Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   L8M16_RS11845 Genome accession   NZ_CP091977
Coordinates   2462075..2463442 (-) Length   455 a.a.
NCBI ID   WP_011239482.1    Uniprot ID   Q5NYN5
Organism   Aromatoleum aromaticum strain pCyN1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2457075..2468442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L8M16_RS11835 - 2460041..2460436 (-) 396 WP_011239480.1 response regulator -
  L8M16_RS11840 - 2460439..2461914 (-) 1476 WP_011239481.1 PAS domain-containing sensor histidine kinase -
  L8M16_RS11845 radA 2462075..2463442 (-) 1368 WP_011239482.1 DNA repair protein RadA Machinery gene
  L8M16_RS11850 - 2463442..2464362 (-) 921 WP_011239483.1 LysR family transcriptional regulator -
  L8M16_RS11855 lplT 2464540..2465790 (+) 1251 WP_011239484.1 lysophospholipid transporter LplT -
  L8M16_RS11860 - 2465816..2466637 (-) 822 WP_011239485.1 uracil-DNA glycosylase -
  L8M16_RS11865 rimI 2466634..2467089 (-) 456 WP_011239486.1 ribosomal protein S18-alanine N-acetyltransferase -
  L8M16_RS11870 tsaB 2467086..2467778 (-) 693 WP_011239487.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -
  L8M16_RS11875 - 2467892..2468155 (-) 264 WP_168953339.1 hypothetical protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48688.02 Da        Isoelectric Point: 6.8885

>NTDB_id=654824 L8M16_RS11845 WP_011239482.1 2462075..2463442(-) (radA) [Aromatoleum aromaticum strain pCyN1]
MAKAKTVFSCTECGAQAPRWQGQCPQCKAWNTLVEAVAEAGGGSRFAALAGSTAQLQTLAELEPREEPRTPTGIDEFDRV
LGGGLVAGGVVLIGGDPGIGKSTLLLQALSVLAGRHKAIYVSGEESGEQVALRAQRLQLDPAGLQLLAEINLERILAMLR
DERPRIVVIDSIQTVYSEVLQSAPGSVAQVRECAAQLTRYAKQSGTCMIMVGHVTKDGTLAGPRVLEHMVDTVLYFEGDT
HSSFRLVRAFKNRFGAVNELGVFAMTERGLRGVSNPSALFLSQHSQQVSGSCVLVTQEGTRPLLVEIQALVDGTHSPNPR
RLSVGLEQNRLAMLLAVLHRHAAVVCFDQDVFVNAVGGVKIAEPAADLAVLLAIVSSLRNRPLPRELVVFGEVGLAGEIR
PAPRGQERLREAAKLGFTTAIIPKANAPRQALGDMQVIAVDRIEEAIDRARELEG

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=654824 L8M16_RS11845 WP_011239482.1 2462075..2463442(-) (radA) [Aromatoleum aromaticum strain pCyN1]
ATGGCGAAAGCGAAGACGGTTTTCAGCTGCACCGAATGCGGCGCGCAGGCGCCGCGCTGGCAGGGGCAATGCCCGCAGTG
CAAGGCGTGGAACACGCTCGTCGAAGCGGTGGCCGAAGCCGGTGGTGGTAGCCGGTTCGCGGCGCTGGCGGGCAGCACTG
CCCAGCTGCAGACGCTGGCCGAACTCGAGCCGCGCGAAGAGCCCCGCACCCCCACCGGGATCGACGAGTTCGACCGGGTG
CTGGGTGGCGGGCTGGTCGCGGGCGGCGTCGTGCTGATCGGCGGCGACCCGGGCATCGGCAAATCGACGCTGCTGCTGCA
GGCACTGTCGGTGCTGGCCGGCCGGCACAAGGCGATCTACGTGAGCGGCGAGGAGTCGGGCGAACAGGTGGCGCTGCGGG
CGCAGCGCCTGCAGCTCGATCCCGCCGGGCTGCAGCTGCTCGCGGAGATCAACCTGGAACGGATCCTGGCGATGCTCCGC
GACGAACGCCCGCGCATCGTGGTGATCGACTCGATCCAGACAGTGTATTCCGAAGTGCTCCAGTCGGCGCCCGGCTCGGT
CGCGCAGGTGCGCGAATGTGCCGCGCAGCTGACCCGCTACGCGAAGCAGAGCGGTACCTGCATGATCATGGTCGGCCATG
TGACGAAGGACGGCACGCTGGCCGGTCCGCGCGTGCTCGAGCACATGGTGGACACCGTGCTGTACTTCGAAGGCGACACC
CATTCGAGCTTCCGGCTGGTGCGCGCGTTCAAGAACCGCTTCGGCGCGGTCAATGAACTCGGCGTGTTCGCGATGACCGA
GCGCGGGTTGCGCGGCGTCAGCAATCCGTCGGCGCTTTTCCTGTCGCAGCACAGCCAGCAGGTGTCGGGCAGCTGCGTGC
TGGTCACGCAGGAAGGCACGCGTCCGCTGCTCGTCGAGATCCAGGCGCTCGTCGATGGGACGCACAGCCCGAATCCGCGA
CGGCTGTCGGTCGGACTCGAACAGAACCGGCTGGCTATGCTCCTGGCCGTGCTGCACCGGCACGCCGCGGTCGTGTGCTT
CGATCAGGACGTGTTCGTCAATGCCGTCGGGGGGGTGAAAATCGCCGAGCCTGCCGCGGATCTCGCAGTGCTGCTGGCGA
TCGTGTCGTCGCTGCGCAACCGCCCGCTGCCACGCGAGCTGGTGGTGTTCGGCGAAGTCGGGCTGGCGGGCGAAATCCGC
CCGGCGCCGCGCGGCCAGGAGCGGCTCCGGGAAGCGGCGAAGCTCGGCTTCACCACCGCGATCATCCCCAAGGCCAACGC
TCCGCGCCAGGCGCTCGGCGACATGCAGGTGATAGCGGTCGATCGCATCGAGGAGGCGATCGACCGCGCTCGCGAGCTCG
AAGGCTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(80-218)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(357-450)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5NYN5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.904

100

0.49
  radA Streptococcus mitis SK321

47.137

99.78

0.47
  radA Streptococcus pneumoniae D39

47.45

99.121

0.47
  radA Streptococcus pneumoniae TIGR4

47.45

99.121

0.47
  radA Streptococcus pneumoniae R6

47.45

99.121

0.47
  radA Streptococcus pneumoniae Rx1

47.45

99.121

0.47
  radA Streptococcus mitis NCTC 12261

46.813

100

0.468