Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   L1S45_RS02100 Genome accession   NZ_CP091091
Coordinates   454826..456265 (+) Length   479 a.a.
NCBI ID   WP_094697654.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain b2-100     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 449826..461265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1S45_RS02070 (L1S45_02070) mlaF 450403..451203 (-) 801 WP_005305979.1 phospholipid ABC transporter ATP-binding protein MlaF -
  L1S45_RS02075 (L1S45_02075) - 451411..452406 (+) 996 WP_005305976.1 KpsF/GutQ family sugar-phosphate isomerase -
  L1S45_RS02080 (L1S45_02080) kdsC 452406..452960 (+) 555 WP_017766397.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  L1S45_RS02085 (L1S45_02085) lptC 452957..453517 (+) 561 WP_010635927.1 LPS export ABC transporter periplasmic protein LptC -
  L1S45_RS02090 (L1S45_02090) lptA 453504..454031 (+) 528 WP_010635928.1 lipopolysaccharide transport periplasmic protein LptA -
  L1S45_RS02095 (L1S45_02095) lptB 454035..454760 (+) 726 WP_005305964.1 LPS export ABC transporter ATP-binding protein -
  L1S45_RS02100 (L1S45_02100) rpoN/rpoN1 454826..456265 (+) 1440 WP_094697654.1 RNA polymerase factor sigma-54 Machinery gene
  L1S45_RS02105 (L1S45_02105) hpf 456287..456574 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  L1S45_RS02110 (L1S45_02110) ptsN 456577..457023 (+) 447 WP_005305951.1 PTS IIA-like nitrogen regulatory protein PtsN -
  L1S45_RS02115 (L1S45_02115) rapZ 457066..457932 (+) 867 WP_005305946.1 RNase adapter RapZ -
  L1S45_RS02120 (L1S45_02120) npr 457953..458225 (+) 273 WP_005305943.1 PTS phosphocarrier protein NPr -
  L1S45_RS02125 (L1S45_02125) - 458241..460145 (-) 1905 WP_252603360.1 PRD domain-containing protein -
  L1S45_RS02130 (L1S45_02130) - 460250..460990 (-) 741 WP_206220871.1 KDGP aldolase family protein -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53834.79 Da        Isoelectric Point: 4.3584

>NTDB_id=647591 L1S45_RS02100 WP_094697654.1 454826..456265(+) (rpoN/rpoN1) [Aeromonas dhakensis strain b2-100]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPVDQVDVSQLDSSDAMAQDQMP
DELPVDTTWEEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVILEHMDLLGNRDYRTLARK
TKLKENDLKEVLDLIQQLEPRPGNGVIQSESQYVIPDVSVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=647591 L1S45_RS02100 WP_094697654.1 454826..456265(+) (rpoN/rpoN1) [Aeromonas dhakensis strain b2-100]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAAAACGACG
TTCAGGAGACCAGCCCGGTCGACCAGGTGGACGTCAGCCAGCTCGACTCCAGCGATGCCATGGCCCAGGATCAGATGCCG
GACGAACTGCCGGTGGATACCACCTGGGAAGAGGTTTATTCGGCCGGCACGGCCCAGAGCGCAGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGTGAAACCACCGAGAACCTGCAGGACTACCTGTTGTGGCAGATGCGCTTGACCCCGTTCA
GCGACGTGGATGCCGCCATCGCCCTCGCCATCATAGATGCCATCGACGAATCCGGTTACCTGACGGTGTCGCTTGAGGAT
ATTCAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAGCTCGATGAAGTGGAGGCCGTGCTCAAGCGGGTGCAACATTT
CGATCCGGTCGGTATCGCCGCCCGCTCGGTGCAGGAGTGCCTGCTGATCCAGCTGGGCCAGTACTCACCTGATCTGCCCT
GGCTCAACGAAGCCAGGGAGGTGATCCTTGAGCACATGGATCTGCTCGGCAACCGCGATTACCGCACCTTGGCCCGCAAG
ACCAAGCTGAAGGAGAACGACCTCAAGGAGGTGCTGGATCTCATCCAGCAGCTGGAGCCGCGGCCCGGCAACGGCGTCAT
CCAGAGCGAATCCCAGTACGTGATCCCGGATGTTTCCGTGGTCAAGAAGGGCAACAAGTGGGTGGTCGAGCTCAACCCTG
ACTCGGCCCCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCAGACAGGCCCGTACCAGTCAGGATACCCAGTTC
ATCCGCTCCCACCTGCAGGAAGCAAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTCTTCGAATACGGTGAAGAGGCCATGAAACCCATGGTGCTCAACGACATCGCCG
AAGCGGTGGAGATGCACGAGTCGACCATCTCGCGGGTGACCACCCAGAAGTACATGCACACGCCGCGCGGCATCTTTGAA
CTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.866

100

0.486