Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   L2Y90_RS10615 Genome accession   NZ_CP091307
Coordinates   2299793..2301298 (-) Length   501 a.a.
NCBI ID   WP_258281039.1    Uniprot ID   -
Organism   Burkholderia pyrrocinia strain LWK2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2294793..2306298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Y90_RS10590 (L2Y90_10575) - 2295822..2296457 (-) 636 WP_174421242.1 LON peptidase substrate-binding domain-containing protein -
  L2Y90_RS10595 (L2Y90_10580) rapZ 2296485..2297393 (-) 909 WP_152862523.1 RNase adapter RapZ -
  L2Y90_RS10600 (L2Y90_10585) hprK 2297469..2298437 (-) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  L2Y90_RS10605 (L2Y90_10590) - 2298567..2299106 (-) 540 WP_375167705.1 PTS sugar transporter subunit IIA -
  L2Y90_RS10610 (L2Y90_10595) hpf 2299276..2299635 (-) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  L2Y90_RS10615 (L2Y90_10600) rpoN/rpoN1 2299793..2301298 (-) 1506 WP_258281039.1 RNA polymerase factor sigma-54 Machinery gene
  L2Y90_RS10620 (L2Y90_10605) lptB 2301459..2302235 (-) 777 WP_114178085.1 LPS export ABC transporter ATP-binding protein -
  L2Y90_RS10625 (L2Y90_10610) lptA 2302232..2302897 (-) 666 WP_152862517.1 lipopolysaccharide transport periplasmic protein LptA -
  L2Y90_RS10630 (L2Y90_10615) lptC 2302918..2303532 (-) 615 WP_258281040.1 LPS export ABC transporter periplasmic protein LptC -
  L2Y90_RS10635 (L2Y90_10620) - 2303536..2304072 (-) 537 WP_152862513.1 HAD family hydrolase -
  L2Y90_RS10640 (L2Y90_10625) kdsD 2304072..2305055 (-) 984 WP_152862511.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54740.88 Da        Isoelectric Point: 4.8740

>NTDB_id=649061 L2Y90_RS10615 WP_258281039.1 2299793..2301298(-) (rpoN/rpoN1) [Burkholderia pyrrocinia strain LWK2]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLESDDEWIASPLRVAADGSLIAQTPPGQNTEPAS
ANIGSSSGDRAERDEPAGADEYDGYASGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=649061 L2Y90_RS10615 WP_258281039.1 2299793..2301298(-) (rpoN/rpoN1) [Burkholderia pyrrocinia strain LWK2]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAGCGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACACCTCCCGGCCAGAACACCGAGCCGGCCTCG
GCAAACATCGGCAGTTCGTCGGGCGATCGCGCGGAGCGCGACGAGCCGGCCGGCGCCGACGAATACGACGGTTATGCGTC
CGGCGACGGCAACGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCTCGAGCGGCGCGTCCGACGACGACGATCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGTGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGCGCGCGCTCGGCCTCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTGGAACTGCTCGCCGCACGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCATGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGTCGCGGCGATCCGTCGACCGGTTCGCTGAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAAAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATTGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.379

100

0.691