Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   L3I90_RS02045 Genome accession   NZ_CP091284
Coordinates   475409..477427 (+) Length   672 a.a.
NCBI ID   WP_057178780.1    Uniprot ID   A0A0R0M339
Organism   Cylindrospermopsis raciborskii KLL07     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470409..482427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3I90_RS02015 (L3I90_02015) - 470913..471461 (+) 549 WP_006276277.1 NYN domain-containing protein -
  L3I90_RS02025 (L3I90_02025) rsgA 471643..472701 (-) 1059 WP_006276278.1 small ribosomal subunit biogenesis GTPase RsgA -
  L3I90_RS02030 (L3I90_02030) - 472727..472969 (-) 243 WP_057178779.1 sulfurtransferase TusA family protein -
  L3I90_RS02035 (L3I90_02035) dnaJ 472979..474109 (-) 1131 WP_006276280.1 molecular chaperone DnaJ -
  L3I90_RS02040 (L3I90_02040) grpE 474394..475089 (-) 696 WP_369806407.1 nucleotide exchange factor GrpE -
  L3I90_RS02045 (L3I90_02045) pilB/pilB1 475409..477427 (+) 2019 WP_057178780.1 GspE/PulE family protein Machinery gene
  L3I90_RS02050 (L3I90_02050) pilT 477445..478521 (+) 1077 WP_115539360.1 type IV pilus twitching motility protein PilT Machinery gene
  L3I90_RS02055 (L3I90_02055) - 478564..479823 (+) 1260 WP_057177258.1 type II secretion system F family protein -
  L3I90_RS02060 (L3I90_02060) - 479852..480856 (-) 1005 WP_115539368.1 saccharopine dehydrogenase-like oxidoreductase -

Sequence

Protein


Download         Length: 672 a.a.        Molecular weight: 75595.36 Da        Isoelectric Point: 5.5115

>NTDB_id=648841 L3I90_RS02045 WP_057178780.1 475409..477427(+) (pilB/pilB1) [Cylindrospermopsis raciborskii KLL07]
MTYSVKKRLDKTSASQTNTLPVLNNLVNKLVKSGHIHREQMKLAVMESLESGKVLTDVLQSIARGKLDPQLIRECKKQHL
FELKILYGVEYIDPEVSQMNWNIIRELTESLIPIDICRSYGLLPLGKEENQNPPQVLVAMVEPDNLAACDYLNRILQPPN
WQFKRLVIAAEDYEILINQYTDEVIKEAREKEQTQFTDISQDLESLSLEVMEQRLEEGDLDLTVAMREAQDEPIINLVNK
ILFLALRDSASDIHVEPQEEYLRIRFRNDGVLRESESIPPIPKHIIPAVTARIKIISNLDIAEKRLPQDGRIRRVFDGRK
VDIRVSTLPNRYGEKICLRILDNSSTKLPLDQIITDPHTLQVFKNIIHKPLGLILVTGPTGSGKTTSLYSALAELNDPGI
NISTVEDPVEYSLPGLTQVQVIREKGLDFTTVLRAFLRQDPDVLLVGETRDGETAKTAIEAALTGHLVFTTLHTIDAAGA
IARLLEMGIEPFMISSSLIGVLAQRLVRRLCPTCRIPYTPTVQELGRYGLSLAQEGNVIFYKANTISSELIGKSPNKIKI
CPNCYGTGYKGRCGVYEVMTITQKIQSLINQEADTAKIKEAALEEGMITLLGYSLNLVRQGITTLEEVERVVYTDRELKV
SRQNSLTCQCCDATLKPEWLDCPYCLTPRLIN

