Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   L3V45_RS08515 Genome accession   NZ_CP091421
Coordinates   1749047..1749481 (+) Length   144 a.a.
NCBI ID   WP_003154085.1    Uniprot ID   -
Organism   Bacillus sp. R45     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1744047..1754481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3V45_RS08510 (L3V45_08510) - 1747547..1748758 (-) 1212 WP_032857407.1 cytochrome P450 -
  L3V45_RS08515 (L3V45_08515) nucA/comI 1749047..1749481 (+) 435 WP_003154085.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  L3V45_RS08520 (L3V45_08520) - 1749541..1750296 (+) 756 WP_003154084.1 YoaK family protein -
  L3V45_RS08525 (L3V45_08525) - 1750330..1750692 (-) 363 WP_003154082.1 hypothetical protein -
  L3V45_RS08530 (L3V45_08530) - 1750884..1752212 (-) 1329 WP_236837349.1 S8 family peptidase -
  L3V45_RS08535 (L3V45_08535) - 1752392..1752625 (+) 234 WP_024085328.1 hypothetical protein -
  L3V45_RS08540 (L3V45_08540) - 1752881..1753588 (+) 708 WP_160249381.1 poly-gamma-glutamate hydrolase family protein -
  L3V45_RS08545 (L3V45_08545) - 1753649..1754101 (+) 453 WP_014305039.1 OsmC family protein -
  L3V45_RS08550 (L3V45_08550) - 1754115..1754468 (-) 354 WP_003154073.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15509.42 Da        Isoelectric Point: 6.8606

>NTDB_id=650938 L3V45_RS08515 WP_003154085.1 1749047..1749481(+) (nucA/comI) [Bacillus sp. R45]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAAHAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=650938 L3V45_RS08515 WP_003154085.1 1749047..1749481(+) (nucA/comI) [Bacillus sp. R45]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCATGCTGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACAGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAACAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTATGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCATCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGCTATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486