Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LXB09_RS03615 Genome accession   NZ_CP091981
Coordinates   768352..769731 (-) Length   459 a.a.
NCBI ID   WP_135014592.1    Uniprot ID   -
Organism   Lactobacillus johnsonii strain MR1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 763352..774731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LXB09_RS03595 (LXB09_03595) rlmB 764091..764846 (-) 756 WP_135014589.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LXB09_RS03600 (LXB09_03600) - 764833..765276 (-) 444 WP_004895772.1 Mini-ribonuclease 3 -
  LXB09_RS03605 (LXB09_03605) cysS 765269..766681 (-) 1413 WP_162222133.1 cysteine--tRNA ligase -
  LXB09_RS03610 (LXB09_03610) gltX 766771..768270 (-) 1500 WP_135014591.1 glutamate--tRNA ligase -
  LXB09_RS03615 (LXB09_03615) radA 768352..769731 (-) 1380 WP_135014592.1 DNA repair protein RadA Machinery gene
  LXB09_RS03620 (LXB09_03620) - 769731..770282 (-) 552 WP_004896724.1 dUTP diphosphatase -
  LXB09_RS03625 (LXB09_03625) - 770399..771433 (-) 1035 WP_135014593.1 LCP family protein -
  LXB09_RS03630 (LXB09_03630) pepC 771631..772980 (+) 1350 WP_135014594.1 aminopeptidase C -
  LXB09_RS03635 (LXB09_03635) - 773262..774275 (+) 1014 WP_238461405.1 sugar transferase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50119.68 Da        Isoelectric Point: 7.6369

>NTDB_id=654861 LXB09_RS03615 WP_135014592.1 768352..769731(-) (radA) [Lactobacillus johnsonii strain MR1]
MAKVKTRYKCRNCGYISASYLGRCPNCGAWNQFEEETQEIKKVSTKATASRLMTKIGNNDPVKLTEVKAEKEKRIVTPFE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQITGALAKEHSVLYVSGEESASQIKMRADRLGVSNSGILLYPETNMQNI
RDQIDEIKPDFLVIDSIQTMNEPSLDSMVGSASQVREVTSELMKIAKNEQITTFVIGHVTKEGAIAGPKIMEHMVDTVLY
FEGDGHHSYRILRSVKNRFGAANEIGMFEMKNEGLAEVSNPSAIFLDERLPNSTGSSVVVSLEGTRPLLADIQALVTPTA
FGYAKRTTSGLDFNRVALLLAVLEKRGNLMLQNQDAFLTATGGIKLNEPAIDLAICMAVASSYKNKEISSTDCFVGEVGL
TGEIRRVNQIEARVREAAKVGFKRIFIPKNNLSQELKNNSEIEVIGVASLPQALKLVFN

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=654861 LXB09_RS03615 WP_135014592.1 768352..769731(-) (radA) [Lactobacillus johnsonii strain MR1]
ATGGCAAAAGTTAAAACTCGTTATAAGTGTCGTAATTGTGGATATATTTCTGCTTCCTATCTTGGAAGATGTCCCAATTG
TGGTGCTTGGAATCAATTTGAAGAAGAGACACAGGAAATTAAAAAAGTATCAACTAAGGCAACTGCAAGTCGGTTGATGA
CTAAAATTGGAAATAATGATCCAGTAAAGTTAACTGAAGTAAAAGCTGAGAAAGAAAAAAGAATTGTGACTCCTTTTGAA
GAATTAAATCGAGTTTTAGGAGGAGGGATTGTTCCAGGATCTTTAGTTTTAATTGGAGGAGATCCAGGAATTGGAAAATC
AACCTTAATGCTTCAAATTACTGGTGCTTTAGCTAAAGAACATAGCGTCTTATATGTTTCAGGAGAAGAGTCCGCAAGTC
AAATAAAGATGCGGGCAGATCGATTAGGAGTAAGCAATAGCGGAATTTTGCTTTATCCAGAAACAAATATGCAGAATATT
CGTGACCAGATTGATGAAATTAAGCCAGATTTTTTGGTAATTGATTCTATTCAAACAATGAATGAACCTTCACTTGATTC
AATGGTTGGATCTGCTTCTCAAGTACGTGAAGTAACAAGTGAACTAATGAAAATTGCTAAAAATGAACAAATAACTACTT
TTGTTATTGGCCATGTAACTAAAGAAGGAGCAATTGCTGGTCCTAAGATTATGGAACATATGGTTGATACCGTCCTTTAC
TTTGAAGGGGATGGACACCACTCATATCGAATTTTAAGATCAGTCAAGAATCGTTTTGGCGCAGCTAATGAAATTGGAAT
GTTTGAGATGAAAAATGAAGGGTTAGCAGAAGTAAGTAACCCTTCGGCTATTTTCTTAGACGAACGCTTACCTAATTCGA
CTGGTTCCTCAGTTGTTGTTTCACTTGAAGGAACGCGGCCTCTTCTTGCGGATATTCAAGCTTTAGTAACTCCAACTGCA
TTTGGATATGCGAAAAGAACAACTTCAGGATTAGATTTTAATCGAGTAGCACTTTTATTGGCAGTTTTAGAAAAAAGAGG
CAACTTAATGCTTCAAAATCAGGATGCTTTTCTAACTGCAACTGGTGGGATTAAATTAAATGAACCAGCGATTGATCTTG
CAATTTGCATGGCGGTTGCTTCAAGTTATAAAAATAAAGAAATTTCTTCTACTGATTGCTTTGTAGGTGAAGTTGGATTA
ACTGGAGAAATTAGAAGAGTTAATCAAATTGAAGCTAGAGTCAGAGAGGCCGCAAAAGTAGGCTTTAAGAGGATTTTTAT
TCCTAAAAATAACTTAAGCCAGGAACTTAAAAATAACTCTGAGATCGAAGTAATTGGAGTAGCAAGTTTACCACAAGCTT
TAAAACTTGTTTTTAACTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-223)

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

60.262

99.782

0.601
  radA Streptococcus pneumoniae Rx1

60.262

99.782

0.601
  radA Streptococcus pneumoniae D39

60.262

99.782

0.601
  radA Streptococcus pneumoniae R6

60.262

99.782

0.601
  radA Streptococcus pneumoniae TIGR4

60.262

99.782

0.601
  radA Streptococcus mitis SK321

60.262

99.782

0.601
  radA Bacillus subtilis subsp. subtilis str. 168

55.921

99.346

0.556