Detailed information NTG0648269

Bioinformatically predicted

Overview

• Name: pepF
• Type: Regulator
• Locus tag: L2629_RS03240
• Genome accession: NZ_CP091198
• Coordinates: 573567..575378 (-)
• Length: 603 a.a.
• NCBI ID: WP_002387169.1
• Uniprot ID: A0A855UCR5
• Organism: Enterococcus faecalis strain UK045
• Function: degradation of XIP; competence shut-off (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
ICE	566507..585865	573567..575378	within	0

Gene organization within MGE regions

Location: 566507..585865

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L2629_RS03205 (L2629_03190)	cutC	567453..568082 (+)	630	WP_002356490.1	copper homeostasis protein CutC	-
L2629_RS03210 (L2629_03195)	mgtE	568200..569561 (-)	1362	WP_002362548.1	magnesium transporter	-
L2629_RS03215 (L2629_03200)	-	569592..570485 (-)	894	WP_002356487.1	RluA family pseudouridine synthase	-
L2629_RS03220 (L2629_03205)	-	570493..571290 (-)	798	WP_002356486.1	NAD kinase	-
L2629_RS03225 (L2629_03210)	-	571303..571977 (-)	675	WP_002356484.1	GTP pyrophosphokinase family protein	-
L2629_RS03230 (L2629_03215)	-	572174..572752 (+)	579	WP_002356483.1	CYTH domain-containing protein	-
L2629_RS03235 (L2629_03220)	-	572816..573475 (+)	660	WP_002368840.1	ClpXP adapter SpxH family protein	-
L2629_RS03240 (L2629_03225)	pepF	573567..575378 (-)	1812	WP_002387169.1	oligoendopeptidase F	Regulator
L2629_RS03245 (L2629_03230)	-	575426..576604 (-)	1179	WP_002387170.1	competence protein CoiA	-
L2629_RS03250 (L2629_03235)	-	576694..577356 (-)	663	WP_002356478.1	adaptor protein MecA	-
L2629_RS03255 (L2629_03240)	spxA	577698..578096 (-)	399	WP_002356477.1	transcriptional regulator SpxA	-
L2629_RS03260 (L2629_03245)	dfrF	578566..579060 (-)	495	WP_002322211.1	trimethoprim-resistant dihydrofolate reductase DfrF	-
L2629_RS03265 (L2629_03250)	-	579368..579847 (-)	480	WP_002322212.1	MGMT family protein	-
L2629_RS03270	-	580006..580146 (-)	141	WP_171042677.1	hypothetical protein	-
L2629_RS03275 (L2629_03255)	-	580158..580829 (-)	672	WP_002322214.1	helix-turn-helix domain-containing protein	-
L2629_RS03280 (L2629_03260)	-	581423..582433 (+)	1011	WP_009274733.1	replication initiation factor domain-containing protein	-
L2629_RS03285 (L2629_03265)	-	582433..582636 (+)	204	WP_002607397.1	excisionase	-
L2629_RS03290 (L2629_03270)	-	582715..583908 (+)	1194	WP_010706472.1	site-specific integrase	-
L2629_RS03295 (L2629_03275)	trpS	584262..585272 (+)	1011	WP_002378619.1	tryptophan--tRNA ligase	-

Sequence

Protein

• Length: 603 a.a.
• Molecular weight: 68848.16 Da
• Isoelectric Point: 4.6861

>NTDB_id=648269 L2629_RS03240 WP_002387169.1 573567..575378(-) (pepF) [Enterococcus faecalis strain UK045]
MSEIKQLPTRDEVPTPLTWDLTKIFKDDAAFDVAYNQLIEELNQAESFKGTLGDGAEAFLAALEYVLDVYRKVETLYVYS
HLKNDQDTTNTAYQALYARASSLYAQVSEAVSWFDPEVLTLSDEQIWGYFEEQPKLAVYRHYIQNILDERPHVLSMEQEA
LLAGASEIFGASSNTFSILNNADLEFPTVQNAEGETIQLSHGVYGQLMESVDPSVREAAFKGLYKVYKQFRNTLASTLGA
HVKTHNYKAKIRNYDSARAASLASNHIPESVHETLVAVVNKHLPLLHRYVKLRKKLLNVEELHMYDLYAPLLGEAPIRYS
YEEAKEKAIEALKPLGEDYLSIVKEAFSSRWIDVIENQGKRSGAYSSGAYDTAPYILMNWHDSLDQLFTLVHEMGHSVHS
YYTRNNQPYVYGDYSIFLAEIASTTNENILTEYLLQTETDPKVRAYVLNHYLDGFKGTIFRQTQFAEFEHFIHTEDAKGT
PLTSEYLSEYYGELNAKYYGPEVVRDEEISYEWARIPHFYYNYYVYQYATGFSAASALSKHILAGEEGALENYLNYLKAG
SSDFPIEVMKKAGVDMTQAAYIEDAMKVFEERLTELEALVEKL

