Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   MA544_RS06435 Genome accession   NZ_CP092017
Coordinates   1230316..1231266 (+) Length   316 a.a.
NCBI ID   WP_057035865.1    Uniprot ID   -
Organism   Campylobacter jejuni subsp. jejuni strain 15065A     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1218318..1244252 1230316..1231266 within 0


Gene organization within MGE regions


Location: 1218318..1244252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MA544_RS06380 (MA544_06380) pglI 1218318..1219247 (-) 930 WP_002859236.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  MA544_RS06385 (MA544_06385) pglH 1219240..1220319 (-) 1080 WP_079744322.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  MA544_RS06390 (MA544_06390) pglK 1220316..1222010 (-) 1695 WP_057035978.1 ABC-type lipopolysaccharide transporter PglK -
  MA544_RS06395 (MA544_06395) galE 1222004..1222990 (-) 987 WP_002872230.1 UDP-glucose 4-epimerase GalE -
  MA544_RS06400 (MA544_06400) - 1223048..1223842 (-) 795 WP_002872231.1 3'-5' exonuclease -
  MA544_RS06405 (MA544_06405) waaC 1223908..1224936 (+) 1029 WP_057035979.1 lipopolysaccharide heptosyltransferase I -
  MA544_RS06410 (MA544_06410) htrB 1224926..1225813 (+) 888 WP_057035980.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  MA544_RS06415 (MA544_06415) - 1225810..1227360 (+) 1551 WP_238523066.1 glycosyltransferase -
  MA544_RS06420 (MA544_06420) - 1227329..1228216 (-) 888 WP_238523067.1 glycosyltransferase family 2 protein -
  MA544_RS06425 (MA544_06425) - 1228242..1229387 (-) 1146 WP_057035867.1 glycosyltransferase family 8 protein -
  MA544_RS06430 (MA544_06430) waaV 1229436..1230257 (-) 822 WP_057035866.1 glycosyltransferase family 2 protein -
  MA544_RS06435 (MA544_06435) waaF 1230316..1231266 (+) 951 WP_057035865.1 lipopolysaccharide heptosyltransferase II Regulator
  MA544_RS06440 (MA544_06440) - 1231252..1232016 (-) 765 WP_002872239.1 glycosyltransferase family 25 protein -
  MA544_RS06445 (MA544_06445) gmhA 1232016..1232576 (-) 561 WP_057035864.1 D-sedoheptulose 7-phosphate isomerase -
  MA544_RS06450 (MA544_06450) rfaE1 1232573..1233958 (-) 1386 WP_057035863.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  MA544_RS06455 (MA544_06455) rfaD 1233951..1234904 (-) 954 WP_002867398.1 ADP-glyceromanno-heptose 6-epimerase -
  MA544_RS06460 (MA544_06460) gmhB 1234905..1235466 (-) 562 Protein_1268 HAD family hydrolase -
  MA544_RS06465 (MA544_06465) - 1235547..1235849 (+) 303 WP_002852762.1 cytochrome c -
  MA544_RS06470 (MA544_06470) ccoS 1235872..1236078 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  MA544_RS06475 (MA544_06475) - 1236075..1238432 (-) 2358 WP_079254352.1 heavy metal translocating P-type ATPase -
  MA544_RS06480 (MA544_06480) rho 1238541..1239839 (+) 1299 WP_002852852.1 transcription termination factor Rho -
  MA544_RS06485 (MA544_06485) dnaX 1239843..1241372 (+) 1530 WP_002867395.1 DNA polymerase III subunit gamma/tau -
  MA544_RS06490 (MA544_06490) - 1241369..1241887 (-) 519 Protein_1274 DUF2165 family protein -
  MA544_RS06495 (MA544_06495) copA 1241959..1244058 (-) 2100 WP_057035862.1 copper-translocating P-type ATPase CopA -
  MA544_RS06500 (MA544_06500) - 1244058..1244252 (-) 195 WP_002852760.1 cation transporter -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 36395.01 Da        Isoelectric Point: 10.3316

>NTDB_id=655079 MA544_RS06435 WP_057035865.1 1230316..1231266(+) (waaF) [Campylobacter jejuni subsp. jejuni strain 15065A]
MKIFIHLPTWLGDAVMASPALYVIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLHIKAHLKDLKLPFKLKFQNPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNQNARWVHLDLACMPCMQKTCPLKHHKCMKDLKPEVILKAIQNLINI

Nucleotide


Download         Length: 951 bp        

>NTDB_id=655079 MA544_RS06435 WP_057035865.1 1230316..1231266(+) (waaF) [Campylobacter jejuni subsp. jejuni strain 15065A]
ATGAAAATTTTTATACACCTTCCCACTTGGCTAGGCGATGCGGTGATGGCTTCACCTGCTTTATACGTTATAAAAGAACA
TTTTAAAAATGCCCAATTTATCCTTTATGGATCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCCCGCTACAAACAAGCCCTATCTTTACGCAAAGAACTTGGTAAGATCGATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTACATATTCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTCACATAAAAGCTCACCTTAAAGACTTAA
AACTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTGTCTTAAAAAATGGCAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCACGAGATTTT
AATCTTTGGCGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACTACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGACCTATGCACATAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATCAAAATGCAAGATGGGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAACCTGAAGTGATTTTAAAGGCAATTCAAAATTTAATTAATATCTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(96-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

92.357

99.367

0.918