Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   L1999_RS00540 Genome accession   NZ_CP091110
Coordinates   105503..106885 (+) Length   460 a.a.
NCBI ID   WP_235741813.1    Uniprot ID   -
Organism   Neobacillus drentensis strain JC05     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 100503..111885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1999_RS00520 (L1999_00520) - 100779..101240 (+) 462 WP_235741809.1 CtsR family transcriptional regulator -
  L1999_RS00525 (L1999_00525) - 101268..101816 (+) 549 WP_235741810.1 UvrB/UvrC motif-containing protein -
  L1999_RS00530 (L1999_00530) - 101818..102888 (+) 1071 WP_235741811.1 protein arginine kinase -
  L1999_RS00535 (L1999_00535) clpC 102906..105347 (+) 2442 WP_235741812.1 ATP-dependent protease ATP-binding subunit ClpC -
  L1999_RS00540 (L1999_00540) radA 105503..106885 (+) 1383 WP_235741813.1 DNA repair protein RadA Machinery gene
  L1999_RS00545 (L1999_00545) disA 106889..107965 (+) 1077 WP_235741814.1 DNA integrity scanning diadenylate cyclase DisA -
  L1999_RS00550 (L1999_00550) - 108170..109261 (+) 1092 WP_235741815.1 PIN/TRAM domain-containing protein -
  L1999_RS00555 (L1999_00555) ispD 109320..110024 (+) 705 WP_235741816.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  L1999_RS00560 (L1999_00560) ispF 110161..110643 (+) 483 WP_235741817.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 50125.61 Da        Isoelectric Point: 6.8343

>NTDB_id=647762 L1999_RS00540 WP_235741813.1 105503..106885(+) (radA) [Neobacillus drentensis strain JC05]
MVKRKTKFICQECGYESPKWMGKCPGCGQWNKMVEEVEVTGAQRRGAFAHSQTGSNLLTKPMKINAIEMENEPRILTDLQ
ELNRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSSQLANKGNSVLYISGEESLRQTKLRAERLGISSENLLVYSETNLEE
INRTIDSENPSFVIIDSIQTIFHPEVTSAPGSVSQVRECTSELMRIGKTKGVAIFIVGHVTKEGSIAGPRLLEHMVDTVL
YFEGERHHTYRILRAVKNRFGSTNEMGIFEMKEFGLEEVENPSEIFLEERSRGAAGSTVVASMEGTRPVLVEIQALISPT
SFGNPRRMATGIDHNRVPLLMAVLEKRVGMLLANQDAYLKVAGGVKLDEPAIDLAIAVSIASSFRDRPTRPTDCIIGEVG
LTGEVRRVSRIEQRVQEAAKLGFERIILPANNLSGWTGPEGIKLIGVSSVSEALQAALGG

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=647762 L1999_RS00540 WP_235741813.1 105503..106885(+) (radA) [Neobacillus drentensis strain JC05]
ATGGTAAAAAGGAAAACAAAGTTCATCTGCCAAGAATGCGGTTATGAATCCCCGAAATGGATGGGGAAATGCCCAGGCTG
TGGACAATGGAATAAGATGGTTGAGGAAGTAGAAGTAACAGGAGCTCAAAGAAGAGGAGCATTTGCTCATTCACAAACCG
GTTCTAATCTATTAACCAAACCAATGAAGATCAATGCAATTGAAATGGAAAACGAACCGAGAATTTTAACTGACTTACAA
GAGCTAAACCGCGTATTGGGCGGTGGTGTCGTTAAGGGCTCGTTAGTTCTAATTGGCGGTGACCCGGGAATAGGGAAATC
TACACTTCTCTTGCAAGTATCCTCACAGCTCGCGAATAAAGGCAATTCTGTTCTTTACATATCTGGTGAAGAATCATTAA
GACAGACAAAGCTTCGTGCGGAACGGCTGGGGATTTCCTCAGAAAACCTATTAGTTTATTCAGAAACAAACCTTGAAGAA
ATAAATCGGACGATTGATAGTGAGAATCCCAGCTTTGTTATTATTGACTCTATCCAAACGATCTTCCATCCAGAGGTAAC
GTCAGCACCTGGAAGTGTTTCTCAGGTCCGGGAATGCACATCGGAATTGATGAGGATCGGCAAAACCAAAGGGGTCGCCA
TCTTTATTGTTGGGCATGTGACCAAGGAAGGTTCTATTGCCGGGCCAAGGCTTTTAGAACATATGGTCGATACCGTGCTT
TATTTTGAAGGGGAAAGACATCATACTTACCGAATCCTTAGAGCCGTCAAAAATCGTTTTGGCTCTACAAATGAAATGGG
CATTTTTGAAATGAAGGAATTTGGACTCGAAGAAGTAGAAAACCCATCAGAAATTTTCCTGGAAGAGCGATCTCGGGGGG
CAGCTGGATCGACGGTTGTCGCTTCAATGGAGGGAACGCGTCCGGTTCTCGTTGAAATTCAGGCACTTATCTCCCCCACG
AGTTTTGGAAATCCACGAAGAATGGCAACAGGAATTGATCATAATCGGGTACCACTTTTAATGGCAGTCTTGGAAAAACG
GGTTGGTATGCTTCTAGCCAATCAGGATGCCTATTTAAAGGTTGCTGGAGGAGTTAAGCTGGATGAACCAGCGATTGATT
TAGCGATTGCCGTAAGTATTGCTTCGAGCTTTAGGGATCGCCCGACAAGACCTACCGACTGTATTATCGGTGAAGTTGGT
TTGACTGGAGAGGTAAGAAGGGTGTCAAGAATTGAACAGCGCGTTCAGGAGGCAGCCAAATTAGGCTTTGAGCGCATTAT
TTTACCAGCTAATAATTTAAGTGGCTGGACGGGCCCAGAGGGGATTAAGCTTATAGGTGTTTCCTCCGTTAGTGAGGCAC
TGCAAGCAGCGTTAGGAGGGTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(368-432)

AAA domain (AAA_25)

(76-224)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

76.84

100

0.772
  radA Streptococcus mitis NCTC 12261

62.745

99.783

0.626
  radA Streptococcus pneumoniae Rx1

62.745

99.783

0.626
  radA Streptococcus pneumoniae D39

62.745

99.783

0.626
  radA Streptococcus pneumoniae R6

62.745

99.783

0.626
  radA Streptococcus pneumoniae TIGR4

62.745

99.783

0.626
  radA Streptococcus mitis SK321

62.745

99.783

0.626