Nucleotide

• Length: 1812 bp

>NTDB_id=648269 L2629_RS03240 WP_002387169.1 573567..575378(-) (pepF) [Enterococcus faecalis strain UK045]
ATGAGCGAAATTAAACAATTACCAACACGTGATGAAGTTCCAACTCCATTGACTTGGGATTTAACCAAAATTTTTAAAGA
TGATGCTGCTTTTGATGTTGCGTATAATCAGTTGATAGAAGAGCTAAATCAAGCAGAATCATTTAAAGGTACCTTAGGGG
ATGGTGCAGAAGCTTTTCTAGCTGCGTTAGAATACGTTTTAGATGTCTATCGAAAAGTGGAAACATTATATGTCTATTCT
CATTTAAAAAATGATCAAGACACTACAAATACAGCTTACCAAGCTTTATATGCCAGAGCTAGTTCTTTATATGCACAAGT
TAGTGAAGCTGTTTCTTGGTTTGACCCAGAGGTTTTAACATTAAGTGATGAACAAATCTGGGGATATTTTGAAGAGCAGC
CGAAATTAGCTGTTTATCGTCACTATATTCAAAATATTTTAGATGAACGTCCCCATGTTTTATCGATGGAACAAGAAGCT
TTACTGGCGGGGGCTAGTGAAATTTTTGGTGCCTCAAGTAATACATTTTCAATTTTAAATAATGCAGACTTAGAATTTCC
AACTGTGCAAAATGCTGAAGGCGAAACGATTCAACTTTCTCATGGCGTTTATGGTCAGTTAATGGAAAGTGTCGATCCAT
CTGTTCGTGAAGCAGCATTTAAAGGTTTGTACAAAGTATACAAACAATTTAGAAATACATTAGCCTCAACTTTAGGTGCA
CATGTTAAAACACATAATTATAAAGCAAAAATTAGAAATTATGATTCTGCTCGAGCAGCCTCTTTAGCAAGTAATCATAT
CCCTGAAAGTGTTCACGAAACATTAGTAGCTGTAGTAAATAAACATTTACCTTTGCTACATCGTTATGTAAAATTAAGAA
AAAAATTATTAAACGTAGAAGAATTACACATGTACGACTTGTATGCGCCTTTGCTAGGTGAAGCACCAATTCGTTACAGC
TATGAAGAAGCAAAAGAAAAAGCAATTGAGGCTTTAAAACCACTAGGTGAAGACTATTTATCTATTGTTAAAGAAGCTTT
TTCAAGTCGCTGGATTGATGTGATTGAAAATCAAGGAAAACGAAGCGGCGCATATTCTTCAGGAGCTTATGATACAGCCC
CATACATTTTAATGAATTGGCATGATAGTTTGGATCAACTATTTACATTAGTCCATGAGATGGGCCATAGTGTTCATAGT
TACTATACAAGAAATAATCAGCCGTATGTTTATGGCGACTATTCAATTTTCTTAGCTGAGATTGCTTCAACCACAAATGA
AAATATTTTAACAGAATATTTATTACAAACAGAAACAGATCCTAAAGTACGTGCGTATGTCTTAAATCACTATTTAGACG
GCTTTAAGGGAACCATTTTCCGTCAAACGCAATTTGCGGAATTTGAACATTTTATTCACACGGAAGATGCTAAAGGCACG
CCATTAACAAGTGAATATTTGAGTGAGTATTATGGCGAGCTCAATGCTAAATATTATGGACCAGAAGTAGTTAGAGACGA
AGAAATCAGTTACGAATGGGCGAGAATTCCACATTTTTATTACAATTACTATGTTTATCAATATGCAACTGGCTTCTCAG
CCGCTTCGGCATTGTCTAAACATATTTTAGCTGGAGAAGAGGGAGCTTTAGAGAATTATCTTAACTACTTGAAAGCAGGA
AGTAGTGACTTCCCAATTGAAGTGATGAAAAAAGCGGGCGTGGATATGACACAAGCCGCGTATATCGAAGATGCAATGAA
AGTTTTTGAAGAACGTTTAACGGAATTAGAAGCTTTGGTTGAAAAATTATAG

Domains

Predicted by InterproScan.

• Peptidase family M3 (Peptidase_M3) (207-587)

• Oligopeptidase F (Peptidase_M3_N) (117-186)

3D structure

• AlphaFold DB: A0A855UCR5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pepF	Streptococcus salivarius strain HSISS4	54.806	98.342	0.539