Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   L2Z53_RS11280 Genome accession   NZ_CP091281
Coordinates   2185544..2186905 (-) Length   453 a.a.
NCBI ID   WP_086043531.1    Uniprot ID   A0A1W7AEG9
Organism   Macrococcoides canis strain B8     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2180544..2191905
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Z53_RS11255 (L2Z53_11255) cysE 2180969..2181586 (-) 618 WP_407944100.1 serine O-acetyltransferase -
  L2Z53_RS11260 (L2Z53_11260) gltX 2181861..2183318 (-) 1458 WP_086043689.1 glutamate--tRNA ligase -
  L2Z53_RS11265 (L2Z53_11265) ispF 2183333..2183812 (-) 480 WP_086043528.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  L2Z53_RS11270 (L2Z53_11270) ispD 2183809..2184480 (-) 672 WP_331474511.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  L2Z53_RS11275 (L2Z53_11275) - 2184482..2185531 (-) 1050 WP_086043530.1 PIN/TRAM domain-containing protein -
  L2Z53_RS11280 (L2Z53_11280) radA 2185544..2186905 (-) 1362 WP_086043531.1 DNA repair protein RadA Machinery gene
  L2Z53_RS11285 (L2Z53_11285) - 2187090..2189516 (-) 2427 WP_086043532.1 ATP-dependent Clp protease ATP-binding subunit -
  L2Z53_RS11290 (L2Z53_11290) - 2189526..2190530 (-) 1005 WP_086043533.1 protein arginine kinase -
  L2Z53_RS11295 (L2Z53_11295) - 2190520..2190906 (-) 387 WP_157891126.1 UvrB/UvrC motif-containing protein -
  L2Z53_RS11300 (L2Z53_11300) - 2190917..2191378 (-) 462 WP_086043535.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50181.81 Da        Isoelectric Point: 7.2378

>NTDB_id=648835 L2Z53_RS11280 WP_086043531.1 2185544..2186905(-) (radA) [Macrococcoides canis strain B8]
MAKVKSTFECMACGYQSPKWMGKCPNCGAWNQMEEVIEHKQKGPKNAISESQSDNKVEKLKDITKESVPRDHTQMKELDR
VLGGGIVPGSLILIGGDPGIGKSTLLLQVCAMLSQNHPVLYISGEESVRQTKLRADRLLEDAGELDVYAETNLQIIHETV
KKTKPKFLVIDSIQTIFHPEVTSAPGSVSQVRECTQELMRIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
THHSYRILRAVKNRFGSTNEMGIFEMKNTGLKEVLNPSEMFLEERTKNVAGSTIVATMEGTRPLLVEVQSLVTPTSFHNP
RRMASGVDHNRLSLLMAVLEKKQGYLLQQQDAYVKVAGGVKLDEPAVDLSVIVSIASSYNDKPTRGDDCFIGEVGLTGEV
RRVARIEQRVQEAEKLGFKRVIIPKNNIGGWDFPGNIEVIGVTNINEALKIAF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=648835 L2Z53_RS11280 WP_086043531.1 2185544..2186905(-) (radA) [Macrococcoides canis strain B8]
TTGGCAAAGGTGAAAAGTACATTTGAATGTATGGCCTGTGGTTATCAGTCACCGAAATGGATGGGGAAATGTCCGAATTG
TGGTGCATGGAATCAGATGGAAGAAGTTATTGAACATAAACAGAAAGGCCCTAAAAATGCGATTTCTGAATCACAAAGTG
ATAATAAAGTTGAAAAGTTAAAAGATATAACAAAAGAAAGTGTCCCTCGAGATCATACGCAGATGAAAGAACTGGATCGT
GTACTTGGAGGAGGAATTGTTCCGGGTTCATTAATACTTATCGGAGGAGACCCTGGAATTGGGAAATCTACATTGCTGCT
GCAAGTTTGTGCAATGCTTTCTCAAAACCATCCTGTACTTTATATTTCCGGAGAGGAATCCGTAAGACAGACAAAACTAC
GAGCCGATCGTCTTTTAGAGGATGCAGGTGAGCTTGATGTCTATGCAGAAACAAACCTTCAAATTATACACGAAACGGTA
AAGAAAACAAAACCAAAATTTTTAGTTATCGATTCAATTCAAACGATATTTCATCCAGAAGTTACGAGTGCACCGGGTTC
TGTATCGCAAGTCAGAGAATGTACTCAAGAATTAATGCGTATCGCAAAGCAGATGAATATCGCCACATTTATCGTAGGGC
ATGTGACGAAAGAGGGTCAGATTGCAGGTCCAAGATTACTTGAACATATGGTTGATACAGTCCTTTACTTTGAAGGAGAT
ACACATCACAGCTACAGAATATTACGTGCAGTGAAGAATCGTTTTGGTTCAACAAACGAAATGGGTATATTTGAAATGAA
GAATACAGGTCTTAAAGAAGTTCTGAATCCTTCAGAAATGTTCCTTGAAGAACGTACAAAAAATGTAGCGGGTTCGACTA
TTGTTGCAACGATGGAAGGGACGCGACCATTATTGGTTGAAGTTCAGTCGCTTGTAACGCCGACATCCTTTCACAATCCA
AGAAGGATGGCGTCAGGTGTCGATCATAATCGACTGAGTTTACTGATGGCTGTATTGGAGAAAAAACAAGGGTATTTATT
ACAGCAGCAAGATGCATACGTGAAAGTCGCGGGCGGAGTGAAACTTGATGAACCTGCTGTTGATTTAAGTGTTATCGTAA
GTATCGCATCAAGTTATAATGATAAGCCGACACGTGGAGATGACTGCTTTATCGGAGAGGTCGGTCTGACAGGTGAGGTA
AGACGTGTTGCACGTATAGAACAGCGCGTACAAGAGGCTGAGAAGCTCGGTTTTAAACGTGTGATCATCCCTAAAAATAA
TATTGGTGGCTGGGATTTCCCTGGAAATATTGAAGTGATTGGTGTAACAAATATAAACGAAGCATTGAAAATTGCATTTT
AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(343-427)

AAA domain (AAA_25)

(66-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W7AEG9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.667

100

0.671
  radA Streptococcus pneumoniae Rx1

62.996

100

0.631
  radA Streptococcus pneumoniae D39

62.996

100

0.631
  radA Streptococcus pneumoniae R6

62.996

100

0.631
  radA Streptococcus pneumoniae TIGR4

62.996

100

0.631
  radA Streptococcus mitis NCTC 12261

62.996

100

0.631
  radA Streptococcus mitis SK321

62.996

100

0.631