Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   L2I54_RS20325 Genome accession   NZ_CP091476
Coordinates   3999916..4000350 (-) Length   144 a.a.
NCBI ID   WP_061457673.1    Uniprot ID   -
Organism   Bacillus cereus strain GSICC 30237     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3994916..4005350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2I54_RS20310 (L2I54_20250) - 3996092..3996649 (+) 558 WP_000062063.1 PadR family transcriptional regulator -
  L2I54_RS20315 (L2I54_20255) metE 3997163..3999451 (+) 2289 WP_001007609.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  L2I54_RS20320 (L2I54_20260) comJ 3999490..3999879 (-) 390 WP_000424030.1 competence protein ComJ -
  L2I54_RS20325 (L2I54_20265) nucA/comI 3999916..4000350 (-) 435 WP_061457673.1 DNA-entry nuclease Machinery gene
  L2I54_RS20330 (L2I54_20270) - 4000577..4001326 (+) 750 WP_000388471.1 DUF3967 domain-containing protein -
  L2I54_RS20335 (L2I54_20275) kinB 4001365..4002639 (-) 1275 WP_000420370.1 sporulation sensor histidine kinase KinB -
  L2I54_RS20340 (L2I54_20280) - 4002950..4003150 (+) 201 WP_000929280.1 hypothetical protein -
  L2I54_RS20345 (L2I54_20285) - 4003273..4004436 (+) 1164 WP_061457675.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16044.39 Da        Isoelectric Point: 8.4331

>NTDB_id=651597 L2I54_RS20325 WP_061457673.1 3999916..4000350(-) (nucA/comI) [Bacillus cereus strain GSICC 30237]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYSSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=651597 L2I54_RS20325 WP_061457673.1 3999916..4000350(-) (nucA/comI) [Bacillus cereus strain GSICC 30237]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATTCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.027

78.472

0.542