Detailed information NTG0654516

Bioinformatically predicted

Overview

• Name: pepF
• Type: Regulator
• Locus tag: L5I21_RS11600
• Genome accession: NZ_CP091899
• Coordinates: 2385192..2387003 (-)
• Length: 603 a.a.
• NCBI ID: WP_002387169.1
• Uniprot ID: A0A855UCR5
• Organism: Enterococcus faecalis strain 101-1
• Function: degradation of XIP; competence shut-off (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
ICE	2378132..2397490	2385192..2387003	within	0

Gene organization within MGE regions

Location: 2378132..2397490

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L5I21_RS11565 (L5I21_11565)	cutC	2379078..2379707 (+)	630	WP_002356490.1	copper homeostasis protein CutC	-
L5I21_RS11570 (L5I21_11570)	mgtE	2379825..2381186 (-)	1362	WP_002362548.1	magnesium transporter	-
L5I21_RS11575 (L5I21_11575)	-	2381217..2382110 (-)	894	WP_002356487.1	RluA family pseudouridine synthase	-
L5I21_RS11580 (L5I21_11580)	-	2382118..2382915 (-)	798	WP_002356486.1	NAD kinase	-
L5I21_RS11585 (L5I21_11585)	-	2382928..2383602 (-)	675	WP_002356484.1	GTP pyrophosphokinase	-
L5I21_RS11590 (L5I21_11590)	-	2383799..2384377 (+)	579	WP_002356483.1	CYTH domain-containing protein	-
L5I21_RS11595 (L5I21_11595)	-	2384441..2385100 (+)	660	WP_002368840.1	ClpXP adapter SpxH family protein	-
L5I21_RS11600 (L5I21_11600)	pepF	2385192..2387003 (-)	1812	WP_002387169.1	oligoendopeptidase F	Regulator
L5I21_RS11605 (L5I21_11605)	-	2387051..2388229 (-)	1179	WP_002387170.1	competence protein CoiA	-
L5I21_RS11610 (L5I21_11610)	-	2388319..2388981 (-)	663	WP_002356478.1	adaptor protein MecA	-
L5I21_RS11615 (L5I21_11615)	spxA	2389323..2389721 (-)	399	WP_002356477.1	transcriptional regulator SpxA	-
L5I21_RS11620 (L5I21_11620)	dfrF	2390191..2390685 (-)	495	WP_002322211.1	trimethoprim-resistant dihydrofolate reductase DfrF	-
L5I21_RS11625 (L5I21_11625)	-	2390993..2391472 (-)	480	WP_002322212.1	MGMT family protein	-
L5I21_RS11630 (L5I21_11630)	-	2391783..2392454 (-)	672	WP_002322214.1	helix-turn-helix domain-containing protein	-
L5I21_RS11635 (L5I21_11635)	-	2393048..2394058 (+)	1011	WP_009274733.1	replication initiation factor domain-containing protein	-
L5I21_RS11640 (L5I21_11640)	-	2394058..2394261 (+)	204	WP_002607397.1	excisionase	-
L5I21_RS11645 (L5I21_11645)	-	2394340..2395533 (+)	1194	WP_010706472.1	site-specific integrase	-
L5I21_RS11650 (L5I21_11650)	trpS	2395887..2396897 (+)	1011	WP_002378619.1	tryptophan--tRNA ligase	-

Sequence

Protein

• Length: 603 a.a.
• Molecular weight: 68848.16 Da
• Isoelectric Point: 4.6861

>NTDB_id=654516 L5I21_RS11600 WP_002387169.1 2385192..2387003(-) (pepF) [Enterococcus faecalis strain 101-1]
MSEIKQLPTRDEVPTPLTWDLTKIFKDDAAFDVAYNQLIEELNQAESFKGTLGDGAEAFLAALEYVLDVYRKVETLYVYS
HLKNDQDTTNTAYQALYARASSLYAQVSEAVSWFDPEVLTLSDEQIWGYFEEQPKLAVYRHYIQNILDERPHVLSMEQEA
LLAGASEIFGASSNTFSILNNADLEFPTVQNAEGETIQLSHGVYGQLMESVDPSVREAAFKGLYKVYKQFRNTLASTLGA
HVKTHNYKAKIRNYDSARAASLASNHIPESVHETLVAVVNKHLPLLHRYVKLRKKLLNVEELHMYDLYAPLLGEAPIRYS
YEEAKEKAIEALKPLGEDYLSIVKEAFSSRWIDVIENQGKRSGAYSSGAYDTAPYILMNWHDSLDQLFTLVHEMGHSVHS
YYTRNNQPYVYGDYSIFLAEIASTTNENILTEYLLQTETDPKVRAYVLNHYLDGFKGTIFRQTQFAEFEHFIHTEDAKGT
PLTSEYLSEYYGELNAKYYGPEVVRDEEISYEWARIPHFYYNYYVYQYATGFSAASALSKHILAGEEGALENYLNYLKAG
SSDFPIEVMKKAGVDMTQAAYIEDAMKVFEERLTELEALVEKL

