Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   L1871_RS20225 Genome accession   NZ_CP091176
Coordinates   4271981..4273420 (-) Length   479 a.a.
NCBI ID   WP_041211850.1    Uniprot ID   A0A2L0TRY9
Organism   Aeromonas caviae strain SCLZS52     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4266981..4278420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1871_RS20195 (L1871_20190) dagF 4267260..4268000 (+) 741 WP_236275443.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  L1871_RS20200 (L1871_20195) - 4268103..4270008 (+) 1906 Protein_3907 BglG family transcription antiterminator -
  L1871_RS20205 (L1871_20200) npr 4270024..4270296 (-) 273 WP_010675868.1 PTS phosphocarrier protein NPr -
  L1871_RS20210 (L1871_20205) rapZ 4270317..4271183 (-) 867 WP_236275444.1 RNase adapter RapZ -
  L1871_RS20215 (L1871_20210) ptsN 4271224..4271670 (-) 447 WP_010675870.1 PTS IIA-like nitrogen regulatory protein PtsN -
  L1871_RS20220 (L1871_20215) hpf 4271673..4271960 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  L1871_RS20225 (L1871_20220) rpoN/rpoN1 4271981..4273420 (-) 1440 WP_041211850.1 RNA polymerase factor sigma-54 Machinery gene
  L1871_RS20230 (L1871_20225) lptB 4273486..4274211 (-) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  L1871_RS20235 (L1871_20230) lptA 4274224..4274742 (-) 519 WP_010675873.1 lipopolysaccharide transport periplasmic protein LptA -
  L1871_RS20240 (L1871_20235) lptC 4274729..4275289 (-) 561 WP_010675874.1 LPS export ABC transporter periplasmic protein LptC -
  L1871_RS20245 (L1871_20240) kdsC 4275286..4275840 (-) 555 WP_103259443.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  L1871_RS20250 (L1871_20245) - 4275840..4276835 (-) 996 WP_029314845.1 KpsF/GutQ family sugar-phosphate isomerase -
  L1871_RS20255 (L1871_20250) mlaF 4277042..4277842 (+) 801 WP_039040471.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53731.54 Da        Isoelectric Point: 4.3728

>NTDB_id=648199 L1871_RS20225 WP_041211850.1 4271981..4273420(-) (rpoN/rpoN1) [Aeromonas caviae strain SCLZS52]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAQETSQAETQDASQLDSSDAMAQESMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTATLED
IQAAVSSEEVEVELDEIEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLDEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=648199 L1871_RS20225 WP_041211850.1 4271981..4273420(-) (rpoN/rpoN1) [Aeromonas caviae strain SCLZS52]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAGAACGAGG
CGCAGGAAACCAGTCAGGCAGAGACACAGGACGCCAGTCAACTCGACTCCAGCGATGCCATGGCCCAGGAGAGCATGCCG
GATGAACTGCCGGTGGACACCACCTGGGACGAGGTCTACTCGGCCGGTACAGCTCAGAGTGCAGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAGACCACCGAGAACCTGCAAGACTACCTGCTGTGGCAGATGCGGCTCACCCCGTTCA
GTGATGTGGATGCTGCCATCGCGCTTGCCATCATCGATGCCATCGACGAATCCGGGTATCTGACTGCCACCCTCGAGGAT
ATTCAGGCTGCAGTCAGCAGCGAGGAGGTAGAAGTCGAACTGGATGAGATCGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCCCGTTCGGTGCAAGAGTGTCTGCTGATCCAGCTCGGCCAGTATTCGCCGGACCTGCCCT
GGCTCGACGAAGCCAGAGAGGTGATCCGCGAACACATGGACCTGCTCGGCAATCGCGACTACCGCACCCTGGCTCGCAAG
ACCCGCCTCAAGGAGAGCGATCTCAAGGAGGTGCTGGATCTCATCCAGCAACTGGAGCCCAGACCCGGCAACGGCATCAT
CCAGAGTGAATCCCAGTACGTGATCCCGGATGTGTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTTGAACTCAATCCGG
ACTCTGCTCCGCGCATCCGTGTCAACGAGGCCTATGCGGCCATGGCGCGCAATGCTCGCAGCAGCACGGATACCCAGTTC
ATCCGTTCTCATTTGCAGGAGGCCAAGTGGTTCATCAAGAGCCTGGAGAGTCGTAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTTGAGCAGCAGCAGGGCTTTTTCGAGTACGGCGAAGAGGCCATGAAACCCATGGTACTCAACGACATCGCGG
AAGCGGTGGAGATGCATGAGTCGACCATTTCACGGGTCACGACCCAGAAGTACATGCATACGCCCCGCGGCATTTTTGAA
CTGAAGTTTTTTTTCTCCAGTCACGTCAATACGGAGGGAGGAGGAGAGTGCTCATCGACCGCTATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCGGAAAACCCCGCCAAGCCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0TRY9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.472

100

0.482