Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L1O99_RS14720 Genome accession   NZ_CP090971
Coordinates   3409068..3410207 (+) Length   379 a.a.
NCBI ID   WP_012917187.1    Uniprot ID   D2UFN3
Organism   Xanthomonas albilineans strain Xa21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3404068..3415207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1O99_RS14695 - 3404314..3405498 (-) 1185 WP_012917182.1 cystathionine gamma-synthase -
  L1O99_RS14700 - 3405582..3406952 (-) 1371 WP_012917183.1 pyridoxal-phosphate dependent enzyme -
  L1O99_RS14705 - 3407132..3407350 (-) 219 WP_045757024.1 YdcH family protein -
  L1O99_RS14710 - 3407670..3408566 (-) 897 WP_012917185.1 OmpA family protein -
  L1O99_RS14715 - 3408563..3408961 (-) 399 WP_045757025.1 DUF4398 domain-containing protein -
  L1O99_RS14720 pilU 3409068..3410207 (+) 1140 WP_012917187.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L1O99_RS14725 maiA 3410261..3410920 (-) 660 WP_012917188.1 maleylacetoacetate isomerase -
  L1O99_RS14730 - 3411018..3412007 (-) 990 WP_012917189.1 fumarylacetoacetate hydrolase family protein -
  L1O99_RS14735 - 3412354..3413724 (+) 1371 WP_012917190.1 DEAD/DEAH box helicase -
  L1O99_RS14740 queD 3413874..3414236 (+) 363 WP_012917191.1 6-carboxytetrahydropterin synthase QueD -
  L1O99_RS14745 - 3414437..3414814 (+) 378 WP_045757401.1 phasin family protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42162.37 Da        Isoelectric Point: 5.8058

>NTDB_id=646772 L1O99_RS14720 WP_012917187.1 3409068..3410207(+) (pilU) [Xanthomonas albilineans strain Xa21]
MDIGYFLKLMTEKNASDMFLTTGAPIYIKIEGKLYPLGATGLPPGMVKKIAYSLMDEGQVPQFERDLELNMAVAVQDAGR
FRVNAFKQRGEVGMVIRAIRSKIPSIEELHLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDFRNSTTTGHILTIED
PIEYLHKHKLSIVNQREVGLDTHAFQNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLSLNLRAVISQRLVKDKDGRRRPASEVLLNTPMIRDLLRRGQVHEIKQAMEESLEEGMETFDQCL
FRMVKEDKIDQEAALRAADSRDGLALKFRLSEGASGEHNPYEDYENDTGASSQITHGFV

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=646772 L1O99_RS14720 WP_012917187.1 3409068..3410207(+) (pilU) [Xanthomonas albilineans strain Xa21]
ATGGATATCGGCTACTTTCTGAAGCTGATGACCGAAAAGAACGCCTCGGACATGTTCCTGACAACGGGAGCACCGATCTA
TATCAAGATCGAGGGCAAACTGTATCCACTCGGCGCTACTGGCCTGCCGCCGGGGATGGTCAAAAAAATCGCCTACTCGC
TGATGGACGAGGGACAGGTACCCCAGTTCGAACGCGATCTGGAACTCAATATGGCTGTCGCCGTGCAGGACGCAGGACGC
TTCCGCGTCAACGCGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGGGCCATTCGCAGCAAGATTCCCAGCATTGA
GGAGCTGCATCTGCCACAGGTCCTCAAGGACGTAATCATGACCCCGCGCGGATTGGTCCTGGTGGTCGGCTCGACCGGCT
CGGGCAAGTCCACCTCGCTGGCATCGATGATCGATTTCCGCAACAGCACCACCACCGGCCATATCCTCACCATCGAGGAT
CCGATCGAGTACCTACACAAACATAAGCTGTCCATCGTCAACCAACGCGAAGTCGGCCTGGACACCCATGCTTTCCAAAA
CGCGTTGAAGAATGCGATGCGCGAAGCACCGGATGTGATCCTGATCGGCGAAATTCTGGACGCAGAGACGATGGAAGCGG
CCATCGCCTTCGCCGAGACAGGCCATCTGTGTCTGGCGACCTTGCACTCCAACAACGCCGACCAGACGATCGAACGCATC
CTCAATTTCTTTCCCGAGAGCGCACACAAGAACGTTCTGATGAACCTGTCGCTGAATCTGCGCGCAGTGATCTCGCAACG
GCTGGTCAAGGACAAAGATGGGCGCCGCCGCCCCGCCTCGGAAGTGCTGTTGAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTCCACGAGATCAAGCAGGCGATGGAGGAATCGCTGGAAGAGGGCATGGAAACCTTCGACCAGTGTTTG
TTCCGGATGGTCAAGGAGGACAAGATCGATCAGGAAGCGGCACTGCGCGCGGCCGACTCGCGCGACGGCCTGGCGCTGAA
GTTCCGCTTGTCCGAGGGCGCCAGCGGCGAGCACAACCCCTACGAAGACTACGAAAACGACACCGGCGCCTCGTCGCAGA
TCACACACGGCTTCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D2UFN3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.532

94.723

0.488
  pilU Vibrio cholerae strain A1552

51.412

93.404

0.48
  pilU Acinetobacter baylyi ADP1

50.14

94.195

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.81

94.459

0.433
  pilT Pseudomonas aeruginosa PAK

43.713

88.127

0.385
  pilT Acinetobacter baumannii strain A118

42.308

89.182

0.377
  pilT Acinetobacter nosocomialis M2

42.308

89.182

0.377
  pilT Acinetobacter baumannii D1279779

42.308

89.182

0.377
  pilT Legionella pneumophila strain Lp02

41.42

89.182

0.369
  pilT Legionella pneumophila strain ERS1305867

41.42

89.182

0.369
  pilT Pseudomonas stutzeri DSM 10701

41.916

88.127

0.369
  pilT Neisseria gonorrhoeae MS11

40.058

91.557

0.367
  pilT Acinetobacter baylyi ADP1

40.882

89.71

0.367
  pilT Neisseria meningitidis 8013

39.769

91.557

0.364