Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LVJ79_RS04395 Genome accession   NZ_CP091518
Coordinates   825345..826721 (+) Length   458 a.a.
NCBI ID   WP_003785922.1    Uniprot ID   -
Organism   Kingella kingae strain 4177/66     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 820345..831721
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ79_RS04375 (LVJ79_04375) glnA 821210..822628 (+) 1419 WP_003785915.1 type I glutamate--ammonia ligase -
  LVJ79_RS04380 (LVJ79_04380) - 822745..823503 (+) 759 WP_003785916.1 ABC transporter ATP-binding protein -
  LVJ79_RS04385 (LVJ79_04385) proC 823539..824333 (-) 795 WP_003785917.1 pyrroline-5-carboxylate reductase -
  LVJ79_RS04390 (LVJ79_04390) - 824794..825042 (+) 249 WP_003789854.1 hypothetical protein -
  LVJ79_RS04395 (LVJ79_04395) radA 825345..826721 (+) 1377 WP_003785922.1 DNA repair protein RadA Machinery gene
  LVJ79_RS04400 (LVJ79_04400) - 826985..827323 (+) 339 WP_003785924.1 hypothetical protein -
  LVJ79_RS04405 (LVJ79_04405) - 827625..830396 (-) 2772 WP_003785925.1 class I SAM-dependent DNA methyltransferase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49417.12 Da        Isoelectric Point: 7.6114

>NTDB_id=651981 LVJ79_RS04395 WP_003785922.1 825345..826721(+) (radA) [Kingella kingae strain 4177/66]
MAKAPKIQFQCTECGGTTPKWVGKCPHCGEWNTLSEQLTAPEPKNARFQSWAAETTQVQELSKVTAMEVPREATGMGELD
RVLGGGLVDGAVILLGGDPGIGKSTLLLQTIALIAKKRKVLYVSGEESAQQVALRSQRLGLNADGVNLLAEIRLEAIQAA
LKQHEPSVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRIAKQMGIAMIFVGHVTKDGAIAGPRVLEHMVDTVLYFEG
DQHSNYRMIRAIKNRFGAANELGVFAMTETGLKGVSNPSAIFLASYRDDVAGSCVLVTQEGSRPLLVEIQALVDDAHGFT
PKRLTVGLEQNRLAMLLAVLNRHAGIACFDQDVFLNAVGGVKISEPAADLAVILAMLSSYRNKPLPEKMVAFGEIGLSGE
IRPVPRGQERLKEAEKLGFKRAIVPKANLPKNLKEFPSLKIQGVSSLQEAVNACRDWE

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=651981 LVJ79_RS04395 WP_003785922.1 825345..826721(+) (radA) [Kingella kingae strain 4177/66]
ATGGCAAAAGCCCCAAAAATCCAATTCCAATGCACCGAATGCGGTGGCACAACACCCAAATGGGTTGGCAAATGCCCGCA
TTGTGGCGAATGGAACACGCTTTCTGAACAGCTAACCGCACCCGAACCCAAAAACGCGCGTTTCCAATCGTGGGCGGCGG
AAACCACGCAAGTGCAAGAATTATCCAAAGTTACCGCCATGGAAGTCCCACGCGAAGCCACTGGCATGGGCGAACTTGAC
CGCGTGTTGGGCGGCGGTTTGGTGGACGGTGCGGTCATTTTGCTCGGTGGCGACCCAGGTATTGGCAAATCCACTTTGTT
GTTGCAAACCATTGCTTTAATAGCGAAAAAACGCAAAGTGCTGTATGTGTCGGGTGAGGAATCGGCGCAACAAGTGGCGT
TGCGTTCGCAGCGTTTGGGGCTGAATGCGGACGGCGTGAATTTGTTGGCGGAAATCCGTTTGGAGGCGATTCAGGCTGCC
TTAAAACAGCACGAGCCGTCTGTGGTCGTGATTGACTCGATTCAAACGATGTATTCTGACCAAATCACGTCCGCCCCTGG
CTCGGTGTCGCAGGTGCGCGAATGTGCGGCGCAACTGACGCGAATCGCCAAACAAATGGGCATTGCGATGATTTTTGTTG
GACACGTTACCAAAGACGGCGCGATTGCGGGTCCGCGCGTGTTGGAGCATATGGTGGACACGGTGCTGTATTTTGAGGGC
GATCAGCATTCCAATTACCGCATGATTCGGGCGATTAAAAACCGTTTTGGGGCGGCGAATGAATTGGGCGTGTTTGCGAT
GACGGAAACGGGCTTGAAAGGCGTTTCTAATCCGTCTGCGATTTTTTTGGCGAGCTATCGCGATGATGTGGCGGGTTCGT
GCGTGTTGGTTACGCAAGAGGGCAGTCGTCCGCTTTTGGTGGAAATTCAGGCACTTGTTGATGATGCGCACGGTTTCACG
CCGAAGCGTTTAACGGTGGGTTTGGAGCAAAACCGTCTTGCCATGTTGCTGGCGGTGCTGAATCGCCACGCTGGCATTGC
GTGTTTTGACCAAGATGTGTTTTTGAATGCGGTCGGCGGCGTGAAAATCAGCGAACCTGCGGCGGATTTGGCGGTAATTT
TGGCGATGTTGTCGAGCTATCGCAATAAGCCGCTGCCTGAAAAAATGGTGGCGTTTGGGGAAATTGGTTTGAGTGGCGAA
ATTCGTCCTGTGCCACGTGGGCAGGAGCGTTTGAAAGAAGCGGAGAAATTGGGCTTTAAACGGGCGATTGTGCCGAAAGC
GAATTTGCCGAAGAATTTAAAAGAGTTCCCAAGCCTGAAAATTCAAGGCGTAAGCAGCCTGCAAGAAGCGGTGAATGCTT
GTCGGGATTGGGAATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(361-428)

AAA domain (AAA_25)

(79-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.33

99.345

0.5
  radA Streptococcus mitis SK321

48.786

98.908

0.483
  radA Streptococcus pneumoniae Rx1

48.246

99.563

0.48
  radA Streptococcus pneumoniae D39

48.246

99.563

0.48
  radA Streptococcus pneumoniae R6

48.246

99.563

0.48
  radA Streptococcus pneumoniae TIGR4

48.246

99.563

0.48
  radA Streptococcus mitis NCTC 12261

48.115

98.472

0.474