Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   L8M16_RS01465 Genome accession   NZ_CP091977
Coordinates   319370..320803 (-) Length   477 a.a.
NCBI ID   WP_168953800.1    Uniprot ID   -
Organism   Aromatoleum aromaticum strain pCyN1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 314370..325803
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L8M16_RS01440 - 315407..315904 (+) 498 WP_011237731.1 5-formyltetrahydrofolate cyclo-ligase -
  L8M16_RS01445 ilvA 315914..317434 (-) 1521 WP_168953798.1 threonine ammonia-lyase, biosynthetic -
  L8M16_RS01450 hprK 317506..318447 (-) 942 WP_168953799.1 HPr(Ser) kinase/phosphatase -
  L8M16_RS01455 ptsN 318416..318901 (-) 486 WP_011237734.1 PTS IIA-like nitrogen regulatory protein PtsN -
  L8M16_RS01460 hpf 319032..319355 (-) 324 WP_011237735.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  L8M16_RS01465 rpoN/rpoN1 319370..320803 (-) 1434 WP_168953800.1 RNA polymerase factor sigma-54 Machinery gene
  L8M16_RS01470 lptB 320824..321546 (-) 723 WP_168953801.1 LPS export ABC transporter ATP-binding protein -
  L8M16_RS01475 recX 321533..321982 (-) 450 WP_168953802.1 recombination regulator RecX -
  L8M16_RS01480 recA 321993..323033 (-) 1041 WP_011237739.1 recombinase RecA Machinery gene
  L8M16_RS01485 - 323096..323596 (-) 501 WP_168953803.1 CinA family protein -
  L8M16_RS01490 - 323598..324077 (-) 480 WP_041647110.1 phosphatidylglycerophosphatase A -
  L8M16_RS01495 thiL 324135..325094 (-) 960 WP_168953804.1 thiamine-phosphate kinase -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53295.28 Da        Isoelectric Point: 5.0057

>NTDB_id=654811 L8M16_RS01465 WP_168953800.1 319370..320803(-) (rpoN/rpoN1) [Aromatoleum aromaticum strain pCyN1]
MKPTLQLKLSQHLTLTPQLQQSIKLLQLSTLELNQELERFLLENPMLEREDGDGSDFVPPPATAAATGGENENPPAASEQ
HPDAGPANLDEAGEWSSAGSGSSNRDDEDDSDYQEFQAAETSLRDHLDQQVSLSPLSDRDRALVRFIIEALDDDGYLNQS
LEELLPLLPPELEYDLDDLSIALHHVQNLDPAGVAARTPQECLSLQLRAMRPTPARELALKIVDHHLELLAERNFVRLKR
LLGCDDDALRCAHLLICSLDPHPGSQHSVQETRYIMPDVVVRKQRGRWVVTLNPEAMPKLRINSLYASILQQNRGSGGAL
TSQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDYGEVAMRPLTLREIADQLELHESTVSRVTTQKFMATPRGVFEL
KYFFGSHVATDTGGAASSTAIRALIRQLVEAEDRKKPLSDAKIAELLGQQGIVVARRTIAKYRESLNIPPVSLRKTI

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=654811 L8M16_RS01465 WP_168953800.1 319370..320803(-) (rpoN/rpoN1) [Aromatoleum aromaticum strain pCyN1]
ATGAAGCCGACCCTCCAGCTCAAACTCTCCCAGCACCTCACGCTGACACCGCAACTGCAGCAGTCGATCAAGCTGTTGCA
GTTATCGACGCTCGAACTGAACCAGGAGCTCGAACGCTTCCTGCTCGAGAACCCGATGCTCGAACGCGAGGACGGCGACG
GCAGCGATTTTGTCCCCCCGCCCGCCACGGCCGCAGCTACAGGCGGCGAAAACGAAAATCCTCCCGCGGCGAGCGAACAG
CACCCCGACGCGGGACCGGCGAACCTCGACGAAGCCGGTGAGTGGTCGAGCGCGGGAAGCGGCTCGTCGAACCGCGACGA
CGAGGACGACAGCGACTACCAGGAGTTCCAGGCGGCGGAAACCAGCCTGCGCGATCACCTCGACCAGCAGGTCAGCCTGT
CGCCGCTGTCCGATCGCGACCGCGCGCTGGTGCGCTTCATCATCGAGGCGCTCGACGACGACGGCTATCTGAACCAGTCG
CTCGAAGAGCTGCTGCCGCTGTTGCCGCCGGAACTCGAATACGACCTCGACGACCTGTCGATCGCACTACACCATGTGCA
GAACCTCGACCCCGCAGGCGTCGCGGCGCGCACGCCGCAGGAATGCCTCAGCCTGCAGCTGCGCGCGATGCGCCCGACTC
CAGCGCGCGAGCTCGCGCTGAAGATCGTCGACCATCACCTCGAGCTTCTTGCCGAACGCAACTTCGTCCGCCTCAAGCGC
CTGCTCGGTTGCGACGACGATGCGCTGCGCTGCGCGCACCTGCTGATCTGCAGCCTCGACCCGCACCCGGGCTCCCAGCA
TTCAGTGCAGGAAACGCGCTACATCATGCCCGACGTCGTCGTGCGCAAGCAGCGCGGGCGCTGGGTCGTCACGCTGAATC
CCGAAGCGATGCCGAAACTGCGCATCAACTCGCTGTACGCCAGCATCCTGCAGCAGAATCGCGGCTCCGGCGGCGCCTTG
ACGAGTCAGTTGCAGGAAGCGCGCTGGCTGATCAAGAACGTGCAGCAGCGTTTCGACACGATCCTGCGCGTCTCCCAGGC
GATCGTTGACCAGCAGCGGCAGTTCTTCGATTATGGCGAGGTGGCGATGCGCCCGCTGACGCTGAGGGAAATCGCGGACC
AACTGGAACTGCACGAATCCACCGTTTCGCGGGTCACGACACAGAAATTCATGGCCACCCCGCGAGGCGTGTTCGAACTG
AAGTATTTTTTCGGCAGTCACGTCGCCACCGATACGGGTGGAGCGGCATCCTCAACGGCGATTCGCGCGCTGATCCGCCA
GTTGGTCGAAGCCGAAGACAGAAAAAAACCTCTGTCCGACGCAAAGATTGCCGAATTGTTGGGCCAGCAGGGTATCGTGG
TGGCGCGGCGCACCATTGCAAAATACCGCGAATCACTCAATATCCCGCCGGTCAGCCTGCGCAAGACGATTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(113-306)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

56.375

100

0.593