Detailed information NTG0064550

Bioinformatically predicted

Overview

• Name: amiF
• Type: Regulator
• Locus tag: SPB_RS06750
• Genome accession: NZ_AEUT02000001
• Coordinates: 1356263..1357198 (-)
• Length: 311 a.a.
• NCBI ID: WP_003105521.1
• Uniprot ID: A0A2I8AI54
• Organism: Streptococcus parauberis NCFD 2020
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 1351263..1362198

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPB_RS06725 (SPB_1389)	tsaD	1351894..1352907 (-)	1014	WP_003105067.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD	-
SPB_RS06730 (SPB_1390)	rimI	1352897..1353313 (-)	417	WP_003105855.1	ribosomal protein S18-alanine N-acetyltransferase	-
SPB_RS06735 (SPB_1391)	tsaB	1353318..1354010 (-)	693	WP_302621693.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
SPB_RS06740 (SPB_1392)	-	1354283..1354513 (+)	231	WP_003102975.1	DNA-dependent RNA polymerase subunit epsilon	-
SPB_RS06745 (SPB_1393)	-	1354515..1356197 (+)	1683	WP_003104437.1	ribonuclease J1	-
SPB_RS06750 (SPB_1394)	amiF	1356263..1357198 (-)	936	WP_003105521.1	ABC transporter ATP-binding protein	Regulator
SPB_RS06755 (SPB_1395)	oppD	1357198..1358241 (-)	1044	WP_003103717.1	ABC transporter ATP-binding protein	Regulator
SPB_RS06760 (SPB_1396)	-	1358256..1359287 (-)	1032	WP_003102826.1	ABC transporter permease	-
SPB_RS06765 (SPB_1397)	-	1359297..1360211 (-)	915	WP_003103193.1	ABC transporter permease	-
SPB_RS06770 (SPB_1398)	-	1360287..1361945 (-)	1659	WP_003106046.1	peptide ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 311 a.a.
• Molecular weight: 35205.16 Da
• Isoelectric Point: 6.5131

>NTDB_id=64550 SPB_RS06750 WP_003105521.1 1356263..1357198(-) (amiF) [Streptococcus parauberis NCFD 2020]
MTEERKKLVELKNVSLTFNKGKSNEVKAINNVSFDIYEGEVFGLVGESGSGKTTVGRAILKLYEITDGEIIFNGVNITKL
KGKELHNFRKDAQMIFQDPQASLNGRMKIRDIVAEGLDIHGLTKTKEEREARVQELLDRVGLNKDHVTRYPHEFSGGQRQ
RIGIARALAVKPKFIIADEPISALDVSIQAQVVNLMQKLQTERGLTYLFIAHDLSMVKYISDRIGVMHWGKMLEIGTSED
VYNNPIHPYTRSLLSAIPEPDPDYERQRVAEIYDPSIELDGKEREMREITPGHFVFSTEEEAVAYKANSNK

Nucleotide

• Length: 936 bp

>NTDB_id=64550 SPB_RS06750 WP_003105521.1 1356263..1357198(-) (amiF) [Streptococcus parauberis NCFD 2020]
ATGACTGAAGAACGTAAAAAATTAGTTGAACTAAAAAACGTTTCTTTAACCTTTAATAAAGGGAAAAGCAACGAAGTCAA
GGCCATTAATAATGTCAGTTTTGATATCTATGAAGGTGAAGTCTTTGGATTAGTTGGGGAATCAGGTTCTGGTAAAACAA
CAGTTGGCCGTGCTATCTTAAAACTTTATGAAATTACCGATGGCGAAATTATTTTCAATGGTGTAAATATCACTAAACTA
AAAGGAAAAGAACTTCATAATTTCCGTAAAGATGCACAAATGATTTTCCAAGATCCACAAGCAAGTCTTAATGGACGGAT
GAAAATTAGAGACATCGTTGCTGAAGGACTAGATATTCATGGTTTAACTAAAACAAAGGAAGAACGTGAAGCTCGTGTTC
AAGAACTACTTGACCGTGTTGGTTTGAATAAAGACCATGTGACTCGTTATCCACATGAATTTTCAGGAGGACAACGTCAA
CGTATTGGTATTGCCCGTGCCTTGGCTGTAAAACCCAAATTTATCATTGCTGATGAACCAATTTCTGCTTTGGACGTATC
TATCCAAGCACAGGTTGTTAACTTGATGCAAAAATTACAAACTGAACGTGGTTTAACCTACTTGTTTATCGCCCATGATT
TATCAATGGTTAAATACATTTCAGATCGTATTGGTGTTATGCACTGGGGTAAAATGTTAGAAATTGGGACATCTGAAGAT
GTTTATAATAACCCAATTCACCCATATACAAGAAGTCTCTTATCAGCTATTCCTGAACCAGACCCAGATTATGAACGCCA
ACGTGTTGCGGAAATTTATGATCCAAGTATTGAATTGGATGGAAAAGAACGTGAAATGCGTGAAATCACACCAGGTCACT
TCGTATTTTCAACAGAAGAAGAAGCAGTAGCTTACAAAGCAAATTCAAATAAATAA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (30-181)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-265)

3D structure

• AlphaFold DB: A0A2I8AI54

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiF	Streptococcus thermophilus LMD-9	54.221	99.035	0.537
amiF	Streptococcus thermophilus LMG 18311	53.896	99.035	0.534
amiF	Streptococcus salivarius strain HSISS4	53.247	99.035	0.527