Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   MA852_RS05980 Genome accession   NZ_CP092025
Coordinates   1137634..1138584 (+) Length   316 a.a.
NCBI ID   WP_002825333.1    Uniprot ID   A0A2A4L5T6
Organism   Campylobacter coli strain 14158-6     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1119744..1143389 1137634..1138584 within 0


Gene organization within MGE regions


Location: 1119744..1143389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MA852_RS05890 (MA852_05890) - 1119744..1120673 (-) 930 WP_238504951.1 glycosyltransferase family 2 protein -
  MA852_RS05895 (MA852_05895) pglH 1120666..1121745 (-) 1080 WP_134792332.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  MA852_RS05900 (MA852_05900) pglK 1121742..1123436 (-) 1695 WP_025399071.1 ABC-type lipopolysaccharide transporter PglK -
  MA852_RS05905 (MA852_05905) galE 1123430..1124416 (-) 987 WP_025399072.1 UDP-glucose 4-epimerase GalE -
  MA852_RS05910 (MA852_05910) - 1124474..1125265 (-) 792 WP_025399073.1 3'-5' exonuclease -
  MA852_RS05915 (MA852_05915) waaC 1125330..1126358 (+) 1029 WP_025399074.1 lipopolysaccharide heptosyltransferase I -
  MA852_RS05920 (MA852_05920) htrB 1126348..1127235 (+) 888 WP_238504957.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  MA852_RS05925 (MA852_05925) - 1127232..1127999 (+) 768 Protein_1150 glycosyltransferase family 2 protein -
  MA852_RS05930 (MA852_05930) - 1127992..1129053 (+) 1062 WP_238504960.1 glycosyltransferase family 4 protein -
  MA852_RS05935 (MA852_05935) - 1129050..1129913 (-) 864 WP_220575213.1 glycosyltransferase family 2 protein -
  MA852_RS09585 - 1130880..1131114 (-) 235 Protein_1153 glycosyltransferase family 2 protein -
  MA852_RS05945 (MA852_05945) - 1131192..1132163 (-) 972 WP_238504962.1 glycosyltransferase family 2 protein -
  MA852_RS05950 (MA852_05950) - 1132252..1133131 (+) 880 Protein_1155 alpha-2,3-sialyltransferase -
  MA852_RS05955 (MA852_05955) neuB 1133132..1134172 (+) 1041 WP_057044594.1 N-acetylneuraminate synthase -
  MA852_RS05960 (MA852_05960) neuC 1134169..1135287 (+) 1119 WP_002878821.1 UDP-N-acetylglucosamine 2-epimerase -
  MA852_RS05965 (MA852_05965) - 1135284..1135949 (+) 666 WP_057044595.1 cytidylyltransferase domain-containing protein -
  MA852_RS05970 (MA852_05970) - 1136073..1136771 (+) 699 WP_228761796.1 CatB-related O-acetyltransferase -
  MA852_RS05975 (MA852_05975) - 1136763..1137575 (-) 813 WP_057044596.1 glycosyltransferase family 2 protein -
  MA852_RS05980 (MA852_05980) waaF 1137634..1138584 (+) 951 WP_002825333.1 lipopolysaccharide heptosyltransferase II Regulator
  MA852_RS05985 (MA852_05985) - 1138570..1139334 (-) 765 WP_057044597.1 glycosyltransferase family 25 protein -
  MA852_RS05990 (MA852_05990) gmhA 1139334..1139894 (-) 561 WP_057044598.1 D-sedoheptulose 7-phosphate isomerase -
  MA852_RS05995 (MA852_05995) rfaE1 1139891..1141276 (-) 1386 WP_057044599.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  MA852_RS06000 (MA852_06000) rfaD 1141269..1142222 (-) 954 WP_002867398.1 ADP-glyceromanno-heptose 6-epimerase -
  MA852_RS06005 (MA852_06005) - 1142223..1142783 (-) 561 WP_057044600.1 D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase -
  MA852_RS06010 (MA852_06010) - 1142860..1143159 (+) 300 WP_002777238.1 cytochrome c -
  MA852_RS06015 (MA852_06015) - 1143189..1143389 (-) 201 WP_002777240.1 cbb3-type cytochrome oxidase assembly protein -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 36243.74 Da        Isoelectric Point: 10.2421

>NTDB_id=655178 MA852_RS05980 WP_002825333.1 1137634..1138584(+) (waaF) [Campylobacter coli strain 14158-6]
MKIFIHLPTWLGDAVMASPALYAIKEHFKNAQFILYGSLVSTALFKEFSNSKIIIENKQSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLSIKAHFKDLKLPFKLKFQNPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGTGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKIKTVAIFGPTKFTQTSPWQNENATLVHLDLACMPCMQKTCPLKHHKCMKDLKPEVIVKAIQNLINI

Nucleotide


Download         Length: 951 bp        

>NTDB_id=655178 MA852_RS05980 WP_002825333.1 1137634..1138584(+) (waaF) [Campylobacter coli strain 14158-6]
ATGAAAATTTTTATACACCTTCCCACTTGGCTAGGCGATGCAGTGATGGCTTCGCCTGCTTTATACGCTATAAAAGAACA
TTTTAAAAATGCTCAGTTTATCCTTTATGGATCTTTGGTTTCCACAGCACTTTTTAAAGAATTTTCTAATTCTAAAATCA
TCATAGAAAATAAACAATCTCGCTATAAACAAGCCCTATCTTTACGCAAAGAACTTGGCAAAATCGATTTTAGCTTTGCT
TTTAGGTCTGCGTTTTCTTCTAAGATTATCTTACATATTCTTAAAACAAAGCAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTAGTATAAAAGCTCATTTTAAAGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTGTCTTAAAAAATGGTAAAAAAATTCTAGGACTCAACCCTGGTGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAGATTTT
AATCTTTGGCACAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGCCCTATGCACATAAGTGCGGTCTATAAGATAAAAACCGTGGCTATTTTTGGTCCTACGAAATTTACTCAAACCTCACC
TTGGCAAAACGAAAATGCAACATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAGCCTGAAGTGATTGTAAAAGCGATTCAAAATTTAATTAATATCTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(96-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A4L5T6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

92.675

99.367

0.921