Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LZS83_RS00530 Genome accession   NZ_CP091444
Coordinates   97599..98975 (+) Length   458 a.a.
NCBI ID   WP_063222341.1    Uniprot ID   A0A2B0WJ33
Organism   Bacillus cereus strain MO2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92599..103975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZS83_RS00510 ctsR 92792..93253 (+) 462 WP_060629196.1 transcriptional regulator CtsR -
  LZS83_RS00515 - 93427..93975 (+) 549 WP_063222339.1 UvrB/UvrC motif-containing protein -
  LZS83_RS00520 - 93980..95044 (+) 1065 WP_063222340.1 protein arginine kinase -
  LZS83_RS00525 clpC 95067..97502 (+) 2436 WP_060629199.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  LZS83_RS00530 radA 97599..98975 (+) 1377 WP_063222341.1 DNA repair protein RadA Machinery gene
  LZS83_RS00535 disA 98979..100052 (+) 1074 WP_309259592.1 DNA integrity scanning diadenylate cyclase DisA -
  LZS83_RS00540 - 100213..101322 (+) 1110 WP_242256486.1 PIN/TRAM domain-containing protein -
  LZS83_RS00545 ispD 101339..102019 (+) 681 WP_309259593.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  LZS83_RS00550 ispF 102135..102611 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49872.57 Da        Isoelectric Point: 7.4250

>NTDB_id=651181 LZS83_RS00530 WP_063222341.1 97599..98975(+) (radA) [Bacillus cereus strain MO2]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVISSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=651181 LZS83_RS00530 WP_063222341.1 97599..98975(+) (radA) [Bacillus cereus strain MO2]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAGTCACCGAAATATATGGGGAAATGCCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAGCCGGTTATATCATCAAGGCGCCTTAATTATGCCAATGCGATTCAAA
CAGAAGTAACAAAGCCAAGACGTCTTACTGAAGTAGAAACAAAGTCTGAGGCGCGTATTGAAACAAAATTTCAAGAGTTT
AACCGTGTACTTGGAGGCGGGATTGTAGATGGGTCCTTAGTACTTATTGGTGGAGATCCTGGGATTGGAAAATCAACATT
GTTATTACAGATTTCATCACAATTAGCGGATTCTTCATATGATGTACTATACATATCGGGTGAGGAGTCAGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGTAATCTATTTGTTGTAGCAGAAACGGATTTACAGCGAATTGCA
GCACACATTGAAGAGATGAATCCAGCTTTTGTTGTTATTGATTCTATTCAAACGATACATTTACCTGAGGTGACGTCAGC
ACCGGGAAGCGTGGCACAAGTACGTGAATGTACAGCAGAATTAATGAAACTTGCAAAAACGAAAGGAATCCCTATTTTTA
TCGTCGGACATGTGACAAAAGAAGGTGCAATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGACCGTCATCATACATATCGTATTTTGCGAGCTGTGAAGAATCGTTTTGGTTCCACGAATGAAATGGGTATTTT
TGAAATGAAAGAACTTGGCCTTGCGGAAGTCTTAAACCCTTCTGAAATTTTCCTTGAGGAAAGACCTGTTGGGGTTGCAG
GATCAACAGTGGTAGCCTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCGACGGGAATTGATCATAACCGTGTATCACTTATTATGGCAGTGTTAGAAAAAAGAACAGG
TTTATTATTGCAAAATCAAGACGCATATTTAAAAGTAGCAGGTGGTTTGAAATTAGATGAACCAGCAATTGATTTAGCTG
TGGCTTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCAACCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCGGCTAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCGGATGGGATTGAGGTAGTAGGTGTATCTAATTTGGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(60-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B0WJ33

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631