Nucleotide


Download         Length: 2019 bp        

>NTDB_id=648841 L3I90_RS02045 WP_057178780.1 475409..477427(+) (pilB/pilB1) [Cylindrospermopsis raciborskii KLL07]
ATGACTTACTCGGTTAAAAAACGTTTAGACAAAACTAGTGCTAGTCAAACTAACACTCTTCCCGTACTGAACAACTTAGT
AAACAAACTAGTTAAATCTGGTCATATTCATAGGGAACAGATGAAACTGGCCGTGATGGAAAGTCTTGAATCTGGGAAAG
TATTAACAGATGTTTTACAATCTATAGCCAGAGGGAAGTTAGATCCCCAGTTAATCAGAGAATGTAAAAAACAGCATTTA
TTTGAATTAAAAATACTATATGGTGTAGAATATATAGACCCAGAAGTCAGTCAAATGAACTGGAATATTATCAGAGAATT
GACCGAGAGTTTAATACCAATAGATATTTGTCGTAGTTATGGATTATTACCATTAGGAAAGGAAGAAAATCAGAATCCAC
CCCAGGTGTTAGTAGCAATGGTTGAGCCAGATAATTTAGCAGCATGTGATTACCTTAATAGGATATTACAACCGCCAAAC
TGGCAGTTTAAACGTCTAGTAATTGCCGCAGAAGATTACGAAATATTAATTAATCAATACACAGATGAAGTAATCAAAGA
AGCAAGAGAAAAAGAGCAAACTCAATTCACAGATATTAGCCAAGACCTAGAAAGTTTAAGTCTTGAGGTTATGGAGCAGA
GACTAGAAGAAGGAGATCTAGATTTGACAGTAGCCATGAGAGAAGCTCAAGATGAGCCAATTATTAATCTTGTGAATAAA
ATTCTCTTTTTAGCCCTGCGAGATAGTGCTTCAGATATTCACGTTGAACCTCAAGAAGAATATTTACGAATTCGGTTTCG
CAACGATGGGGTACTGCGGGAATCGGAATCTATTCCCCCCATTCCCAAACATATTATTCCTGCAGTTACCGCGCGAATTA
AAATTATCTCCAATCTAGATATCGCCGAAAAAAGATTGCCCCAAGATGGCAGAATTCGCAGAGTATTTGATGGTAGAAAG
GTAGATATCCGAGTCAGCACCTTACCCAACCGCTACGGAGAAAAAATCTGTCTGAGGATTTTAGACAATTCCTCCACTAA
ATTACCCCTAGATCAGATAATTACTGATCCCCATACTTTGCAGGTATTTAAAAATATTATTCATAAACCCCTAGGTTTAA
TTTTAGTTACAGGTCCAACCGGTTCTGGAAAAACTACCTCCTTATATTCTGCTCTAGCAGAATTAAATGATCCGGGAATT
AATATTAGTACAGTTGAAGATCCCGTAGAATACAGTTTACCGGGACTGACTCAAGTACAGGTAATCAGAGAAAAGGGATT
AGACTTTACCACAGTTCTGCGAGCTTTTCTGCGACAAGACCCAGACGTGTTATTGGTGGGAGAAACACGAGATGGGGAAA
CTGCGAAAACTGCAATTGAAGCAGCACTAACCGGACATCTAGTATTCACTACCTTGCATACTATAGATGCTGCAGGAGCG
ATCGCCCGCTTGCTAGAAATGGGTATTGAACCATTTATGATTTCTAGTTCTCTAATTGGTGTATTAGCCCAGAGGTTAGT
GCGTAGACTGTGTCCCACATGTCGAATTCCCTATACTCCTACAGTTCAAGAACTAGGGAGATATGGTTTATCCCTAGCGC
AAGAAGGCAATGTCATTTTCTATAAAGCTAATACCATATCATCAGAACTAATAGGAAAAAGCCCAAATAAAATCAAAATT
TGTCCTAACTGTTATGGTACTGGCTACAAAGGGCGTTGTGGAGTGTATGAAGTTATGACAATTACGCAAAAAATACAAAG
TTTAATTAATCAAGAAGCTGACACAGCCAAAATTAAAGAAGCAGCTTTAGAAGAGGGAATGATAACCCTATTGGGTTATA
GTCTCAATCTAGTTCGTCAAGGTATAACGACCTTAGAAGAAGTGGAGCGGGTGGTATATACAGATAGGGAATTAAAAGTC
TCACGACAGAATAGTCTCACATGTCAATGTTGTGATGCTACCCTAAAACCAGAGTGGTTAGATTGTCCTTATTGTTTGAC
CCCCAGATTAATTAATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(76-182)

Type II/IV secretion system protein (T2SSE)

(240-509)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0R0M339

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

55.505

97.321

0.54