Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   L1O00_RS01650 Genome accession   NZ_CP091140
Coordinates   349435..350874 (+) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas veronii strain b1-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 344435..355874
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1O00_RS01620 (L1O00_01620) mlaF 345012..345812 (-) 801 WP_005336343.1 phospholipid ABC transporter ATP-binding protein MlaF -
  L1O00_RS01625 (L1O00_01625) - 346020..347015 (+) 996 WP_058057518.1 KpsF/GutQ family sugar-phosphate isomerase -
  L1O00_RS01630 (L1O00_01630) kdsC 347015..347569 (+) 555 WP_101530526.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  L1O00_RS01635 (L1O00_01635) lptC 347566..348126 (+) 561 WP_005336338.1 LPS export ABC transporter periplasmic protein LptC -
  L1O00_RS01640 (L1O00_01640) lptA 348113..348640 (+) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  L1O00_RS01645 (L1O00_01645) lptB 348644..349369 (+) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  L1O00_RS01650 (L1O00_01650) rpoN/rpoN1 349435..350874 (+) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  L1O00_RS01655 (L1O00_01655) hpf 350895..351182 (+) 288 WP_005336323.1 ribosome hibernation promoting factor -
  L1O00_RS01660 (L1O00_01660) ptsN 351185..351631 (+) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  L1O00_RS01665 (L1O00_01665) rapZ 351677..352543 (+) 867 WP_005336320.1 RNase adapter RapZ -
  L1O00_RS01670 (L1O00_01670) npr 352564..352836 (+) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  L1O00_RS01675 (L1O00_01675) - 352854..354758 (-) 1905 WP_278171285.1 PTS sugar transporter subunit IIA -
  L1O00_RS01680 (L1O00_01680) - 354866..355606 (-) 741 WP_278171287.1 KDGP aldolase family protein -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=647964 L1O00_RS01650 WP_005336331.1 349435..350874(+) (rpoN/rpoN1) [Aeromonas veronii strain b1-3]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=647964 L1O00_RS01650 WP_005336331.1 349435..350874(+) (rpoN/rpoN1) [Aeromonas veronii strain b1-3]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCGCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCCCAGGAGCAGATGCCG
GACGAGTTGCCGGTGGATACCACTTGGGACGAGGTTTACTCTGCCGGCACCGCCCAGAGCGCGGGCCCCATCCACGATGG
CGAAGATAGCGTCTATCAGGGTGAGACCACCGAAAACCTGCAGGATTATCTGCTGTGGCAGATGCGGCTCACACCGTTCA
GCGATGTGGATGCCGCCATCGCGCTCGCCATCATCGATGCCATCGACGAATCCGGTTATCTGACCGTTGCGCTGGAGGAT
ATCCAGGCTGCCGTTACCAGCGATGACGTAGAGGTGGAACTCGATGAGGTCGAGGCGGTACTCAAGCGGGTTCAGCACTT
CGATCCGGTCGGCATTGCTGCCCGTTCTGTGCAGGAGTGTTTGCTGATCCAGCTGGATCAGTATGACCCTCAGACCCCCT
GGCTCAATGAGGCCAAAGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACCCTCGCCCGCAAG
ACAAAACTCAAGGAGAGCGACCTCAAGGAGGTGCTGGATCTGATCCAGCTACTGGAGCCGCGTCCCGGCAACGGCATCAT
CCAGAGCGACTCCCAGTACGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCTGCCCCCCGCATCCGGGTCAACGAGACCTATGCTGCCATGGCCCGCAACGCCCGCAGCAGCACCGACACCCAGTTT
ATCCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAATGACATCGCCG
AAGCGGTGGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCCCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6N7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491