Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   L0U97_RS02770 Genome accession   NZ_CP090890
Coordinates   575135..575935 (+) Length   266 a.a.
NCBI ID   WP_004908991.1    Uniprot ID   -
Organism   Acinetobacter junii strain WCO-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 570135..580935
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0U97_RS02745 - 571044..572360 (+) 1317 WP_234856876.1 glycosyltransferase family 4 protein -
  L0U97_RS02750 - 572388..572939 (+) 552 WP_004908998.1 phosphatase PAP2 family protein -
  L0U97_RS02755 iscX 573022..573222 (+) 201 WP_042891252.1 Fe-S cluster assembly protein IscX -
  L0U97_RS02760 ndk 573330..573761 (+) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  L0U97_RS02765 rlmN 573883..575118 (+) 1236 WP_042891255.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  L0U97_RS02770 pilF 575135..575935 (+) 801 WP_004908991.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  L0U97_RS02775 - 575926..576717 (+) 792 WP_004950312.1 helix-turn-helix domain-containing protein -
  L0U97_RS02780 ispG 576742..577857 (+) 1116 WP_004908985.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  L0U97_RS02785 hisS 577854..579146 (+) 1293 WP_039047056.1 histidine--tRNA ligase -
  L0U97_RS02790 - 579178..579876 (+) 699 WP_004950316.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30366.44 Da        Isoelectric Point: 9.5600

>NTDB_id=646193 L0U97_RS02770 WP_004908991.1 575135..575935(+) (pilF) [Acinetobacter junii strain WCO-9]
MNTTRLKTILATTVVFTVLGLTACQSTTLRKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKIDPRDATANMMMGVL
LQQEGSKPNMEKAEAYFKRAISSEPDNAQARNNYGTYLYQMQRYNEAIEQFTVAGATLGYEQRYQSLENLGRIYLKLGDV
ANAEKSFKQALQANRNSTISMLELSEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGIRIARANADQLGMQVLVNQ
MRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=646193 L0U97_RS02770 WP_004908991.1 575135..575935(+) (pilF) [Acinetobacter junii strain WCO-9]
TTGAATACAACTCGACTAAAAACGATTTTAGCTACTACTGTTGTGTTTACTGTATTAGGTTTGACTGCCTGCCAATCAAC
AACGTTAAGAAAAGATCCAGAAAAGGCAGTACAAGTCCGAACTCAACTCGCTGCTGAATATATTCGAACACGTGATTTGG
ATTCAGCGAAACGCTCCCTTGATCAAGCTTTAAAAATTGATCCACGTGATGCTACTGCCAATATGATGATGGGTGTTTTG
TTGCAACAAGAAGGCAGCAAACCAAACATGGAGAAAGCAGAAGCTTATTTTAAGCGAGCAATCTCTTCAGAACCCGATAA
TGCCCAAGCTCGTAATAATTACGGTACATATTTGTATCAAATGCAACGTTATAATGAGGCGATTGAGCAATTTACCGTTG
CAGGTGCGACGTTAGGTTACGAACAGCGTTACCAATCGTTGGAAAACTTGGGTCGAATCTATTTAAAACTGGGTGATGTT
GCGAATGCTGAAAAATCATTCAAGCAAGCCTTACAAGCAAATCGAAATTCGACAATTTCAATGTTAGAGTTGTCCGAAAT
TTTCTATTTGCAACAAAATAATAGAGATGCATCGCAACTTTATGAACAATACGTTCGTAATGTCGGGCAGAAAAATCAAG
GTGCTCGTGCACTTTGGATCGGTATACGCATTGCTCGAGCGAATGCGGATCAATTAGGAATGCAAGTATTAGTGAATCAA
ATGCGTGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-69)

Tetratricopeptide repeat (TPR_8)

(145-176)

Tetratricopeptide repeat (TPR_16)

(90-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.026

100

0.793
  pilF Acinetobacter baylyi ADP1

68.045

100

0.68