Nucleotide

• Length: 1812 bp

>NTDB_id=654516 L5I21_RS11600 WP_002387169.1 2385192..2387003(-) (pepF) [Enterococcus faecalis strain 101-1]
ATGAGCGAAATTAAACAATTACCAACACGTGATGAAGTTCCAACTCCATTGACTTGGGATTTAACCAAAATTTTTAAAGA
TGATGCTGCTTTTGATGTTGCGTATAATCAGTTGATAGAAGAGCTAAATCAAGCAGAATCATTTAAAGGTACCTTAGGGG
ATGGTGCAGAAGCTTTTCTAGCTGCGTTAGAATACGTTTTAGATGTCTATCGAAAAGTGGAAACATTATATGTCTATTCT
CATTTAAAAAATGATCAAGACACTACAAATACAGCTTACCAAGCTTTATATGCCAGAGCTAGTTCTTTATATGCACAAGT
TAGTGAAGCTGTTTCTTGGTTTGACCCAGAGGTTTTAACATTAAGTGATGAACAAATCTGGGGATATTTTGAAGAGCAGC
CGAAATTAGCTGTTTATCGTCACTATATTCAAAATATTTTAGATGAACGTCCCCATGTTTTATCGATGGAACAAGAAGCT
TTACTGGCGGGGGCTAGTGAAATTTTTGGTGCCTCAAGTAATACATTTTCAATTTTAAATAATGCAGACTTAGAATTTCC
AACTGTGCAAAATGCTGAAGGCGAAACGATTCAACTTTCTCATGGCGTTTATGGTCAGTTAATGGAAAGTGTCGATCCAT
CTGTTCGTGAAGCAGCATTTAAAGGTTTGTACAAAGTATACAAACAATTTAGAAATACATTAGCCTCAACTTTAGGTGCA
CATGTTAAAACACATAATTATAAAGCAAAAATTAGAAATTATGATTCTGCTCGAGCAGCCTCTTTAGCAAGTAATCATAT
CCCTGAAAGTGTTCACGAAACATTAGTAGCTGTAGTAAATAAACATTTACCTTTGCTACATCGTTATGTAAAATTAAGAA
AAAAATTATTAAACGTAGAAGAATTACACATGTACGACTTGTATGCGCCTTTGCTAGGTGAAGCACCAATTCGTTACAGC
TATGAAGAAGCAAAAGAAAAAGCAATTGAGGCTTTAAAACCACTAGGTGAAGACTATTTATCTATTGTTAAAGAAGCTTT
TTCAAGTCGCTGGATTGATGTGATTGAAAATCAAGGAAAACGAAGCGGCGCATATTCTTCAGGAGCTTATGATACAGCCC
CATACATTTTAATGAATTGGCATGATAGTTTGGATCAACTATTTACATTAGTCCATGAGATGGGCCATAGTGTTCATAGT
TACTATACAAGAAATAATCAGCCGTATGTTTATGGCGACTATTCAATTTTCTTAGCTGAGATTGCTTCAACCACAAATGA
AAATATTTTAACAGAATATTTATTACAAACAGAAACAGATCCTAAAGTACGTGCGTATGTCTTAAATCACTATTTAGACG
GCTTTAAGGGAACCATTTTCCGTCAAACGCAATTTGCGGAATTTGAACATTTTATTCACACGGAAGATGCTAAAGGCACG
CCATTAACAAGTGAATATTTGAGTGAGTATTATGGCGAGCTCAATGCTAAATATTATGGACCAGAAGTAGTTAGAGACGA
AGAAATCAGTTACGAATGGGCGAGAATTCCACATTTTTATTACAATTACTATGTTTATCAATATGCAACTGGCTTCTCAG
CCGCTTCGGCATTGTCTAAACATATTTTAGCTGGAGAAGAGGGAGCTTTAGAGAATTATCTTAACTACTTGAAAGCAGGA
AGTAGTGACTTCCCAATTGAAGTGATGAAAAAAGCGGGCGTGGATATGACACAAGCCGCGTATATCGAAGATGCAATGAA
AGTTTTTGAAGAACGTTTAACGGAATTAGAAGCTTTGGTTGAAAAATTATAG

Domains

Predicted by InterproScan.

• Peptidase family M3 (Peptidase_M3) (207-587)

• Oligopeptidase F (Peptidase_M3_N) (117-186)

3D structure

• AlphaFold DB: A0A855UCR5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pepF	Streptococcus salivarius strain HSISS4	54.806	98.342	0.539