Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   L2Y00_RS17715 Genome accession   NZ_CP091143
Coordinates   3956104..3958209 (-) Length   701 a.a.
NCBI ID   WP_005621204.1    Uniprot ID   A0A3M5TXK6
Organism   Pseudomonas avellanae strain NCPPB 3491     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3951104..3963209
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Y00_RS17700 (L2Y00_17700) - 3952729..3954348 (-) 1620 WP_003378845.1 AAA family ATPase -
  L2Y00_RS17705 (L2Y00_17705) aroB 3954359..3955462 (-) 1104 WP_005621200.1 3-dehydroquinate synthase -
  L2Y00_RS17710 (L2Y00_17710) aroK 3955581..3956099 (-) 519 WP_003378847.1 shikimate kinase AroK -
  L2Y00_RS17715 (L2Y00_17715) pilQ 3956104..3958209 (-) 2106 WP_005621204.1 type IV pilus secretin PilQ Machinery gene
  L2Y00_RS17720 (L2Y00_17720) pilP 3958257..3958784 (-) 528 WP_003378849.1 type 4a pilus biogenesis lipoprotein PilP -
  L2Y00_RS17725 (L2Y00_17725) pilO 3958781..3959404 (-) 624 WP_003378850.1 type 4a pilus biogenesis protein PilO -
  L2Y00_RS17730 (L2Y00_17730) - 3959401..3960027 (-) 627 WP_005621206.1 PilN domain-containing protein -
  L2Y00_RS17735 (L2Y00_17735) pilM 3960027..3961091 (-) 1065 WP_003378852.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75444.88 Da        Isoelectric Point: 4.9249

>NTDB_id=648008 L2Y00_RS17715 WP_005621204.1 3956104..3958209(-) (pilQ) [Pseudomonas avellanae strain NCPPB 3491]
MTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTEQPARIALDLPGVTSQLETKSRE
LGTGNARSVVVVQAQDRTRVIINLTTLAPYSSRVDGNNLFVVIGQGAAAAQASQPTTAMGAQRVAVPPPVPASAKPFIPA
GVKAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASATGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKAEERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGSG
KWTTYGLDDNGDEAGNTSGNLTSNVPFVDLGAAGATSGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=648008 L2Y00_RS17715 WP_005621204.1 3956104..3958209(-) (pilQ) [Pseudomonas avellanae strain NCPPB 3491]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATAGCGATGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCAGGCGATCGCATCGAGCTCAAGCTCGCCTTTGACGCACCGGTGCCCGCGCCGCGCG
GCTACACCACCGAGCAGCCGGCGCGCATCGCTCTGGACCTGCCGGGCGTGACCAGCCAGTTGGAGACCAAAAGCCGTGAG
CTGGGCACCGGCAATGCACGCAGCGTAGTGGTGGTGCAGGCTCAGGATCGCACGCGAGTGATCATCAACCTGACGACGCT
TGCGCCTTACAGCTCCCGCGTCGACGGCAACAATCTGTTCGTGGTCATTGGCCAGGGTGCCGCCGCAGCGCAGGCTTCCC
AGCCAACCACTGCGATGGGCGCGCAACGGGTAGCGGTGCCGCCGCCTGTCCCGGCGTCTGCCAAGCCCTTTATACCGGCG
GGCGTCAAAGCCATCCGCAATGTCGATTTTCAGCGCGGTGAGCTGGGTGAAGGCAATGTGGTCATCGACCTGAGTAACCC
GAGCATCGCGCCTGATATTCAGGAGCAGGGCGGCAAGATCCGTGTCGACTTCGCCAAGACCCAGTTGCCCGAGCCTCTGC
GTGTGCGGCTGGACGTCAAGGATTTCGCGACCCCTGTGCAGTTCGTCAGTGCGTCGGCCACGGGCGACAAGGCCAGTATT
CTGATCGAGCCGTCGGGTGCCTTCGATTACTCTGCGTTCCAGACCGAAAACAAGCTGACCATCAGCGTACGTCCGTTGAC
CAACGAAGATCTGGACCGCCGCGCCGCCGACAAGCCGGTGTACACCGGTGAAAAGCTCTCGCTTAACTTCCAGGACATCG
ACGTGCGTTCGGTGCTGCAACTGATCGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGACACGGTTCAGGGCGGCATC
ACCCTGCGCCTGCAGAACGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAAGGCCTGGACAAGCGCAAGGT
CGGCAGTGTTCTGCTGGTTGCGCCAGCGGATGAAATTGCCGCCCGCGAACGCCAGGAGCTGGAATCGCTCAAGCAGATCG
CCGAACTGGCACCGCTGCGCCGCGAACTGCTGCAGGTCAATTACGCCAAGGCCGCCGACATCGCCAAGCTGTTCCAGTCG
GTGACCAGTGCCGAGTCCAAGGCTGAAGAGCGTGGCTCGATCACCGTGGACGACCGCACCAACAACATCATTGCCTACCA
GACCCAGGACCGTCTCGACGAACTGCGTCGCATCGTTTCACAACTGGACATTCCGGTGCGCCAGGTGATGATCGAAGCAC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAGTTGGGCGTGCGCTGGGGCGGTTCGACCAACACCAGCGGCAGCGGG
AAATGGACGACTTACGGGCTGGATGACAATGGCGATGAGGCCGGTAACACGAGCGGCAATCTGACTTCCAACGTTCCGTT
CGTGGACCTGGGGGCCGCCGGGGCGACGTCCGGCATCGGCCTGGGCTTTGTGACCAATAACACCTTGCTGGACCTTGAGC
TGAGCGCGATGGAGAAGACCGGCAACGGCGAAATCGTCTCCCAGCCCAAAGTGGTCACGTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGCACGGAGATTCCGTATCAGGAATCCAGCTCCAGCGGCGCCACCACCGTAAGCTTCAAGGAGGCTTCGCT
GTCGCTTGAAGTCACGCCGCAGATCACGCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACT
ACCTGAATGCGGTGCTTGGCGTACCGCCGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGATTTCCGATGGCGAAACC
ATCGTGATCGGTGGTGTGTTCTCCAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(536-692)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3M5TXK6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.752

100

0.75
  comQ Acinetobacter baylyi ADP1

42.06

99.715

0.419
  pilQ Acinetobacter baumannii strain A118

38.912

100

0.408
  pilQ Acinetobacter baumannii D1279779

38.912

100

0.408
  pilQ Legionella pneumophila strain Lp02

38.841

98.431

0.382
  pilQ Legionella pneumophila strain ERS1305867

38.653

97.432

0.377