (+) 315 WP_017418140.1 competence type IV pilus minor pilin ComGE Machinery gene
  LGL65_RS18805 comGF 3641429..3641824 (+) 396 WP_025649850.1 competence type IV pilus minor pilin ComGF -
  LGL65_RS18810 comGG 3641825..3642202 (+) 378 WP_025649851.1 competence type IV pilus minor pilin ComGG Machinery gene
  LGL65_RS18815 - 3642259..3642438 (+) 180 WP_003153093.1 YqzE family protein -
  LGL65_RS18820 - 3642479..3642808 (-) 330 WP_012117979.1 DUF3889 domain-containing protein -
  LGL65_RS18825 tapA 3643067..3643738 (+) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  LGL65_RS18830 - 3643710..3644294 (+) 585 WP_012117977.1 signal peptidase I -
  LGL65_RS18835 - 3644359..3645144 (+) 786 WP_017418136.1 TasA family protein -
  LGL65_RS18840 sinR 3645192..3645527 (-) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  LGL65_RS18845 sinI 3645561..3645734 (-) 174 WP_014418369.1 anti-repressor SinI family protein Regulator

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16254.76 Da        Isoelectric Point: 10.1850

>NTDB_id=655993 LGL65_RS18795 WP_007612572.1 3640680..3641117(+) (comGD) [Bacillus velezensis strain LOH112]
MNNNRRTENGFTLLESLIVLSLASVLLTVLFTTVPPVCTHLAVRQKTEQLQKDIQLAQETAIAEHKRTKITFLPKEHKYK
LQSGGRIVERSFDSLHITLVTLPESLEFNEKGHPNSGGKIQLKSAGFTYEITVYLGSGNVHAKRK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=655993 LGL65_RS18795 WP_007612572.1 3640680..3641117(+) (comGD) [Bacillus velezensis strain LOH112]
TTGAACAATAACAGGCGGACAGAAAATGGGTTTACCCTTCTTGAAAGCCTGATTGTGTTAAGCCTGGCGTCCGTCCTGCT
GACGGTTTTGTTCACGACGGTTCCGCCGGTCTGCACCCACCTTGCCGTGCGGCAAAAAACAGAACAGCTCCAAAAAGATA
TTCAGCTTGCTCAGGAAACGGCTATCGCAGAACATAAAAGAACAAAAATCACTTTTCTTCCAAAAGAGCATAAATACAAA
CTGCAGTCAGGCGGAAGGATTGTCGAGCGTTCTTTTGATTCGCTTCACATTACGCTTGTGACTTTACCGGAGAGCCTTGA
ATTTAACGAAAAAGGCCATCCGAATTCGGGAGGAAAGATACAATTGAAGAGCGCCGGGTTCACCTATGAGATCACAGTTT
ACTTAGGGAGCGGAAATGTCCATGCTAAACGGAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Bacillus subtilis subsp. subtilis str. 168

55.479

100

0.559