Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   L3V59_RS16060 Genome accession   NZ_CP091646
Coordinates   3389868..3391373 (+) Length   501 a.a.
NCBI ID   WP_122946298.1    Uniprot ID   -
Organism   Burkholderia aenigmatica strain CMCC(B)23010     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3384868..3396373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3V59_RS16035 (L3V59_16035) kdsD 3386114..3387097 (+) 984 WP_122946295.1 arabinose 5-phosphate isomerase KdsD -
  L3V59_RS16040 (L3V59_16040) - 3387097..3387633 (+) 537 WP_043187330.1 HAD family hydrolase -
  L3V59_RS16045 (L3V59_16045) lptC 3387637..3388251 (+) 615 WP_174955661.1 LPS export ABC transporter periplasmic protein LptC -
  L3V59_RS16050 (L3V59_16050) lptA 3388272..3388937 (+) 666 WP_122946297.1 lipopolysaccharide transport periplasmic protein LptA -
  L3V59_RS16055 (L3V59_16055) lptB 3388934..3389710 (+) 777 WP_034189275.1 LPS export ABC transporter ATP-binding protein -
  L3V59_RS16060 (L3V59_16060) rpoN/rpoN1 3389868..3391373 (+) 1506 WP_122946298.1 RNA polymerase factor sigma-54 Machinery gene
  L3V59_RS16065 (L3V59_16065) raiA 3391532..3391891 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  L3V59_RS16070 (L3V59_16070) - 3392059..3392598 (+) 540 WP_122946770.1 PTS sugar transporter subunit IIA -
  L3V59_RS16075 (L3V59_16075) hprK 3392728..3393696 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  L3V59_RS16080 (L3V59_16080) rapZ 3393772..3394680 (+) 909 WP_043187318.1 RNase adapter RapZ -
  L3V59_RS16085 (L3V59_16085) - 3394708..3395343 (+) 636 WP_122946299.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54834.91 Da        Isoelectric Point: 4.8740

>NTDB_id=652999 L3V59_RS16060 WP_122946298.1 3389868..3391373(+) (rpoN/rpoN1) [Burkholderia aenigmatica strain CMCC(B)23010]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPTS
TSSSSSSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=652999 L3V59_RS16060 WP_122946298.1 3389868..3391373(+) (rpoN/rpoN1) [Burkholderia aenigmatica strain CMCC(B)23010]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACACCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGTGTCGCAGCGGACGGATCGTTGATCGCGCAGACGCCGCCCGCGCAGAACACCGAGCCCACTTCG
ACGAGCAGCAGCAGCTCGTCGAGCGATCGCGCCGAGCGCGACGAGCCGGCCGGCGCGGACGAATACGACGGTTACGGGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAATCTCGACGAATTCGGCCGCTCGAGCGGTGCATCTGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGTGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCGACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGGGTCGGTGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCGCGCGACTTCACGCGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACACGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAATCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTCAAGCAACA
GCTGCAGGAAGCACGCTGGCTGATCAAAAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCGATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCTTCGTCGACGGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCTAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGATTCGTGGTCGCGCGC
CGCACGGTTGCGAAGTACCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTACGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.921

100

0.685