Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   L2Y28_RS19870 Genome accession   NZ_CP091319
Coordinates   4127138..4128571 (+) Length   477 a.a.
NCBI ID   WP_022649530.1    Uniprot ID   A0AAI9D3Q5
Organism   Enterobacter sp. JH25     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4122138..4133571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Y28_RS19840 - 4122705..4123682 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  L2Y28_RS19845 kdsD 4123696..4124682 (+) 987 WP_022649528.1 arabinose-5-phosphate isomerase KdsD -
  L2Y28_RS19850 kdsC 4124697..4125263 (+) 567 WP_017382729.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  L2Y28_RS19855 lptC 4125260..4125835 (+) 576 WP_022649529.1 LPS export ABC transporter periplasmic protein LptC -
  L2Y28_RS19860 lptA 4125804..4126358 (+) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  L2Y28_RS19865 lptB 4126365..4127090 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  L2Y28_RS19870 rpoN/rpoN1 4127138..4128571 (+) 1434 WP_022649530.1 RNA polymerase factor sigma-54 Machinery gene
  L2Y28_RS19875 hpf 4128594..4128881 (+) 288 WP_014171818.1 ribosome hibernation promoting factor -
  L2Y28_RS19880 ptsN 4128964..4129455 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  L2Y28_RS19885 rapZ 4129501..4130355 (+) 855 WP_003861870.1 RNase adapter RapZ -
  L2Y28_RS19890 npr 4130352..4130624 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  L2Y28_RS19895 mtgA 4130664..4131389 (-) 726 WP_022649531.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  L2Y28_RS19900 elbB 4131386..4132039 (-) 654 WP_022649532.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54128.02 Da        Isoelectric Point: 4.3409

>NTDB_id=649155 L2Y28_RS19870 WP_022649530.1 4127138..4128571(+) (rpoN/rpoN1) [Enterobacter sp. JH25]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPE
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISEHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=649155 L2Y28_RS19870 WP_022649530.1 4127138..4128571(+) (rpoN/rpoN1) [Enterobacter sp. JH25]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAACAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCTCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAG
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGTGCAGATTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACTACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTGGAGCTGACGCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTGGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTCCTGAAACGCGTTCAGCGTTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGAGCATCTGGATTTGCTGGCAAATCACGACTTCCGCACCCTGATGCGCGTCACGCGC
CTCAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCACTCGATCCGCGTCCGGGGCAATCTATCCAGACCAG
CGAACCGGAATACGTTATTCCTGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATTAAGAGTCTGGAAAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCATTCTTTGAGCAGGGCGAAGAATTTATGAAACCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACAGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGCCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48