Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   L5465_RS07880 Genome accession   NZ_CP091596
Coordinates   1524129..1524929 (-) Length   266 a.a.
NCBI ID   WP_001984465.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain Nord4-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1519129..1529929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L5465_RS07860 (L5465_07860) - 1520194..1520892 (-) 699 WP_000058788.1 YfgM family protein -
  L5465_RS07865 (L5465_07865) hisS 1520921..1522213 (-) 1293 WP_000095254.1 histidine--tRNA ligase -
  L5465_RS07870 (L5465_07870) ispG 1522210..1523325 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  L5465_RS07875 (L5465_07875) - 1523347..1524138 (-) 792 WP_000405161.1 helix-turn-helix domain-containing protein -
  L5465_RS07880 (L5465_07880) pilF 1524129..1524929 (-) 801 WP_001984465.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  L5465_RS07885 (L5465_07885) rlmN 1524945..1526177 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  L5465_RS07890 (L5465_07890) ndk 1526300..1526731 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  L5465_RS07895 (L5465_07895) iscX 1526845..1527045 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  L5465_RS07900 (L5465_07900) - 1527145..1527696 (-) 552 WP_000669565.1 phosphatase PAP2 family protein -
  L5465_RS07905 (L5465_07905) - 1527721..1529019 (-) 1299 WP_000348499.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30084.40 Da        Isoelectric Point: 9.2307

>NTDB_id=652637 L5465_RS07880 WP_001984465.1 1524129..1524929(-) (pilF) [Acinetobacter baumannii strain Nord4-2]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKSNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=652637 L5465_RS07880 WP_001984465.1 1524129..1524929(-) (pilF) [Acinetobacter baumannii strain Nord4-2]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAATCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(88-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722