Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   H7R02_RS01915 Genome accession   NZ_AP021963
Coordinates   429006..429683 (-) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli strain WP3-W18-CRE-03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 424006..434683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R02_RS01900 (WP3W18C03_03800) chiA 425364..428066 (+) 2703 WP_000773146.1 bifunctional chitinase/lysozyme -
  H7R02_RS01905 (WP3W18C03_03810) bfd 428235..428429 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  H7R02_RS01910 (WP3W18C03_03820) bfr 428501..428977 (+) 477 WP_000675504.1 bacterioferritin -
  H7R02_RS01915 (WP3W18C03_03830) pilD 429006..429683 (-) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  H7R02_RS01920 (WP3W18C03_03840) gspM 429683..430144 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  H7R02_RS01925 (WP3W18C03_03850) gspL 430141..431304 (-) 1164 Protein_387 type II secretion system protein GspL -
  H7R02_RS01930 (WP3W18C03_03860) gspK 431319..432302 (-) 984 WP_001058513.1 type II secretion system minor pseudopilin GspK -
  H7R02_RS01935 (WP3W18C03_03870) gspJ 432295..432882 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  H7R02_RS01940 (WP3W18C03_03880) gspI 432875..433252 (-) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  H7R02_RS01945 (WP3W18C03_03890) gspH 433249..433758 (-) 510 WP_001539909.1 type II secretion system minor pseudopilin GspH -
  H7R02_RS01950 (WP3W18C03_03900) gspG 433766..434203 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=64859 H7R02_RS01915 WP_000178175.1 429006..429683(-) (pilD) [Escherichia coli strain WP3-W18-CRE-03]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=64859 H7R02_RS01915 WP_000178175.1 429006..429683(-) (pilD) [Escherichia coli strain WP3-W18-CRE-03]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387

Multiple sequence alignment