Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   L0U95_RS14465 Genome accession   NZ_CP091012
Coordinates   3106874..3108379 (+) Length   501 a.a.
NCBI ID   WP_027810400.1    Uniprot ID   A0A3N8YL34
Organism   Burkholderia cenocepacia strain CMCC(B)23006     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3101874..3113379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0U95_RS14440 (L0U95_14440) kdsD 3103105..3104088 (+) 984 WP_011546235.1 arabinose 5-phosphate isomerase KdsD -
  L0U95_RS14445 (L0U95_14445) - 3104088..3104624 (+) 537 WP_054928050.1 KdsC family phosphatase -
  L0U95_RS14450 (L0U95_14450) lptC 3104628..3105242 (+) 615 WP_054928051.1 LPS export ABC transporter periplasmic protein LptC -
  L0U95_RS14455 (L0U95_14455) lptA 3105263..3105928 (+) 666 WP_012329239.1 lipopolysaccharide transport periplasmic protein LptA -
  L0U95_RS14460 (L0U95_14460) lptB 3105925..3106701 (+) 777 WP_006477788.1 LPS export ABC transporter ATP-binding protein -
  L0U95_RS14465 (L0U95_14465) rpoN/rpoN1 3106874..3108379 (+) 1506 WP_027810400.1 RNA polymerase factor sigma-54 Machinery gene
  L0U95_RS14470 (L0U95_14470) hpf 3108538..3108897 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  L0U95_RS14475 (L0U95_14475) - 3109092..3109604 (+) 513 WP_027806888.1 PTS sugar transporter subunit IIA -
  L0U95_RS14480 (L0U95_14480) hprK 3109734..3110702 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  L0U95_RS14485 (L0U95_14485) rapZ 3110777..3111685 (+) 909 WP_006477783.1 RNase adapter RapZ -
  L0U95_RS14490 (L0U95_14490) - 3111713..3112348 (+) 636 WP_011546241.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54739.80 Da        Isoelectric Point: 4.8740

>NTDB_id=647131 L0U95_RS14465 WP_027810400.1 3106874..3108379(+) (rpoN/rpoN1) [Burkholderia cenocepacia strain CMCC(B)23006]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPTA
TNGSSTSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDGDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=647131 L0U95_RS14465 WP_027810400.1 3106874..3108379(+) (rpoN/rpoN1) [Burkholderia cenocepacia strain CMCC(B)23006]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTTGCGCTGACGCCTCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCACTACTCGAGAACGACGACGAATGGA
TCGCCAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCGCCCGCACAGAACACCGAGCCCACCGCG
ACCAACGGCAGCAGCACGTCCAGCGATCGCGCCGAGCGCGACGAGCCGGCCGGCGCCGACGAATACGACGGCTACGGGTC
CGGTGACGGCAACGACGGCCCGCAGTGGAATCTCGACGAATACGGCCGCTCGAGCGGCGCGTCCGACGACGACGACCTGC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGATCATCTCTCGGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAACTGGAAATCGACGGCGACGAACTGAATGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTCGGCGCGCGCTCGGCCTCCGAATGCCTGAAGCTGCAGCTGCTGCGGCTCGATCCGTCCCCCACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCACGCGACTTCACACGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCCTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCTGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGTTGAAGCAGCA
GCTCCAGGAAGCACGCTGGTTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGG
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3N8YL34

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689